Journal: Biomedical Journal of Scientific & Technical Research

Article Title: "Age-Related Variations of the Brain Mitochondrial Permeability Transition Pore and Complex I in Response to Hypoxia"

doi: 10.26717/bjstr.2023.51.008169

Figure Lengend Snippet: Figure 1: Protein levels of CypD and NDUFS2 relative to VDAC1. (a) Representative blots and quantification of relative CypD levels (n=4). (b) Representative blots and quantification of relative NDUFS2 levels (n=5). *- statistically significantly different compared to 24–26 months group.

Article Snippet: The primary antibodies were: NDUFS2 antibody (NBP230413, Novus biologicals) diluted 1:1000 in blocking buffer (5% BSA in TBST); Cyclophilin-F Antibody (JM71-39, Novus biologicals) diluted 1:2000 in blocking buffer; and VDAC1 Antibody (SA93-03, Novus biologicals) diluted 1:2000 in blocking buffer.

Techniques:

Journal: The Journal of Experimental Medicine

Article Title: Superior antigen cross-presentation and XCR1 expression define human CD11c + CD141 + cells as homologues of mouse CD8 + dendritic cells

doi: 10.1084/jem.20100348

Figure Lengend Snippet: XCL1 induces a [Ca 2+ ] i signal in CD141 + DCs. CD141 + DCs were flow sorted to a purity >98.7%, immobilized on poly– l -lysine–coated glass coverslips, and loaded with 2 µM fura-2/AM. Cells were imaged in a monochromator-assisted digital video imaging system and challenged with 1 µg/ml XCL1 as indicated (left arrow). Subsequently, the same cells were challenged again with a mixture of 100 ng/ml CCL2, 200 ng/ml CCL21, 200 ng/ml CXCL9, and 1 ng/ml CX3CL1 used as a positive control (right arrow). The data shown represent [Ca 2+ ] i concentrations of 300 single cells (gray lines) measured in two independent experiments. The mean [Ca 2+ ] i signal averaged over all cells responding to XCL1 is indicated (black line).

Article Snippet: The lower chamber was filled with chemotaxis medium containing recombinant human XCL1 (R&D Systems) or any of the chemokines CCL2 (100 ng/ml), CCL21 (200 ng/ml), CX3CL1 (1 ng/ml), CXCL12 (200 ng/ml for T cells, B cells, NK cells, and monocytes; 100 ng/ml for pDCs), and CXCL8 (100 ng/ml; all from R&D Systems), and the cells were incubated for 150 min at 37°C in 5% CO 2 .

Techniques: Imaging, Positive Control

Journal: The Journal of Experimental Medicine

Article Title: Superior antigen cross-presentation and XCR1 expression define human CD11c + CD141 + cells as homologues of mouse CD8 + dendritic cells

doi: 10.1084/jem.20100348

Figure Lengend Snippet: XCL1 selectively induces chemotaxis in CD141 + DCs. (A) A mixture of highly purified, flow-sorted DC subtypes (20% CD141 + , 40% CD16 + , and 40% CD1c + DCs; Input DC) was tested for migration in response to medium alone or to serial dilutions of XCL1 (10–5,000 ng/ml) in a Transwell system. A combination of the chemokines CCL2, CCL21, and CX3CL1 was used as a positive control for the DC subsets (Migrated DC). The absolute numbers of CD141 + , CD1c + , and CD16 + DCs in input and migrated cell populations are truly represented in the dot plots, because all cells within a defined volume were included in the analysis in each instance. (B) Proportion of migrated CD1c + , CD16 + , and CD141 + DCs in the experiment shown in A. (C) Proportion of migrated pDCs, monocytes, granulocytes, T cells, B cells, and NK cells in response to XCL1 (10–1,000 ng/ml) or the chemokines CXCL12 and CXCL8, which were used as positive controls. For migration assays of B cells, NK cells, and monocytes, PBMCs were magnetically depleted of T cells, and for T cell migration, PBMCs were used directly. For migration assays of granulocytes, whole blood cells were used after erythrocyte lysis with ACK buffer, and pDCs were magnetically enriched from PBMCs with the Plasmacytoid Dendritic Cell Isolation Kit (Miltenyi Biotec). All experiments with DCs were performed three times; all other populations were assayed twice. Error bars represent means ± SEM.

Article Snippet: The lower chamber was filled with chemotaxis medium containing recombinant human XCL1 (R&D Systems) or any of the chemokines CCL2 (100 ng/ml), CCL21 (200 ng/ml), CX3CL1 (1 ng/ml), CXCL12 (200 ng/ml for T cells, B cells, NK cells, and monocytes; 100 ng/ml for pDCs), and CXCL8 (100 ng/ml; all from R&D Systems), and the cells were incubated for 150 min at 37°C in 5% CO 2 .

Techniques: Chemotaxis Assay, Purification, Migration, Positive Control, Lysis, Cell Isolation

Journal: The Journal of Experimental Medicine

Article Title: Superior antigen cross-presentation and XCR1 expression define human CD11c + CD141 + cells as homologues of mouse CD8 + dendritic cells

doi: 10.1084/jem.20100348

Figure Lengend Snippet: Involvement of the XCL1–XCR1 communication axis in the innate and adaptive cytotoxic responses to cross-presented microbial and tumor antigens. Secretion of the chemokine XCL1 by activated NK cells specifically attracts XCR1-expressing DCs capable of antigen cross-presentation. This ensures an effective communication between these cells in the innate phase of the immune response. In the adaptive phase, secretion of XCL1 by activated CD8 + T cells optimizes the communication with antigen cross-presenting DCs and facilitates the differentiation of CD8 + T cells to cytotoxic cells.

Article Snippet: The lower chamber was filled with chemotaxis medium containing recombinant human XCL1 (R&D Systems) or any of the chemokines CCL2 (100 ng/ml), CCL21 (200 ng/ml), CX3CL1 (1 ng/ml), CXCL12 (200 ng/ml for T cells, B cells, NK cells, and monocytes; 100 ng/ml for pDCs), and CXCL8 (100 ng/ml; all from R&D Systems), and the cells were incubated for 150 min at 37°C in 5% CO 2 .

Techniques: Expressing