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Image Search Results
Journal: Biomolecules & Therapeutics
Article Title: Adipose-Derived Stem Cell Coculturing Stimulates Integrin-Mediated Extracellular Matrix Adhesion of Melanocytes by Upregulating Growth Factors
doi: 10.4062/biomolther.2018.203
Figure Lengend Snippet: ADSC coculturing enhanced melanocyte adhesion to ECM through β1 integrin upregulation. (A) Western blot analysis for relative levels of integrins β1, α5, and α6 proteins. α-tubulin was used as an internal control. (B) Representative immunofluorescent staining using anti-integrin β1, anti-integrin α5, or anti-integrin α6 antibody. Nuclei were count-stained with Hoechst 33258 (Bar=0.05 mm) and intensities were measured using Wright Cell Imaging Facility ImageJ software. (C, D) Adhesion assay on fibronectin- (C) or laminin-coated culture dishes (D) treated with or without anti-integrin β1 antibody. Nuclei were stained with Hoechst 33258 (Bar=0.2 mm). These studies were done using melanocyte monocultures (monoMC) and melanocytes taken from upper insert after coculturing with ADSCs in lower chamber (coMC). Data in each graph represent mean ± SD of three independent experiments. * p <0.05 vs monoMC, # p <0.05 vs MC without anti-integrin β1 antibody.
Article Snippet: These membranes were incubated with antibodies specific for bFGF (mouse monoclonal, BD Biosciences, San Jose, CA, USA), SCF (mouse monoclonal, Santa Cruz Biotechnology Inc., CA, USA),
Techniques: Western Blot, Control, Staining, Imaging, Software, Cell Adhesion Assay
Journal: Biomolecules & Therapeutics
Article Title: Adipose-Derived Stem Cell Coculturing Stimulates Integrin-Mediated Extracellular Matrix Adhesion of Melanocytes by Upregulating Growth Factors
doi: 10.4062/biomolther.2018.203
Figure Lengend Snippet: Upregulation of bFGF and SCF by ADSCs was involved in integrin-mediated melanocyte adhesion to ECM. (A, B) Western blot analysis for relative levels of integrins β1, α5, and α6 proteins in melanocytes treated with or without anti-bFGF (A) or anti-SCF antibody (B). (C, D) Adhesion assay using fibronectin- (C) or laminin-coated culture dishes (D) treated with or without anti-bFGF or anti-SCF antibody. Nuclei were stained with Hoechst 33258 (Bar=0.2 mm). These experiments were performed using melanocyte monocultures (monoMC) and melanocytes taken from upper insert after coculturing with ADSCs in lower chamber (coMC). Data in each graph represent mean ± SD of three independent experiments. * p <0.05 vs monoMC, # p <0.05 vs coMC.
Article Snippet: These membranes were incubated with antibodies specific for bFGF (mouse monoclonal, BD Biosciences, San Jose, CA, USA), SCF (mouse monoclonal, Santa Cruz Biotechnology Inc., CA, USA),
Techniques: Western Blot, Cell Adhesion Assay, Staining
Journal: Biomolecules & Therapeutics
Article Title: Adipose-Derived Stem Cell Coculturing Stimulates Integrin-Mediated Extracellular Matrix Adhesion of Melanocytes by Upregulating Growth Factors
doi: 10.4062/biomolther.2018.203
Figure Lengend Snippet: Grafting of melanocyte-ADSC cocultures increased levels of growth factors and integrins in nude mice. Representative immunofluorescent staining using anti-bFGF or anti-SCF (A) and anti-integrin β1, anti-integrin α5, or anti-integrin α6 (B) in skin specimens of nude mice grafted with ADSC monocultures (ADSC), melanocyte monocultures (MC), or melanocyte+ADSC cocultures (MC+ADSC). Nuclei were count-stained with Hoechst 33258 (Bar=0.05 mm). Intensities were measured in five randomly selected high-power fields (x400) of the dermis using Wright Cell Imaging Facility ImageJ software. Data in each graph represent mean ± SD from five nude mice. * p <0.05 vs ADSC.
Article Snippet: These membranes were incubated with antibodies specific for bFGF (mouse monoclonal, BD Biosciences, San Jose, CA, USA), SCF (mouse monoclonal, Santa Cruz Biotechnology Inc., CA, USA),
Techniques: Staining, Imaging, Software