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Cell Signaling Technology Inc pstat1
TLR7-induced phosphorylation of MAPKs and STAT1 is regulated by TREML4. ( a-d ) Phosphoflow cytometry analysis of p44p42 ( a ), p38 ( b ), <t>pSTAT1(S727)</t> ( c ) and <t>pSTAT1(Y701)</t> ( d ) in CD11c + BMDCs isolated from Treml4 +/+ and Treml4 –/– mice treated with 100 ng/mL LPS, 10 μg/mL GRD, or 10 μg/mL CpG-DNA for 30 min. Histograms are representative of one experiment of 3 independent experiments performed. Graphs represent the mean fluorescent intensity (MFI). * P <0.01 (Student's t -test; error bars, mean and s.d.).
Pstat1, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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TLR7-induced phosphorylation of MAPKs and STAT1 is regulated by TREML4. ( a-d ) Phosphoflow cytometry analysis of p44p42 ( a ), p38 ( b ), pSTAT1(S727) ( c ) and pSTAT1(Y701) ( d ) in CD11c + BMDCs isolated from Treml4 +/+ and Treml4 –/– mice treated with 100 ng/mL LPS, 10 μg/mL GRD, or 10 μg/mL CpG-DNA for 30 min. Histograms are representative of one experiment of 3 independent experiments performed. Graphs represent the mean fluorescent intensity (MFI). * P <0.01 (Student's t -test; error bars, mean and s.d.).

Journal: Nature immunology

Article Title: TREML4 amplifies TLR7-mediated signaling during antiviral responses and autoimmunity

doi: 10.1038/ni.3143

Figure Lengend Snippet: TLR7-induced phosphorylation of MAPKs and STAT1 is regulated by TREML4. ( a-d ) Phosphoflow cytometry analysis of p44p42 ( a ), p38 ( b ), pSTAT1(S727) ( c ) and pSTAT1(Y701) ( d ) in CD11c + BMDCs isolated from Treml4 +/+ and Treml4 –/– mice treated with 100 ng/mL LPS, 10 μg/mL GRD, or 10 μg/mL CpG-DNA for 30 min. Histograms are representative of one experiment of 3 independent experiments performed. Graphs represent the mean fluorescent intensity (MFI). * P <0.01 (Student's t -test; error bars, mean and s.d.).

Article Snippet: Cells were washed twice to remove methanol and stained with fluorescently labeled antibodies CD11c-APC, p42/44-Alexa488 (Y202/204; Cell Signaling), p38MAPK-PE (T180/Y182; Cell Signaling), pSTAT1 (S727; Cell Signaling clone D3B7), pSTAT1 (Y701; Cell Signaling clone 58D6).

Techniques: Phospho-proteomics, Cytometry, Isolation