735a Search Results


92
TargetMol sts
Sts, supplied by TargetMol, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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93
Bethyl rabbit orc2 antibody
Rabbit Orc2 Antibody, supplied by Bethyl, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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92
Bethyl integrin β1
ADSC coculturing enhanced melanocyte adhesion to ECM through <t>β1</t> <t>integrin</t> upregulation. (A) Western blot analysis for relative levels of integrins β1, α5, and α6 proteins. α-tubulin was used as an internal control. (B) Representative immunofluorescent staining using <t>anti-integrin</t> <t>β1,</t> anti-integrin α5, or anti-integrin α6 antibody. Nuclei were count-stained with Hoechst 33258 (Bar=0.05 mm) and intensities were measured using Wright Cell Imaging Facility ImageJ software. (C, D) Adhesion assay on fibronectin- (C) or laminin-coated culture dishes (D) treated with or without anti-integrin β1 antibody. Nuclei were stained with Hoechst 33258 (Bar=0.2 mm). These studies were done using melanocyte monocultures (monoMC) and melanocytes taken from upper insert after coculturing with ADSCs in lower chamber (coMC). Data in each graph represent mean ± SD of three independent experiments. * p <0.05 vs monoMC, # p <0.05 vs MC without anti-integrin β1 antibody.
Integrin β1, supplied by Bethyl, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 92 stars, based on 1 article reviews
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91
Bethyl anti strap antibody
ADSC coculturing enhanced melanocyte adhesion to ECM through <t>β1</t> <t>integrin</t> upregulation. (A) Western blot analysis for relative levels of integrins β1, α5, and α6 proteins. α-tubulin was used as an internal control. (B) Representative immunofluorescent staining using <t>anti-integrin</t> <t>β1,</t> anti-integrin α5, or anti-integrin α6 antibody. Nuclei were count-stained with Hoechst 33258 (Bar=0.05 mm) and intensities were measured using Wright Cell Imaging Facility ImageJ software. (C, D) Adhesion assay on fibronectin- (C) or laminin-coated culture dishes (D) treated with or without anti-integrin β1 antibody. Nuclei were stained with Hoechst 33258 (Bar=0.2 mm). These studies were done using melanocyte monocultures (monoMC) and melanocytes taken from upper insert after coculturing with ADSCs in lower chamber (coMC). Data in each graph represent mean ± SD of three independent experiments. * p <0.05 vs monoMC, # p <0.05 vs MC without anti-integrin β1 antibody.
Anti Strap Antibody, supplied by Bethyl, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 91 stars, based on 1 article reviews
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93
Bethyl anti gsdmd
ADSC coculturing enhanced melanocyte adhesion to ECM through <t>β1</t> <t>integrin</t> upregulation. (A) Western blot analysis for relative levels of integrins β1, α5, and α6 proteins. α-tubulin was used as an internal control. (B) Representative immunofluorescent staining using <t>anti-integrin</t> <t>β1,</t> anti-integrin α5, or anti-integrin α6 antibody. Nuclei were count-stained with Hoechst 33258 (Bar=0.05 mm) and intensities were measured using Wright Cell Imaging Facility ImageJ software. (C, D) Adhesion assay on fibronectin- (C) or laminin-coated culture dishes (D) treated with or without anti-integrin β1 antibody. Nuclei were stained with Hoechst 33258 (Bar=0.2 mm). These studies were done using melanocyte monocultures (monoMC) and melanocytes taken from upper insert after coculturing with ADSCs in lower chamber (coMC). Data in each graph represent mean ± SD of three independent experiments. * p <0.05 vs monoMC, # p <0.05 vs MC without anti-integrin β1 antibody.
Anti Gsdmd, supplied by Bethyl, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 93 stars, based on 1 article reviews
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90
METTLER TOLEDO mettler 735a
ADSC coculturing enhanced melanocyte adhesion to ECM through <t>β1</t> <t>integrin</t> upregulation. (A) Western blot analysis for relative levels of integrins β1, α5, and α6 proteins. α-tubulin was used as an internal control. (B) Representative immunofluorescent staining using <t>anti-integrin</t> <t>β1,</t> anti-integrin α5, or anti-integrin α6 antibody. Nuclei were count-stained with Hoechst 33258 (Bar=0.05 mm) and intensities were measured using Wright Cell Imaging Facility ImageJ software. (C, D) Adhesion assay on fibronectin- (C) or laminin-coated culture dishes (D) treated with or without anti-integrin β1 antibody. Nuclei were stained with Hoechst 33258 (Bar=0.2 mm). These studies were done using melanocyte monocultures (monoMC) and melanocytes taken from upper insert after coculturing with ADSCs in lower chamber (coMC). Data in each graph represent mean ± SD of three independent experiments. * p <0.05 vs monoMC, # p <0.05 vs MC without anti-integrin β1 antibody.
Mettler 735a, supplied by METTLER TOLEDO, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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90
Rohm and Haas adhesive adcotetm 735a-75/c2
ADSC coculturing enhanced melanocyte adhesion to ECM through <t>β1</t> <t>integrin</t> upregulation. (A) Western blot analysis for relative levels of integrins β1, α5, and α6 proteins. α-tubulin was used as an internal control. (B) Representative immunofluorescent staining using <t>anti-integrin</t> <t>β1,</t> anti-integrin α5, or anti-integrin α6 antibody. Nuclei were count-stained with Hoechst 33258 (Bar=0.05 mm) and intensities were measured using Wright Cell Imaging Facility ImageJ software. (C, D) Adhesion assay on fibronectin- (C) or laminin-coated culture dishes (D) treated with or without anti-integrin β1 antibody. Nuclei were stained with Hoechst 33258 (Bar=0.2 mm). These studies were done using melanocyte monocultures (monoMC) and melanocytes taken from upper insert after coculturing with ADSCs in lower chamber (coMC). Data in each graph represent mean ± SD of three independent experiments. * p <0.05 vs monoMC, # p <0.05 vs MC without anti-integrin β1 antibody.
Adhesive Adcotetm 735a 75/C2, supplied by Rohm and Haas, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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90
Biologica Environmental Services msnm am 735 a-b
ADSC coculturing enhanced melanocyte adhesion to ECM through <t>β1</t> <t>integrin</t> upregulation. (A) Western blot analysis for relative levels of integrins β1, α5, and α6 proteins. α-tubulin was used as an internal control. (B) Representative immunofluorescent staining using <t>anti-integrin</t> <t>β1,</t> anti-integrin α5, or anti-integrin α6 antibody. Nuclei were count-stained with Hoechst 33258 (Bar=0.05 mm) and intensities were measured using Wright Cell Imaging Facility ImageJ software. (C, D) Adhesion assay on fibronectin- (C) or laminin-coated culture dishes (D) treated with or without anti-integrin β1 antibody. Nuclei were stained with Hoechst 33258 (Bar=0.2 mm). These studies were done using melanocyte monocultures (monoMC) and melanocytes taken from upper insert after coculturing with ADSCs in lower chamber (coMC). Data in each graph represent mean ± SD of three independent experiments. * p <0.05 vs monoMC, # p <0.05 vs MC without anti-integrin β1 antibody.
Msnm Am 735 A B, supplied by Biologica Environmental Services, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
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Image Search Results


