Journal: Nature Communications

Article Title: Early-life gut mycobiome core species modulate metabolic health in mice

doi: 10.1038/s41467-025-56743-8

Figure Lengend Snippet: A Gnotobiotic colonization and diet-induced obesity model. Germ-free dams were gavaged twice with the Oligo-MM12 consortium alone (B; gray) or in combination with C. albicans (B + C; orange), R. mucilaginosa (B + R; pink) or M. restricta (B + M; purple; Males—SD: n B = 23, n B+C = 8, n B+R = 10, n B+M = 12; Females—SD: n B = 18, n B+C = 8, n B+R = 8, n B+M = 12; Males—HFHS: n B = 22, n B+C = 10, n B+R = 8, n B+M = 12; Females—HFHS: n B = 20, n B+C = 12, n B+R = 7, n B+M = 10). Twice in the first week of life, offspring (F1) were exposed to the fungal species corresponding to their colonization group. F1 were weaned onto standard diet (SD) or high-fat-high-sucrose diet (HFHS) until 12 weeks of age. Graphics from BioRender.com. https://BioRender.com/y96j811 . B Fungal colony count in fecal samples at 6–7 weeks of age. One mouse sampled per cage (SD: n B+C = 6, n B+R = 8; HFHS: n B+C = 6, n B+R = 7). Two-way ANOVA with Sidak’s multiple comparisons test. * p < 0.05, ** p < 0.01, *** p < 0.001. C , D Fecal ITS DNA at 12 weeks in males and females, respectively (Male—SD: n B = 13, n B+C = 7, n B+R = 7, n B+M = 8; Male—HFHS: n B = 16, n B+C = 8, n B+R = 8, n B+M = 8; Female—SD: n B = 14, n B+C = 8, n B+R = 8, n B+M = 8; Female—HFHS: n B = 14, n B+C = 8, n B+R = 6, n B+M = 8). Data represented as mean ± SEM; n indicates number of mice sampled.

Article Snippet: These consortia included (1) the Oligo-MM12 alone “B” ( A. muris KB18, A. muciniphila YL44, B. caecimuris I48, B. animalis YL2, B. pseudococcoides YL58, C. innocuum I46, E. clostridioforme YL32, E. faecalis KB1, F. plautii YL31, L. reuteri I49, M. intestinale YL27 , and T. muris YL45 ) or (2) in combination with C. albicans (This strain was isolated from infant stool samples enrolled in a clinical study at the Alberta Children’s Hospital in Calgary, Canada ) “B + C”, (3) R. mucilaginosa (DSMZ; DSM 70825) “B + R”, or (4) M. restri cta (ATCC; MYA4611) “B + M”.

Techniques:

Journal: Kidney international

Article Title: Streptococcal pyrogenic exotoxin B antibodies in a mouse model of glomerulonephritis.

doi: 10.1038/sj.ki.5002407

Figure Lengend Snippet: Figure 4 | Complement activation in the kidney of mice hyperimmunized with 28-kDa SPE B mutant protein C192S. BALB/c mice were immunized four times with (a) BSA or (b) C192S. Complement C3 deposits were detected using FITC-conjugated anti-C3d antibody with fluorescence microscopy (n ¼ 10 per group). (c) The mean integral fluorescent intensity of glomeruli was analyzed in 10 fields per mouse (n ¼ 10 mice). Original magnification 200.

Article Snippet: Complement C3 was detected using sheep anti-human C3d (Serotec Ltd), which cross-reacts with mouse C3d, and then using FITC-conjugated goat anti-sheep IgG antibody (Jackson ImmunoResearch Laboratories Inc.).

Techniques: Activation Assay, Mutagenesis, Fluorescence, Microscopy

Journal: Kidney international

Article Title: Streptococcal pyrogenic exotoxin B antibodies in a mouse model of glomerulonephritis.

doi: 10.1038/sj.ki.5002407

Figure Lengend Snippet: Figure 8 | Immunoglobulin deposition and complement activation in mice after passive immunization with monoclonal anti-SPE B antibody 10G. BALB/c mice were intravenously immunized each day with 500 mg of monoclonal 9C or 10G IgG for 3 days and, after 7 days, the kidney sections were analyzed using (a, c) FITC-conjugated anti-mouse IgG or (b, d) anti-C3d antibody (n ¼ 3 per group). Original magnification 200.

Article Snippet: Complement C3 was detected using sheep anti-human C3d (Serotec Ltd), which cross-reacts with mouse C3d, and then using FITC-conjugated goat anti-sheep IgG antibody (Jackson ImmunoResearch Laboratories Inc.).

Techniques: Activation Assay