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cholestyramine resin  (MedChemExpress)
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MedChemExpress cholestyramine resin
MWD promotes colitis development in their progeny by producing DCA. a As shown in Fig. a, WT mice ( n = 5) were colonized with gut microbiota from the W-N and N-N groups. Then, the concentration of DCA in the mice’s feces was measured using an ELISA assay kit. b – g <t>Cholestyramine</t> resin (resin) was given to mice in the W-N and N-N groups ( n = 6 per group) for 5 days to eliminate intestinal bile acids. Following that, mice were given TNBS to induce colitis. c Body weight changes in mice were evaluated daily after TNBS treatment. d Representative images of TNBS-treated colon in N-N+resin and W-N+resin groups. e The mice were sacrificed on day 4, and the colon length was recorded. f , g Histopathological analysis of colon sections. f Histological scores of colitis were assessed. g Representative images of the H&E-stained colon sections of relevant groups (scale bars 100 μm). a , c , e , and f Data represent means ± SEM; NS, not significant; ** P < 0.01; by unpaired Student’s t test. The data shown are representative of three independent experiments
Cholestyramine Resin, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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MWD promotes colitis development in their progeny by producing DCA. a As shown in Fig. a, WT mice ( n = 5) were colonized with gut microbiota from the W-N and N-N groups. Then, the concentration of DCA in the mice’s feces was measured using an ELISA assay kit. b – g Cholestyramine resin (resin) was given to mice in the W-N and N-N groups ( n = 6 per group) for 5 days to eliminate intestinal bile acids. Following that, mice were given TNBS to induce colitis. c Body weight changes in mice were evaluated daily after TNBS treatment. d Representative images of TNBS-treated colon in N-N+resin and W-N+resin groups. e The mice were sacrificed on day 4, and the colon length was recorded. f , g Histopathological analysis of colon sections. f Histological scores of colitis were assessed. g Representative images of the H&E-stained colon sections of relevant groups (scale bars 100 μm). a , c , e , and f Data represent means ± SEM; NS, not significant; ** P < 0.01; by unpaired Student’s t test. The data shown are representative of three independent experiments

Journal: Microbiome

Article Title: Maternal Western diet mediates susceptibility of offspring to Crohn’s-like colitis by deoxycholate generation

doi: 10.1186/s40168-023-01546-6

Figure Lengend Snippet: MWD promotes colitis development in their progeny by producing DCA. a As shown in Fig. a, WT mice ( n = 5) were colonized with gut microbiota from the W-N and N-N groups. Then, the concentration of DCA in the mice’s feces was measured using an ELISA assay kit. b – g Cholestyramine resin (resin) was given to mice in the W-N and N-N groups ( n = 6 per group) for 5 days to eliminate intestinal bile acids. Following that, mice were given TNBS to induce colitis. c Body weight changes in mice were evaluated daily after TNBS treatment. d Representative images of TNBS-treated colon in N-N+resin and W-N+resin groups. e The mice were sacrificed on day 4, and the colon length was recorded. f , g Histopathological analysis of colon sections. f Histological scores of colitis were assessed. g Representative images of the H&E-stained colon sections of relevant groups (scale bars 100 μm). a , c , e , and f Data represent means ± SEM; NS, not significant; ** P < 0.01; by unpaired Student’s t test. The data shown are representative of three independent experiments

Article Snippet: Cholestyramine resin (11,041–12-6) was obtained from MedChem Express.

Techniques: Concentration Assay, Enzyme-linked Immunosorbent Assay, Staining