Journal: Methods in enzymology

Article Title: Development and validation of a method to deliver vitamin A to macrophages

doi: 10.1016/bs.mie.2022.04.008

Figure Lengend Snippet: 2.1. Equipment

Article Snippet: Glass plates for electrophoresis Bio-Rad 1655308 1653312 29.

Techniques: Sterility, High Performance Liquid Chromatography, Software, Spectrophotometry, Real-time Polymerase Chain Reaction, Membrane, Electrophoresis

Journal: bioRxiv

Article Title: The Enterococcal Polysaccharide Antigen: from structure to biosynthesis and function

doi: 10.1101/2024.06.26.600781

Figure Lengend Snippet: a , Schematic of EPA treatment with hydrofluoric (HF) acid to produce OS1-OS3. b , Anomeric regions of the H- C HSQC spectra acquired on OS1-OS3. Structure of fragments c , OS1 and d , OS2. e , OS3 P NMR spectrum. f , Assignment of phosphodiester linkage units in OS3. g , OS3 structure.

Article Snippet: NMR spectra were recorded on a 900 MHz Bruker Advance NEO using a 5-mm-diameter triple-resonance inverse cryoprobe ( H and C; EPA fragments OS1 and OS2), a 600 MHz Bruker AVANCE III using a 5 mm PABBO BB-1H/D Z-GRD Probe ( H, C, and P; OS3), a 600 MHz NEO using a 5-mm-diameter TCI cryo-probe ( H and C; EPA, EPA_epaR, EPA_11720, EPA_11715, EPA_11714 and EPA_11706) and a 500 MHz Bruker Advance II using a 5-mm-diameter BBO probe ( H and P; OS1, OS2, EPA, EPA_epaR, EPA_11720, EPA_11715, EPA_11714 and EPA_11706).

Techniques:

Journal: Cancer Biology & Therapy

Article Title: Sulfatase 2 inhibition sensitizes triple-negative breast cancer cells to paclitaxel through augmentation of extracellular ATP

doi: 10.1080/15384047.2025.2483989

Figure Lengend Snippet: ELISA analysis of sulfatases and SULF2 immunohistochemistry. ELISAs were performed to examine the basal levels of (a) SULF1 and (b) SULF2 in adjusted cell supernatants inversely proportionate to the protein concentration in the corresponding cell lysate of MCF-10A cell line, TNBC MDA-MB 231, Hs 578t and MDA-MB 468 cell lines and ER+/PR+ T47D, MCF-7 and ZR-75-1 cell lines. MCF-10A expressed more SULF1 in comparison to the TNBCs but similar expression levels were observed in ER+/PR+ T47D and MCF-7 cells. However, the ER+/PR+ ZR-75-1 cell line expressed the most SULF1. Meanwhile, TNBCs and ER+/PR+ T47D and ZR-75-1 cells expressed more SULF2 in comparison to MCF-10A cells. Standard deviation was calculated from three independent experiments performed in triplicate. The student’s t-test was performed to determine significance with *representing p < 0.05 and **representing p < 0.01 comparing the protein expression MCF-10A to the protein expressions of TNBC cell lines. (b) The AMSBIO BR1202B breast cancer tissue array (120 cores with 82 TNBC cores, 20 ER+/PR+ cores, 14 HER2+ cores, and 4 necrotic cores; key can be found in supplemental Figure S6C) was stained with SULF2.

Article Snippet: The student’s t-test was performed to determine significance with *representing p < 0.05 and **representing p < 0.01 comparing the protein expression MCF-10A to the protein expressions of TNBC cell lines. (b) The AMSBIO BR1202B breast cancer tissue array (120 cores with 82 TNBC cores, 20 ER+/PR+ cores, 14 HER2+ cores, and 4 necrotic cores; key can be found in supplemental Figure S6C) was stained with SULF2.

Techniques: Enzyme-linked Immunosorbent Assay, Immunohistochemistry, Protein Concentration, Comparison, Expressing, Standard Deviation, Staining

Journal: Cancer Biology & Therapy

Article Title: Sulfatase 2 inhibition sensitizes triple-negative breast cancer cells to paclitaxel through augmentation of extracellular ATP

doi: 10.1080/15384047.2025.2483989

Figure Lengend Snippet: Statistical analysis for SULF2 immunohistochemistry. For the breast cancer tissue array and slides stained for heparanase: TNBC ( n =75), ER+/PR+ ( n =18), HER2+ ( n =14), (a) images were taken of SULF2-stained AMSBIO BR1202B breast cancer tissue array on an evos FL auto 2 microscope (40×). (b) Pairwise comparisons using Dunn’s test indicated that there was a significant difference in H-score between breast cancer sub-types TNBC and ER+/PR+ ( p = 0.0392). There was no significant different between TNBC and HER2+. (c) The Kruskal-Wallis test indicated that there was no significant difference in the average percentages of cells that stained positively for SULF2 in tissue sections of TNBC, ER+/PR+ breast cancer, and HER2+ breast cancer. (d) Pairwise comparisons using Dunn’s test indicated that there was a significant difference between breast cancer sub-types TNBC and ER+/PR+ ( p = 0.009), and TNBC and HER2+ ( p = 0.0338), in the percentages of cells that stained moderately or strongly positive for SULF2. (e) The same Dunn’s test from (d) also indicated that there was a significant difference between cancer sub-types TNBC and ER+/PR+ ( p = 0.0094), and TNBC and HER2+( p = 0.0338), in the percentages of cells that stained negative or weakly positive for SULF2. (f) Pairwise comparisons using Dunn’s test showed that there was a significant difference between TNBC breast cancer stages 2A and 2B ( p = 0.0007) in the percentages of cells that stained positively for SULF2. No other differences among different TNBC stages were significant. Standard error of the mean was calculated. For statistical analysis, *representing p < 0.05, **representing p < 0.01 and ***representing p < 0.001.

Article Snippet: The student’s t-test was performed to determine significance with *representing p < 0.05 and **representing p < 0.01 comparing the protein expression MCF-10A to the protein expressions of TNBC cell lines. (b) The AMSBIO BR1202B breast cancer tissue array (120 cores with 82 TNBC cores, 20 ER+/PR+ cores, 14 HER2+ cores, and 4 necrotic cores; key can be found in supplemental Figure S6C) was stained with SULF2.

Techniques: Immunohistochemistry, Staining, Microscopy