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Journal: International Journal of Molecular Sciences
Article Title: IL-4 and IL-13 Promote Proliferation of Mammary Epithelial Cells through STAT6 and IRS-1
doi: 10.3390/ijms222112008
Figure Lengend Snippet: IRS-1 inhibitor NT157 suppresses IL-4- and IL-13-stimulated cell proliferation. Mammary cells cultured on a basement membrane (BM)-like matrix, Matrigel, were untreated or treated with NT157 (5 μM), IL-4 or IL-13 for 2 d. ( A ) Total cell lysates were analyzed by immunoblotting with antibodies to IRS-1 and actin. Actin was used as a loading control. Data were quantified, normalized to loading control and expressed as fold change relative to control ( n = 4). * p < 0.05, ** p < 0.01. ( B ) Prior to harvesting, cells were pulsed with EdU (10 μM; green) for 8 h. Nuclei were stained with Hoechst 33342 (blue). Scale bar, 100 μm.
Article Snippet:
Techniques: Cell Culture, Membrane, Western Blot, Control, Staining
Journal: Cell Reports Methods
Article Title: RECOVER identifies synergistic drug combinations in vitro through sequential model optimization
doi: 10.1016/j.crmeth.2023.100599
Figure Lengend Snippet:
Article Snippet:
Techniques: Software, Recombinant