Journal: CNS Neuroscience & Therapeutics

Article Title: Vascular and N euronal P rotection I nduced by the O cular A dministration of N erve G rowth F actor in D iabetic‐ I nduced R at E ncephalopathy

doi: 10.1111/cns.12085

Figure Lengend Snippet: (A–D) NGF receptor localization in the septum. The TRKA and p75NTR single stain and co‐stain in the CTR, STZ and STZ+NGF septum are showed in A: note the reduction in cells expressing the NGF receptors in STZ and the recovering effects of oNGF on TRKA/p75NTR cell number and morphology. Magnification shows the different localization of NGF receptors on blood vessels and surrounding area. Specifically, single p75NTR and p75NTR/TRKA co‐stain were found in cells inside and around the blood vessel (B), while only TRKA stains the vasal endothelium and co‐localizes with CD34 (C and D). Bar = 20 μm.

Article Snippet: After blocking, the membranes were incubated overnight at 4°C with primary antibody, such as anti p75NTR, TRKA and proNGF (anti‐rabbit ANT005 purchased by Alomone Labs, Israel).

Techniques: Staining, Expressing

Journal: CNS Neuroscience & Therapeutics

Article Title: Vascular and N euronal P rotection I nduced by the O cular A dministration of N erve G rowth F actor in D iabetic‐ I nduced R at E ncephalopathy

doi: 10.1111/cns.12085

Figure Lengend Snippet: (A–D) Distribution of TRKA and p75NTR in the CA2/3 area of the hippocampus. The effects of diabetes and oNGF on the NGF receptor distribution in CA layers are showed in A. Compared with CTR, TRKA expression is reduced in stratum pyramidalis (SP), and p75NTR immunoreactivity is shifted from the pyramidalis to the stratum oriens (SO) in diabetic rats, while in STZ+NGF rats, an increase in TRKA immunofluorescence is observable in stratum oriens (SO), pyramidale (SP), and lucidum (SL). Cells co‐localizing TRKA and p75NTR are identifiable. TRKA‐positive blood vessels were scarcely found in STZ CA2/3 area but largely increased in STZ+NGF with respect to CTR. The magnification in B and C shows the TRKA‐positive vessels and the p75NTR expression on vessels surrounding cells or intralumen cells. The co‐expression of TRKA and CD34 is showed in Figure 5D. Bar = 20 μm.

Article Snippet: After blocking, the membranes were incubated overnight at 4°C with primary antibody, such as anti p75NTR, TRKA and proNGF (anti‐rabbit ANT005 purchased by Alomone Labs, Israel).

Techniques: Expressing, Immunofluorescence

Journal: CNS Neuroscience & Therapeutics

Article Title: Vascular and N euronal P rotection I nduced by the O cular A dministration of N erve G rowth F actor in D iabetic‐ I nduced R at E ncephalopathy

doi: 10.1111/cns.12085

Figure Lengend Snippet: (A–D) NGF receptors in Dentate gyrus of hippocampus. In A, the effects of diabetes and oNGF on TRKA and p75 expression in DG are shown. Note the increased expression of TRKA on vessels and the TRKA/P75‐positive cells in the hilus of STZ+NGF rats. The white frames indicate the area of magnifications at the bottom panel in which it is possible to observe the different distribution of NGF receptors in the granular layer of the three rat groups. Particulars of p75NTR immunoreactive cells localized closely to TRKA‐stained vessels in STZ+NGF DG are shown in Figure 6B and C. Co‐expression of TRKA and CD34 endothelial marker in vessels is demonstrated in Figure 6D. Bar = 20 μm.

Article Snippet: After blocking, the membranes were incubated overnight at 4°C with primary antibody, such as anti p75NTR, TRKA and proNGF (anti‐rabbit ANT005 purchased by Alomone Labs, Israel).

Techniques: Expressing, Staining, Marker

Journal: CNS Neuroscience & Therapeutics

Article Title: Vascular and N euronal P rotection I nduced by the O cular A dministration of N erve G rowth F actor in D iabetic‐ I nduced R at E ncephalopathy

doi: 10.1111/cns.12085

Figure Lengend Snippet: Expression of TRKA and p75NTR in the septum. The figure on the left shows the representative Western blots for the two NGF receptors. Two bands at 140 and 110 kDa identifying the mature and immature form of TRKA, respectively, were recognized, while a single band was found for p75NTR. Band intensities were normalized to β‐actin, and the receptor expression was quantified from three or four independent experiments, and average levels in septum of CTR, STZ, and STZ+NGF rats are showed in the graphs. *P < 0.0%; **P < 0.01 statistically significant changes.

Article Snippet: After blocking, the membranes were incubated overnight at 4°C with primary antibody, such as anti p75NTR, TRKA and proNGF (anti‐rabbit ANT005 purchased by Alomone Labs, Israel).

Techniques: Expressing, Western Blot

Journal: CNS Neuroscience & Therapeutics

Article Title: Vascular and N euronal P rotection I nduced by the O cular A dministration of N erve G rowth F actor in D iabetic‐ I nduced R at E ncephalopathy

doi: 10.1111/cns.12085

Figure Lengend Snippet: Expression of TRKA and p75NTR in the hippocampus. The effects of diabetes and oNGF on the expression levels of TRKA (110 and 140 kDa), and p75 receptors are visible in the representative Western blot (figure on upper left side), and graphs. *P < 0.05; **P < 0.01 statistically significant changes. Quantifications of three or four independent experiments were performed, and β‐actin expression was used to normalize the sample variability.

Article Snippet: After blocking, the membranes were incubated overnight at 4°C with primary antibody, such as anti p75NTR, TRKA and proNGF (anti‐rabbit ANT005 purchased by Alomone Labs, Israel).

Techniques: Expressing, Western Blot