Journal: Journal of Cancer

Article Title: Serum Adiponectin Level May be an Independent Predictor of Clear Cell Renal Cell Carcinoma

doi: 10.7150/jca.14716

Figure Lengend Snippet: Serum adiponectin level, leptin level and VFA in different subtypes of renal cell carcinoma. The mean and standard deviation data were plotted.

Article Snippet: The detection of plasma leptin concentration was the same as that described above, using a human-specific leptin ELISA kit (EK1972, Multisciences, Shanghai, China).

Techniques: Standard Deviation

Journal: Journal of Cancer

Article Title: Serum Adiponectin Level May be an Independent Predictor of Clear Cell Renal Cell Carcinoma

doi: 10.7150/jca.14716

Figure Lengend Snippet: Differences of serum adiponectin, leptin level and VFA between ccRCC patients and non-ccRCC patients.

Article Snippet: The detection of plasma leptin concentration was the same as that described above, using a human-specific leptin ELISA kit (EK1972, Multisciences, Shanghai, China).

Techniques:

Journal: Journal of Cancer

Article Title: Serum Adiponectin Level May be an Independent Predictor of Clear Cell Renal Cell Carcinoma

doi: 10.7150/jca.14716

Figure Lengend Snippet: The association of VFA with serum adiponectin and leptin level. Pearson's tests show that the correlation between VFA and serum adiponectin level (p < 0.001) and the link between VFA and serum leptin concentration (p < 0.001) are statistically significant.

Article Snippet: The detection of plasma leptin concentration was the same as that described above, using a human-specific leptin ELISA kit (EK1972, Multisciences, Shanghai, China).

Techniques: Concentration Assay

Journal: Journal of Cancer

Article Title: Serum Adiponectin Level May be an Independent Predictor of Clear Cell Renal Cell Carcinoma

doi: 10.7150/jca.14716

Figure Lengend Snippet: Univariate analysis and multivariate analysis of predictors for different subtypes of RCC.

Article Snippet: The detection of plasma leptin concentration was the same as that described above, using a human-specific leptin ELISA kit (EK1972, Multisciences, Shanghai, China).

Techniques:

Journal: Journal of Cancer

Article Title: Serum Adiponectin Level May be an Independent Predictor of Clear Cell Renal Cell Carcinoma

doi: 10.7150/jca.14716

Figure Lengend Snippet: Association between clinical parameters and plasma adiponectin/leptin levels in the ccRCC subgroup.

Article Snippet: The detection of plasma leptin concentration was the same as that described above, using a human-specific leptin ELISA kit (EK1972, Multisciences, Shanghai, China).

Techniques: Clinical Proteomics

Journal: Journal of Cancer

Article Title: Serum Adiponectin Level May be an Independent Predictor of Clear Cell Renal Cell Carcinoma

doi: 10.7150/jca.14716

Figure Lengend Snippet: Mann-Whitney U test of serum adiponectin level, Student's t test of VFA and serum leptin level in patients with ccRCC and non-ccRCC. Patients with ccRCC have a significantly lower plasma adiponectin level (p = 0.004) and higher VFA (p = 0.044) than non-ccRCC patients, while no differences are seen in mean plasma leptin level (p = 0.940).

Article Snippet: The detection of plasma leptin concentration was the same as that described above, using a human-specific leptin ELISA kit (EK1972, Multisciences, Shanghai, China).

Techniques: MANN-WHITNEY, Clinical Proteomics

Journal: Journal of Cancer

Article Title: Serum Adiponectin Level May be an Independent Predictor of Clear Cell Renal Cell Carcinoma

doi: 10.7150/jca.14716

Figure Lengend Snippet: Patient demographic and clinical characteristics.

Article Snippet: The detection of plasma leptin concentration was the same as that described above, using a human-specific leptin ELISA kit (EK1972, Multisciences, Shanghai, China).

Techniques:

Journal: eBioMedicine

Article Title: Exercise-derived peptide protects against pathological cardiac remodeling

doi: 10.1016/j.ebiom.2022.104164

Figure Lengend Snippet: The primers used in this study.

Article Snippet: The concentrations of CCDC80tide in EVs were measured by ELISA (Human CCDC80 ELISA Kit, EK1962, Boster Bio, USA) combined with immunoblotting assay.

Techniques:

Journal: eBioMedicine

Article Title: Exercise-derived peptide protects against pathological cardiac remodeling

doi: 10.1016/j.ebiom.2022.104164

Figure Lengend Snippet: CCDC80 is a potential exerkine involved in cardiovascular pathophysiology . a. Circos plot visualizes the overlap between upregulated gene lists from three databases, and the blue curves link genes that belong to the same enriched GO term. b. Pie charts represent the network of enriched terms in upregulated genes. Each circle node represents an enriched term and is clustered by its identity, where the size of a slice represents the percentage of genes under the term that originated from the corresponding database. c. Heatmap of enriched terms across the three upregulated gene lists, colored by p -values. d. Heatmap of enrichment analysis in DisGeNET, colored by p-values. e. Circos plot displays the overlap between upregulated gene lists, and the purple curves link identical genes. The five genes within the black box represent the genes with significant upregulation throughout the three databases. f. RT-PCR analysis of the expression levels of CCDC80 in various tissues of mice. g, h. After overexpression of PGC-1α in C2C12 cells, the expressions of Pgc-1α (g) and Ccdc80 (h) was determined by qPCR. i, j and k. Eight-week-old male mice were forced to swim 5 days per week for 3 months. Control groups mice were raised normally without swimming training. After complete swimming training, the skeletal muscles of mice were collected for western blot analysis of CCDC80 and PGC-1α ( n= 7). Data are presented as the average of four biological replicates (n = 4) ± SD. Statistical significance was determined using Student's t-test, *** p < 0.001.