ADSC coculturing enhanced melanocyte adhesion to ECM through β1 integrin upregulation. (A) Western blot analysis for relative levels of integrins β1, α5, and α6 proteins. α-tubulin was used as an internal control. (B) Representative immunofluorescent staining using anti-integrin β1, anti-integrin α5, or anti-integrin α6 antibody. Nuclei were count-stained with Hoechst 33258 (Bar=0.05 mm) and intensities were measured using Wright Cell Imaging Facility ImageJ software. (C, D) Adhesion assay on fibronectin- (C) or laminin-coated culture dishes (D) treated with or without anti-integrin β1 antibody. Nuclei were stained with Hoechst 33258 (Bar=0.2 mm). These studies were done using melanocyte monocultures (monoMC) and melanocytes taken from upper insert after coculturing with ADSCs in lower chamber (coMC). Data in each graph represent mean ± SD of three independent experiments. * p <0.05 vs monoMC, # p <0.05 vs MC without anti-integrin β1 antibody.

Journal: Biomolecules & Therapeutics

Article Title: Adipose-Derived Stem Cell Coculturing Stimulates Integrin-Mediated Extracellular Matrix Adhesion of Melanocytes by Upregulating Growth Factors

doi: 10.4062/biomolther.2018.203

Figure Lengend Snippet: ADSC coculturing enhanced melanocyte adhesion to ECM through β1 integrin upregulation. (A) Western blot analysis for relative levels of integrins β1, α5, and α6 proteins. α-tubulin was used as an internal control. (B) Representative immunofluorescent staining using anti-integrin β1, anti-integrin α5, or anti-integrin α6 antibody. Nuclei were count-stained with Hoechst 33258 (Bar=0.05 mm) and intensities were measured using Wright Cell Imaging Facility ImageJ software. (C, D) Adhesion assay on fibronectin- (C) or laminin-coated culture dishes (D) treated with or without anti-integrin β1 antibody. Nuclei were stained with Hoechst 33258 (Bar=0.2 mm). These studies were done using melanocyte monocultures (monoMC) and melanocytes taken from upper insert after coculturing with ADSCs in lower chamber (coMC). Data in each graph represent mean ± SD of three independent experiments. * p <0.05 vs monoMC, # p <0.05 vs MC without anti-integrin β1 antibody.

Article Snippet: These membranes were incubated with antibodies specific for bFGF (mouse monoclonal, BD Biosciences, San Jose, CA, USA), SCF (mouse monoclonal, Santa Cruz Biotechnology Inc., CA, USA), integrin β1 and integrin α6 (rabbit polyclonal, Bethyl laboratories Inc., TX, USA), and integrin α5 and α-tubulin (mouse monoclonal, Santa Cruz Biotechnology Inc.).

