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Image Search Results
Journal: Journal of Virology
Article Title: Hepatitis C Virus Infection Induces Apoptosis through a Bax-Triggered, Mitochondrion-Mediated, Caspase 3-Dependent Pathway
doi: 10.1128/jvi.00395-08
Figure Lengend Snippet: FIG. 8. HCV infection does not induce ER stress in Huh7.5 cells. Huh7.5 cells infected with HCV and mock-infected controls were harvested at 2, 4, and 6 days postinfection (dpi), and the whole-cell lysates were subjected to immunoblot analysis using antibodies against GRP78, GRP94, NS3, and actin. Amounts of actin were measured to verify equal amounts of sample loading. Huh7.5 cells treated with tunicamycin (TM; 5 g/ml) for 48 h served as a positive control.
Article Snippet: The primary antibodies used were mouse monoclonal antibodies against cytochrome c (A-8; Santa Cruz Biotechnology, Santa Cruz, CA), HCV NS3 (Chemicon), Tim23, Bax and Bcl-2 (BD Biosciences Pharmingen, San Diego, CA); rabbit polyclonal antibodies against Bak (Upstate), caspase 3, and PARP (Cell Signaling Technology, Danvers, MA); and goat polyclonal antibodies against glucose-regulated protein 78 (GRP78) and
Techniques: Infection, Western Blot, Positive Control