Article Snippet: The concentrations of CCDC80tide in EVs were measured by ELISA (Human CCDC80 ELISA Kit, EK1962, Boster Bio, USA) combined with immunoblotting assay.

Techniques: Reverse Transcription Polymerase Chain Reaction, Expressing, Over Expression, Control, Muscles, Western Blot

Journal: eBioMedicine

Article Title: Exercise-derived peptide protects against pathological cardiac remodeling

doi: 10.1016/j.ebiom.2022.104164

Figure Lengend Snippet: CCDC80 generates a peptide encapsulated in EVs in response to PGC-1α overexpression . a. The cell lysates and culture supernatants collected from vehicle- or Flag-PGC-1α-overexpressing C2C12 cells (treated or not with BFA, 1 μg/mL, 16 h) were prepared and analyzed for CCDC80 protein levels by WB. N= 3 b. The supernatants of CCDC80-EGFP-overexpressing C2C12 cells were harvested and filtered through a sterile 0.22-μm filter and then added to H9c2 cells. After 12-h incubation, the cell fluorescence was detected. The fluorescent spots indicated by white arrows were supposedly EVs. N= 3 c, d, e. The supernatants of CCDC80-overexpressing C2C12 cells were collected, and EVs were extracted for TEM (c), NTA (d), and EVs markers (e) determinations. N= 3. WCL: whole cell lysates. f. The cell lysates and EVs isolated from the supernatants of vehicle- or CCDC80-HA-overexpressing C2C12 cells were prepared and analyzed for CCDC80 protein levels by WB. g. The schematic diagram of CCDC80tide in CCDC80.

Article Snippet: The concentrations of CCDC80tide in EVs were measured by ELISA (Human CCDC80 ELISA Kit, EK1962, Boster Bio, USA) combined with immunoblotting assay.

Techniques: Over Expression, Sterility, Incubation, Fluorescence, Isolation

Journal:

Article Title: NAD-Dependent DNA-Binding Activity of the Bifunctional NadR Regulator of Salmonella typhimurium

doi:

Figure Lengend Snippet: Strains used

Article Snippet: EK193 , EK192(DE3) λ lysogen; lacUV5 controlled expression of T7 RNA polymerase , Novagen.

Techniques: Expressing, Transformation Assay

Journal: Nature Communications

Article Title: Single-cell transcriptomics analysis of bullous pemphigoid unveils immune-stromal crosstalk in type 2 inflammatory disease

doi: 10.1038/s41467-024-50283-3

Figure Lengend Snippet: a Volcano plot of gene features of the CCL19 + FB cluster in BP patients compared to HC. b Volcano plot of gene features of the APCDD1 + FB cluster in BP patients compared to HC. P -values in a and b were obtained using the two-sided Likelihood-ratio test and Bonferroni corrected (Seurat 4). c The feature plot and the bar chart showing PLA2G2A mRNA expression within total skin cells between BP ( n = 5) and HC ( n = 8). d Protein level of PLA2G2A in serum from BP ( n = 73) and HC ( n = 31) examined by ELISA. e Transwell assays were used to measure cell migration of THP1 ( n = 9) and Jurkat ( n = 9) cells treated by PLA2G2A recombinant protein. P -values in c – e were calculated using two-sided Mann–Whitney U-test. * P < 0.05, ** P < 0.01, **** P < 0.0001. In the box plot c – e : Minima: Lower limit of the whisker. Maxima: Upper limit of the whisker. Center: Median line inside the box. The upper and lower box bounds represent the 25% and 75% percentile of data. f Representative images of a BP patient and HC stained by multicolored IHC; green represents PLA2G2A + fibroblasts, red represents macrophages, and yellow represents CD3 T cells. scale bar = 50 μm. Data are from three independent experiments. g Circle plots of the inferred CXCL12 - CXCR4 pathway among major cell types in the BP and HC groups. h Hierarchical plot showing inferred intercellular communication network of CXCL12 - CXCR4 signaling in BP skin. i The PLA2G2A / CXCL12 gene pair co-expression (upper panel), and the gene expression correlation analysis in expressing both genes (lower panel) in fibroblasts. The correlation was measured using the Pearson correlation coefficient. P -values were calculated using two-sided Pearson correlation test. j Flow plots of CD3 + T cells from PBMCs showing the expression of CXCR4 treated by PLA2G2A recombinant protein (upper panel), and the frequency of CXCR4 (lower panel) in BP ( n = 21) and HC ( n = 13) groups. P -values were calculated using paired two-sided Student’s t -test. ** P < 0.01. Each sample is represented as one dot.