Techniques: Western Blot, Control, Staining, Imaging, Software, Cell Adhesion Assay

Upregulation of bFGF and SCF by ADSCs was involved in integrin-mediated melanocyte adhesion to ECM. (A, B) Western blot analysis for relative levels of integrins β1, α5, and α6 proteins in melanocytes treated with or without anti-bFGF (A) or anti-SCF antibody (B). (C, D) Adhesion assay using fibronectin- (C) or laminin-coated culture dishes (D) treated with or without anti-bFGF or anti-SCF antibody. Nuclei were stained with Hoechst 33258 (Bar=0.2 mm). These experiments were performed using melanocyte monocultures (monoMC) and melanocytes taken from upper insert after coculturing with ADSCs in lower chamber (coMC). Data in each graph represent mean ± SD of three independent experiments. * p <0.05 vs monoMC, # p <0.05 vs coMC.

Journal: Biomolecules & Therapeutics

Article Title: Adipose-Derived Stem Cell Coculturing Stimulates Integrin-Mediated Extracellular Matrix Adhesion of Melanocytes by Upregulating Growth Factors

doi: 10.4062/biomolther.2018.203

Figure Lengend Snippet: Upregulation of bFGF and SCF by ADSCs was involved in integrin-mediated melanocyte adhesion to ECM. (A, B) Western blot analysis for relative levels of integrins β1, α5, and α6 proteins in melanocytes treated with or without anti-bFGF (A) or anti-SCF antibody (B). (C, D) Adhesion assay using fibronectin- (C) or laminin-coated culture dishes (D) treated with or without anti-bFGF or anti-SCF antibody. Nuclei were stained with Hoechst 33258 (Bar=0.2 mm). These experiments were performed using melanocyte monocultures (monoMC) and melanocytes taken from upper insert after coculturing with ADSCs in lower chamber (coMC). Data in each graph represent mean ± SD of three independent experiments. * p <0.05 vs monoMC, # p <0.05 vs coMC.

Article Snippet: These membranes were incubated with antibodies specific for bFGF (mouse monoclonal, BD Biosciences, San Jose, CA, USA), SCF (mouse monoclonal, Santa Cruz Biotechnology Inc., CA, USA), integrin β1 and integrin α6 (rabbit polyclonal, Bethyl laboratories Inc., TX, USA), and integrin α5 and α-tubulin (mouse monoclonal, Santa Cruz Biotechnology Inc.).

Techniques: Western Blot, Cell Adhesion Assay, Staining

Grafting of melanocyte-ADSC cocultures increased levels of growth factors and integrins in nude mice. Representative immunofluorescent staining using anti-bFGF or anti-SCF (A) and anti-integrin β1, anti-integrin α5, or anti-integrin α6 (B) in skin specimens of nude mice grafted with ADSC monocultures (ADSC), melanocyte monocultures (MC), or melanocyte+ADSC cocultures (MC+ADSC). Nuclei were count-stained with Hoechst 33258 (Bar=0.05 mm). Intensities were measured in five randomly selected high-power fields (x400) of the dermis using Wright Cell Imaging Facility ImageJ software. Data in each graph represent mean ± SD from five nude mice. * p <0.05 vs ADSC.

Journal: Biomolecules & Therapeutics

Article Title: Adipose-Derived Stem Cell Coculturing Stimulates Integrin-Mediated Extracellular Matrix Adhesion of Melanocytes by Upregulating Growth Factors

doi: 10.4062/biomolther.2018.203

Figure Lengend Snippet: Grafting of melanocyte-ADSC cocultures increased levels of growth factors and integrins in nude mice. Representative immunofluorescent staining using anti-bFGF or anti-SCF (A) and anti-integrin β1, anti-integrin α5, or anti-integrin α6 (B) in skin specimens of nude mice grafted with ADSC monocultures (ADSC), melanocyte monocultures (MC), or melanocyte+ADSC cocultures (MC+ADSC). Nuclei were count-stained with Hoechst 33258 (Bar=0.05 mm). Intensities were measured in five randomly selected high-power fields (x400) of the dermis using Wright Cell Imaging Facility ImageJ software. Data in each graph represent mean ± SD from five nude mice. * p <0.05 vs ADSC.

Article Snippet: These membranes were incubated with antibodies specific for bFGF (mouse monoclonal, BD Biosciences, San Jose, CA, USA), SCF (mouse monoclonal, Santa Cruz Biotechnology Inc., CA, USA), integrin β1 and integrin α6 (rabbit polyclonal, Bethyl laboratories Inc., TX, USA), and integrin α5 and α-tubulin (mouse monoclonal, Santa Cruz Biotechnology Inc.).

Techniques: Staining, Imaging, Software