Journal: medRxiv

Article Title: Recovery from Heart Failure is a Vascular Recovery

doi: 10.1101/2024.07.24.24310960

Figure Lengend Snippet: CD31 co-staining with fluorescent microsphere beads. The microsphere beads (blue) and CD31 (red) staining are co-localized (white arrows) suggesting that beads fill up the capillaries and are not leaking out of the vessels.

Article Snippet: Additionally, CD31 mouse (1:200, NBP2-34283, Novus Biologicals) and AF647 donkey anti-mouse (1:500, A-31571, Invitrogen) staining was performed on some tissue sections to confirm that beads filled the small capillaries without leaking out of the vessels. ( ).

Techniques: Staining

Journal: medRxiv

Article Title: Recovery from Heart Failure is a Vascular Recovery

doi: 10.1101/2024.07.24.24310960

Figure Lengend Snippet: Angiogenic transdifferentiation during heart failure recovery in Col1a2-CreERT:R26R tdTomato mice. a , Schematic of fibroblast lineage tracing in the HFR mouse model. Col1a2-expressing fibroblast cells and their progenies are marked with tdTomato after tamoxifen (4-OHT) induction. b, IF staining visualized by confocal imaging in HFR group showed CD31+ (green) tdTomato+ (red) dual staining (yellow) cells representing endothelial cells transdifferentiated from fibroblasts (arrows - dual staining cells). c, The 3-side view by confocal microscope confirmed the dual staining of the cells that expressed both CD31 and tdTomato. d, Angiogenic transdifferentiation during HFR quantified by flow cytometry. e, HFR group shows increased number of transdifferentiated (CD31+ tdTomato+) cells. Control-Non-operated tamoxifen induced age-matched mice; HFR-Heart failure recovery. (n=5 mice/group, p value-****<0.0001, using an unpaired t-test, Graph shows mean ± S.E.M)

Article Snippet: Additionally, CD31 mouse (1:200, NBP2-34283, Novus Biologicals) and AF647 donkey anti-mouse (1:500, A-31571, Invitrogen) staining was performed on some tissue sections to confirm that beads filled the small capillaries without leaking out of the vessels. ( ).

Techniques: Expressing, Staining, Imaging, Microscopy, Flow Cytometry, Control

Journal: medRxiv

Article Title: Recovery from Heart Failure is a Vascular Recovery

doi: 10.1101/2024.07.24.24310960

Figure Lengend Snippet: Gating strategy for tdTomato+ cells from CD31+ cells for flow cytometry. Live cells were gated based on DAPI and CD45 positive immune cells which were excluded. CD31 positive endothelial cells were selected and stained for tdTomato and the positive cells were deemed as the transdifferentiation population.

Article Snippet: Additionally, CD31 mouse (1:200, NBP2-34283, Novus Biologicals) and AF647 donkey anti-mouse (1:500, A-31571, Invitrogen) staining was performed on some tissue sections to confirm that beads filled the small capillaries without leaking out of the vessels. ( ).

Techniques: Flow Cytometry, Staining

Journal: Stem cell research & therapy

Article Title: miR-126-3p-loaded small extracellular vesicles secreted by urine-derived stem cells released from a phototriggered imine crosslink hydrogel could enhance vaginal epithelization after vaginoplasty.

doi: 10.1186/s13287-022-03003-x

Figure Lengend Snippet: Fig. 4 piGEL-sEVs promoted vaginal epithelium regeneration and angiogenesis. A IF staining for AE1/AE3 at days 7 and 14 after the operation. Scale bar: 50 μm. B Quantitative analysis of IF staining of AE1/AE3. n = 3 per group. C IF staining for CD31 at day 7 after the operation. Scale bar: 50 μm. D Quantitative analysis of new vessel number. n = 3 per group. E IF staining for α-SMA at day 14 after the operation. Scale bar: 50 μm. F Quantitative analysis of IF staining of α-SMA. n = 3 per group. *, P < 0.05, compared with the PBS group; #, P < 0.05, compared with the piGEL group; %, P < 0.05, compared with the sEVs group

Article Snippet: The collected samples were fixed in 4% PFA, dehydrated with gradient series of sucrose and embedded in OCT. Ten-micrometer thick sections were incubated with primary antibodies, including those targeting AE1/AE3 (1:100; Thermo Fisher, USA, 53-9003-82), CD31 (1:100; Novus, Germany, JC/70A), and α-SMA (1:200; Abcam, UK, ab7817).

Techniques: Staining