Article Snippet: The serum levels of IL-13 (EK0424), IL-4 (EK0404), IL-5 (EK0407), PLA2G2A (EK1944), and CCL17 (EK0684) in both patients and controls were quantified using ELISA Development Kits from BosterBio, USA, following the manufacturer’s instructions.

Techniques: Expressing, Enzyme-linked Immunosorbent Assay, Migration, Recombinant, MANN-WHITNEY, Whisker Assay, Staining, Gene Expression

Journal: Nature Communications

Article Title: Single-cell transcriptomics analysis of bullous pemphigoid unveils immune-stromal crosstalk in type 2 inflammatory disease

doi: 10.1038/s41467-024-50283-3

Figure Lengend Snippet: a Hierarchical plot showing inferred intercellular communication network of CCL17 - CCR4 signaling. b The feature plot and the bar chart showing CCL17 mRNA expression within total skin cells between BP ( n = 5) and HC ( n = 8). c Protein level of CCL17 in serum from BP ( n = 73) and HC ( n = 32) by ELISA. d The feature plot and the bar chart showing CCR4 mRNA expression within total skin cells between BP ( n = 5) and HC ( n = 8). In the box plot b – d : Minima: Lower limit of the whisker. Maxima: Upper limit of the whisker. Center: Median line inside the box. The upper and lower box bounds represent the 25% and 75% percentile of data. e Flow plots of CD3 + T cells from PBMCs showing the expression of CCR4 treated by CCL17 recombinant protein (left panel), and the frequency of CCR4 (right panel) in BP ( n = 30) and HC ( n = 16) groups. f The positive correlation between the level of PLA2G2A and CCL17 in serum from in BP patients ( n = 73). P -value was calculated using two-sided Pearson correlation test. r -value was Pearson correlation coefficient. g The level of CCL17 in PBMC from BP patients ( n = 26) and HC ( n = 7) treated with PLA2G2A recombinant protein. h Effect of CCL17 treatment on the IL-13 secretion from BP patients ( n = 26) and HC ( n = 13). i Effect of PLA2G2A treatment on the IL-13 secretion from BP patients ( n = 20) and HC ( n = 13). j , k ELISA analysis of anti-BP180-NC16A antibody titers in supernatants of CCL17 ( j ) or PLA2G2A ( k ) stimulated PBMCs from BP patients ( n = 26) and HC ( n = 22). P -values in b–d were calculated using two-sided Mann–Whitney U-test. P -values in e and g – k were calculated using paired two-sided Student’s t -test. * P < 0.05, ** P < 0.01, **** P < 0.0001, only P -values < 0.05 are shown. Each sample is represented as one dot.

Article Snippet: The serum levels of IL-13 (EK0424), IL-4 (EK0404), IL-5 (EK0407), PLA2G2A (EK1944), and CCL17 (EK0684) in both patients and controls were quantified using ELISA Development Kits from BosterBio, USA, following the manufacturer’s instructions.

Techniques: Expressing, Enzyme-linked Immunosorbent Assay, Whisker Assay, Recombinant, MANN-WHITNEY

Journal: Nature Communications

Article Title: Single-cell transcriptomics analysis of bullous pemphigoid unveils immune-stromal crosstalk in type 2 inflammatory disease

doi: 10.1038/s41467-024-50283-3

Figure Lengend Snippet: It illustrates a positive feedback loop where fibroblasts respond to Th2 cells through the IL13RA1 - IL13 pair, leading to increased secretion of PLA2G2A and CXCL12. This, in turn, amplifies the Th2-mediated response. (i) The lesional fibroblasts respond to IL-13 and induce the overexpression of PLA2G2A, which promotes the expression of CXCR4 on the surface of immune cells to recruit the immune cells from peripheral blood into skin lesions. (ii) Fibroblasts-derived PLA2G2A and myeloid cells-derived CCL17 elevate the secretion of IL-13, and fibroblast and myeloid cells further respond to IL-13, forming a positive feedback loop between immune cells and fibroblasts. (iii) IL-13 activates B cells to secrete autoantibodies. (iv) In blister, the secretion of autoantibodies recruits T cells, myeloid cells, mast cells, neutrophils and eosinophils. T cell-derived IL-13 activates eosinophils to secrete various cytokines (including IL-13), which further promotes Th2 polarization and mediates the crosstalk between immune cells.

Article Snippet: The serum levels of IL-13 (EK0424), IL-4 (EK0404), IL-5 (EK0407), PLA2G2A (EK1944), and CCL17 (EK0684) in both patients and controls were quantified using ELISA Development Kits from BosterBio, USA, following the manufacturer’s instructions.

Techniques: Over Expression, Expressing, Derivative Assay

Journal:

Article Title: NAD-Dependent DNA-Binding Activity of the Bifunctional NadR Regulator of Salmonella typhimurium

doi:

Figure Lengend Snippet: Strains used

Article Snippet: EK194 , EK193/pLysS; T7 lysozyme under the control of the φ T7 3.8 promoter , Novagen.

Techniques: Expressing, Transformation Assay