Journal: Viruses

Article Title: Evaluation of Trichodysplasia Spinulosa-Associated Polyomavirus Capsid Protein as a New Carrier for Construction of Chimeric Virus-Like Particles Harboring Foreign Epitopes

doi: 10.3390/v7082818

Figure Lengend Snippet: Analysis of T cell activation in spleen cell cultures of mice immunized with TSVP1-PADRE (left panel) and HaVP1-PADRE (right panel) VLPs. Production of cytokines IL-2 (ng/mL) ( A , B ) and IFN-γ (ng/mL) ( C , D ) by murine spleen cells incubated for 24 h with medium alone, HaVP1-PADRE-4 VLPs, unmodified TSPyV VP1 VLPs, unmodified HaPyV VP1VLPs and ConA was determined by ELISA ( A – D ). The number of IFN-γ-secreting cells treated in the same way was analysed by ELISPOT assay ( E , F ). Mean values and SD obtained from each group of immunized mice (indicated on the x axis of each picture) are given (n = 3). The asterisk (*) indicates statistically significant differences between the VLP- or ConA-treated and untreated spleen cell cultures (*, p < 0.05; **, p < 0.01).

Article Snippet: The cells were seeded into sterile 96-well plates for ELISPOT assays (MAIPSWU10, Merck-Millipore) pre-coated overnight with anti-IFN-γ capture antibody (ELISpot development Module Mouse IFN-γ, R&D, MN, USA) in 100 μL of growth medium containing either ConA (3 μg/mL) or VLPs (HaVP1-PADRE-4, TSPyV VP1 and HaPyV VP1) at a concentration 5 μg/mL.

Techniques: Activation Assay, Incubation, Enzyme-linked Immunosorbent Assay, Enzyme-linked Immunospot

Journal: Journal of Clinical Microbiology

Article Title: Human Cytomegalovirus-Specific T-Cell Immune Reconstitution in Preemptively Treated Heart Transplant Recipients Identifies Subjects at Critical Risk for Infection

doi: 10.1128/JCM.06406-11

Figure Lengend Snippet: ELISPOT assay as a predictor of CMV infection. Box-and-whisker plot of CMV-specific IFN-γ ELISPOT levels of HTXs who controlled or experienced CMV viremia within 60 days after ELISPOT determination. The rectangular box connects the upper and lower quartiles and the median. Whiskers connect the upper and lower extremes. The ELISPOT level corresponds to the number of CMV pp65-specific IFN-γ spots/200,000 PBMCs. ELISPOT assay data included those for the test performed from 30 to 360 days posttransplantation. *, P < 0.05.

Article Snippet: PBMCs were resuspended in RPMI 1640 medium supplemented with 10% human type AB serum (Sigma-Aldrich) and seeded at a concentration of 1 × 10 6 cells/ml in 96-well IFN-γ-coated ELISPOT assay plates (Autoimmun Diagnostika).

Techniques: Enzyme-linked Immunospot, Infection, Whisker Assay

Journal: Journal of Clinical Microbiology

Article Title: Human Cytomegalovirus-Specific T-Cell Immune Reconstitution in Preemptively Treated Heart Transplant Recipients Identifies Subjects at Critical Risk for Infection

doi: 10.1128/JCM.06406-11

Figure Lengend Snippet: CMV-specific immunity in 10 pretransplant patients and 48 HTXs. Box-and-whiskers plot of pretransplant (control group), early (<100 days posttransplant), and late (>100 days posttransplant) CMV-specific IFN-γ ELISPOT levels in HTXs. Data are for 19 HTXs with single ELISPOT assay determinations taken <100 or >100 days after transplant and ELISPOT assay and 29 HTXs followed prospectively both before and after 100 days after transplant. Numbers in parentheses display the number of ELISPOT assay determinations done for each group.

Article Snippet: PBMCs were resuspended in RPMI 1640 medium supplemented with 10% human type AB serum (Sigma-Aldrich) and seeded at a concentration of 1 × 10 6 cells/ml in 96-well IFN-γ-coated ELISPOT assay plates (Autoimmun Diagnostika).

Techniques: Enzyme-linked Immunospot

Journal: Journal of Clinical Microbiology

Article Title: Human Cytomegalovirus-Specific T-Cell Immune Reconstitution in Preemptively Treated Heart Transplant Recipients Identifies Subjects at Critical Risk for Infection

doi: 10.1128/JCM.06406-11

Figure Lengend Snippet: Immune reconstitution of 29 HTXs followed prospectively before and after 100 days after transplant. Dotted lines show HTX IFN-γ ELISPOT levels before and after 100 days posttransplant. Arrows indicate the CMV-specific immune response of one HTX who experienced CMV disease. For the other patient experiencing CMV disease, we did not have data from sequential time points.

Article Snippet: PBMCs were resuspended in RPMI 1640 medium supplemented with 10% human type AB serum (Sigma-Aldrich) and seeded at a concentration of 1 × 10 6 cells/ml in 96-well IFN-γ-coated ELISPOT assay plates (Autoimmun Diagnostika).

Techniques: Enzyme-linked Immunospot