Journal: Age and ageing

Article Title: Moderate physical activity associated with a higher naïve/memory T-cell ratio in healthy old individuals: potential role of IL15.

doi: 10.1093/ageing/afaa035

Figure Lengend Snippet: Figure 1. Impact of physical activity on T-cell subset distribution, thymic output and B-cell subset distribution. Percentage of (a) naïve CD4 T cells, (b) memory CD4 T cells in sedentary (n = 25) and physically active healthy older participants (n = 25). (c) Association between serum IL-15 levels and peripheral frequency of naïve CD4 T cells (n = 50). Percentage of (d) PTK7+ve

Article Snippet: For phenotypic characterisation of T-cell subset distribution, PBMCs (1 × 106/ml) were stained with a combination of fluorochrome-conjugated antibodies, anti-human CD3-PEcy7 (eBiosciences, clone UCHT1), anti-human CD4 Violet (eBiosciences, clone RPA-T4), anti-human CD8 PE (Immunotools, clone UCHT4), antihuman CCR7 FITC (R and D systems, clone 150,503), anti-human CD45RA APC (Biolegend, clone HI-100) and anti-human PTK7 (Miltenyi Biotech, clone 188B).

Techniques: Activity Assay

Journal: Scientific Reports

Article Title: Tuning IL-2 signaling by ADP-ribosylation of CD25

doi: 10.1038/srep08959

Figure Lengend Snippet: Splenocytes from DEREG mice were incubated in the absence or presence of the indicated concentrations of NAD + before staining with fluorochrome-conjugated mAbs directed against CD4 and CD25 (mAb PC61 or mAb 7D4). Gating was performed on CD4 + cells. (a) Representative dot plots of cells incubated in the absence or presence of 12 μM NAD + . (b) Percentages of CD4 + GFP + cells staining with anti-CD25 mAbs plotted as a function of the concentration of added NAD + . Results are representative of two independent experiments.

Article Snippet: Alternatively, after erythrocyte lysis with Ack lysis buffer (Bio Whittaker) and depletion of B-cells with Dynabead-conjugated sheep anti-mouse IgG (Invitrogen), Tregs were positively selected by magnetic cell sorting using PE conjugated anti-CD25 (7D4) and magnetic bead-conjugated anti-PE antibodies according to the manufacturer's instructions (Miltenyi Biotec).

Techniques: Incubation, Staining, Concentration Assay