Journal: BMC Pharmacology & Toxicology

Article Title: Depressive effectiveness of vigabatrin (γ-vinyl-GABA), an antiepileptic drug, in intermediate-conductance calcium-activated potassium channels in human glioma cells

doi: 10.1186/s40360-021-00472-3

Figure Lengend Snippet: Effect of VGB on the activity of intermediate-conductance Ca 2+ -activated K + (IK Ca ) channels expressed in human 13–06-MG glioma cells. In this set of experiments, 13–06-MG cells were bathed in normal Tyrode’s solution containing 1.8 mM CaCl 2 and single-channel current recordings were made. The probability of IK Ca channels opening was measured at − 80 mV relative to the bath. a Original current traces for IK Ca channels obtained in the absence (left) and presence (right) of VGB (10 μM). Note that channel opening gives a downward deflection in current. b Original IK Ca channel traces taken in the absence (upper) and presence (lower) of TRAM-34 (3 μM). c Summary of the data showing the effects of control, extracellular Ca 2+ (0 mM), extracellular Ca 2+ (3.6 mM), VGB, TRAM-34 (3 μM), and VGB (10 μM) plus DCEBIO (10 μM) on IK Ca channel activity. The probability of IK Ca channel opening was measured at − 80 mV relative to the bath. Each bar indicates the mean ± SEM ( n =9–11). * Significantly different from control (i.e., in the presence of 1.8 mM Ca 2+ , but VGB was not present) ( P < 0.05) and ** significantly different from the VGB alone group ( P < 0.05)

Article Snippet: VGB ((±)-γ-vinyl-GABA, C 6 H 11 NO 2 ) was acquired from Sigma-Aldrich (Merck Ltd., Taipei, Taiwan), GAL-021 was from MedChemExpress (Everything Biotech Ltd., New Taipei City, Taiwan), while DCEBIO (5,6-dichloro-1-ethyl-1,3-dihydro-2 H -benzimidazol-2-one) and TRAM-34 (1-((2-chlorophenyl)-(diphenyl)methyl)-1 H -pyrazole) were from Tocris (Union Biomed, Taipei, Taiwan).

Techniques: Activity Assay