Journal: Radiation research

Article Title: Neurological Impairments in Mice Subjected to Irradiation and Chemotherapy

doi: 10.1667/RR15540.1

Figure Lengend Snippet: Combined treatment alters hippocampal serotonin receptor 5HT1A receptor (5HT1AR) levels. Panels A and B: Representative images of 5HT1AR immunohistochemistry in the CA1 region of the hippocampus in control and combined treatment mice at 5 weeks postirradiation, respectively. Panel C: Combined treatment mice had reduced numbers of 5HT1AR puncta per μm3 × 100 in the CA1 region of the hippocampus compared to control mice at 5 weeks postirradiation (0.92 ± 0.1 and 2.7 ± 0.1, respectively). Panels D and E: Representative images of 5HT1A receptor immunohistochemistry in the CA3 region of the hippocampus in control and combined treatment mice at 5 weeks postirradiation, respectively. Panel F: At 5 weeks postirradiation, fewer 5HT1AR puncta were also observed in the CA3 region of the hippocampus of combined treatment compared to control mice (0.92 ± 0.1 and 2.02 ± 0.2, respectively). Panels G and H: Representative images of 5HT1A receptor immunohistochemistry in the CA1 region of the hippocampus in control and combined treatment mice at 15 weeks postirradiation, respectively, at which time (panel I), combined treatment mice had reduced numbers of 5HT1AR puncta in the CA1 region of the hippocampus compared to control mice (1.5 ± 0.1 and 3.3 ± 0.4, respectively). Panels J and K: Representative images of 5HT1A receptor immunohistochemistry in the CA3 region of the hippocampus in control and combined treatment mice, respectively, at 15 weeks postirradiation, at which time (panel L) levels of 5HT1AR in the CA3 region of the hippocampus of combined treatment mice were indistinguishable from that of controls (2.5 ± 0.4 and 3.2 ± 0.6, respectively). Red indicates 5HT1AR; blue indicates DAPI counterstain. Scale bar = 20 μm. SP = stratum pyramidale, SR = stratum radiatum and SLM = stratum lacunosum moleculare. Data are presented as mean ± SEM for n = 4 mice/group. P values are derived using Mann-Whitney U test. **P < 0.01, ***P < 0.001.

Article Snippet: Sections were initially washed in PBS (pH 7.4), blocked for 30 min in PBS containing 2% (wt/vol) bovine serum albumin (BSA), then incubated for 24 h in a primary antibody mixture of PBS containing 1% BSA, 0.1% Triton X-100 and rabbit polyclonal 5HT1AR (1:100; reacts with mouse and affinity purified by protein A; Biorbyt LLC, St. Louis, MO).

Techniques: Immunohistochemistry, Derivative Assay, MANN-WHITNEY

Journal: International Journal of Molecular Sciences

Article Title: Distinct Effects of Escitalopram and Vortioxetine on Astroglial L -Glutamate Release Associated with Connexin43

doi: 10.3390/ijms221810013

Figure Lengend Snippet: Summary of the effects of acute and subchronic administrations of escitalopram (ESC), vortioxetine (VTX), BP554 (BP), WAY100635 (WAY) and SB269970 (SB) on astroglial L-glutamate release and protein expression of Cx43, 5-HT1AR, 5-HT7R and phosphorylated ERK in the astrocyte.

Article Snippet: The antibodies of GAPDH (NB300-322, 1:100, Novus Biologicals, Littleton, CO, USA), Cx43 (C6219, 1:100, Sigma-Aldrich, St. Louis, MO, USA), Erk (AF1576, 10 μg/mL, R&D systems, Minneapolis, MN, USA), pErk (AF1018, 5 μg/mL, R&D systems), 5-HT7R (NB100-56352, 1:50, Novus Biologicals) and 5-HT1AR (NBP2-21590, 1:100, Novus Biologicals) were diluted in an antibody diluent (Immuno shot plutinum) [ ].

Techniques: Expressing, Clinical Proteomics, Membrane

Journal: Frontiers in Psychology

Article Title: Effects of Acupuncture on Chronic Stress-Induced Depression-Like Behavior and Its Central Neural Mechanism

doi: 10.3389/fpsyg.2019.01353

Figure Lengend Snippet: Acupuncture treatment at AP (KI10·LR8·LU8·LR4) changes 5-HT1A receptor expression in the cortex, hippocampus, thalamus, and hypothalamus. (A,B) Acupuncture treatment at AP (KI10·LR8·LU8·LR4) significantly increased 5-HT1A expression in the cortex, hippocampus, thalamus, and hypothalamus as compared to the CRS group. NOR, normal; AP, CRS and acupuncture treatment at KI10·LR8·LU8·LR4; NP, CRS and acupuncture treatment at non-acupoints on the hips. * p < 0.05, ** p < 0.01, *** p < 0.001 vs. NOR. # p < 0.05, ## p < 0.01, ### p < 0.001 vs. CRS. One-way ANOVA followed by the Newman-Keuls test. Error bars indicate SEM.

Article Snippet: The details of the primary antibodies were as follows: c-Fos monoclonal antibody (1:150, Santa Cruz, Dallas, TX, USA), 5-HT1A (1:100, Novus Biologicals, Centennial, CO, USA), and 5-HT1B (1:100, Abcam, Cambridge, UK).

Techniques: Expressing

Journal: Frontiers in Pharmacology

Article Title: Asiaticoside improves depressive-like behavior in mice with chronic unpredictable mild stress through modulation of the gut microbiota

doi: 10.3389/fphar.2024.1461873

Figure Lengend Snippet: Effects of asiaticoside on sucrose preference (A) , FST immobility time (B) , and OFT immobility time (C) in CUMS mice. Effects of asiaticoside on the expression of 5-HT1A (D) and BDNF (E) proteins in the hippocampus of CUMS mice (n = 6). Data are expressed as the mean with SD. # p < 0.05, ## p < 0.01, ### < 0.001 vs. CON group; * p < 0.05, ** p < 0.01, *** p < 0.001 vs. CUMS group.

Article Snippet: BDNF Polyclonal antibody was purchased from Proteintech Group, Inc. (Catalog No. 28205-1-AP), whereas the Anti-5HT1A Receptor antibody was purchased from Abcam (Catalog No. ab85615).

Techniques: Expressing