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Antibodies Used in This Study
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Antibodies Used in This Study

Journal: Journal of Neuropathology and Experimental Neurology

Article Title: Slow Progressive Accumulation of Oligodendroglial Alpha-Synuclein (α-Syn) Pathology in Synthetic α-Syn Fibril-Induced Mouse Models of Synucleinopathy

doi: 10.1093/jnen/nlz070

Figure Lengend Snippet: Antibodies Used in This Study

Article Snippet: Antibody Epitope Host Species Dilution Source 81A α-Syn phosphorylated at Ser 129 Mouse monoclonal 1:20 000 (IHC), 1:5000 (IF) ( 30 ) #64 α-Syn phosphorylated at Ser 129 Mouse monoclonal 1:5000 (IHC), 1:1000 (IF) Wako (Saitama, Japan, #015-25191) EP1536Y α-Syn phosphorylated at Ser 129 Rabbit monoclonal 1:20 000 (IHC), 1:5000 (IF) Abcam (Cambridge, MA, #ab51253) LB509 Human α-Syn Mouse monoclonal 1:1000 (IHC) Invitrogen (Carlsbad, CA, #180215) Syn506 Misfolded α-Syn Mouse monoclonal 1:5000 (IHC) ( 31 ) Syn514 Misfolded α-Syn Mouse monoclonal 1:100 (IHC) ( 31 ) NeuN Neuronal nuclear antigen Mouse monoclonal 1:200 (IF) Millipore (Burlington, MA, #MAB377) GFAP Glial fibrillary acidic protein Mouse monoclonal 1:500 (IF) Sigma (St. Louis, MO, #G3893) Iba1 Ionized calcium-binding adapter molecule 1 Rabbit polyclonal 1:500 (IF) Wako (#019-19741) Olig2 Oligodendrocyte transcription factor 2 Rabbit polyclonal 1:500 (IF) Millipore (#AB9610) GST-pi Glutathione S-transferase pi Rabbit polyclonal 1:500 (IF) MBL (Woburn, MA, #312) PDGFR-α Platelet-derived growth factor receptor-α Rabbit monoclonal 1:200 (IF) Cell Signaling (Danvers, MA, #3174S) p62 Sequestosome 1 (p62/SQSTM1) Rabbit polyclonal 1:1000 (IF) MBL (#P045) Ubiquitin Ubiquitin Rabbit polyclonal 1:500 (IF) DAKO (Carpinteria, CA, #Z0458) TPPP/p25 Tubulin polymerization promoting protein Rabbit monoclonal 1:200 (IF) Abcam (#ab92305) pNF-H Phosphorylated neurofilament heavy chain (pNF-H) Mouse monoclonal 1:1000 (IF) COVANCE (Princeton, NJ, #SMI31P) MAP2 Microtubule-associated protein 2 Mouse monoclonal 1:200 (IF) Millipore (#MAB3418) Open in a separate window Description of all antibodies used in this study, including epitopes they recognize, concentrations used, and how they were procured.

Techniques: