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Journal: EMBO Reports
Article Title: Tudor-based proteomic strategy pan-specifically enriches and identifies protein arginine methylation
doi: 10.1038/s44319-025-00599-y
Figure Lengend Snippet: ( A ) Schematic diagram depicting the Tudor affinity resin. SMN Tudor domain shown in surface representation (light gray) is immobilized onto the Halo-link agarose resin via an N-terminal Halo-tag. The box on the right illustrates the recognition of sDMA by the aromatic cage of SMN Tudor domain. ( B ) Workflow for the molecular affinity strategy for arginine methylation profiling. ( C ) The enrichment efficiencies of molecular affinity strategy in three biological replicate experiments. ( D ) The number of methylation peptides and different methylarginine sites (monomethyl or dimethyl) identified from three biological replicates. ( E ) The reproducibility of molecular affinity strategy by comparing identified methylarginine sites in three biological replicates.
Article Snippet:
Techniques: Methylation
Journal: EMBO Reports
Article Title: Tudor-based proteomic strategy pan-specifically enriches and identifies protein arginine methylation
doi: 10.1038/s44319-025-00599-y
Figure Lengend Snippet: The lines represent data fitting curves using 1:1 binding model at a fixed stoichiometry (N) of 1. The peptides used were SmD1 (AGRGRGR), SmD1-sDMA (AGR me2s GRGR), SmD1 aDMA (AGR me2a GRGR).
Article Snippet:
Techniques: Binding Assay