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Journal: Journal of Immunology Research
Article Title: PM2.5 Causes Increased Bacterial Invasion by Affecting HBD1 Expression in the Lung
doi: 10.1155/2024/6622950
Figure Lengend Snippet: mRNA-seq results of KEGG pathway enrichment analysis and western blot results. (a) mRNA sequencing results of cells exposed to PM2.5, (a1) heat map and (a2) volcano map ( n = 3). (b) KEGG pathway enrichment analysis. Upregulated genes are mainly tumor, MAPK, and NF- κ B pathway-related genes ( n = 3). (c) Signaling pathway related genes change: (c1) MAPK pathway and (c2) NF- κ B pathway ( n = 3). (d) Western blot detected the expression of MAPK pathway and NF- κ B pathway proteins, and the experimental groups were control group, PM2.5 group, PAO1 group, and PAO1 + PM2.5 group. Among them, i κ B and P65 were NF- κ B pathway proteins, P44, P38, and JNK were MAPK pathway proteins, and β -actin and lamin-B were internal reference proteins ( n = 3).
Article Snippet: These membranes were incubated with primary antibodies against i κ B (39 kDa), P65 (65 kDa), P44 (42–44 kDa), P38 (40 kDa),
Techniques: Western Blot, Sequencing, Expressing, Control