Journal: Frontiers in Neuroanatomy

Article Title: The identification and neurochemical characterization of central neurons that target parasympathetic preganglionic neurons involved in the regulation of choroidal blood flow in the rat eye using pseudorabies virus, immunolabeling and conventional pathway tracing methods

doi: 10.3389/fnana.2015.00065

Figure Lengend Snippet: Choroidal SSN is enriched in serotonergic terminals (A), and PRV+ SSN choroidal neurons (red) were seen to be contacted by serotonergic terminals (green) (B) . Moreover, choroidal SSN is enriched in 5HT2A serotonin receptors (C) .

Article Snippet: Antibodies against the following substances were used: nNOS (Santa Cruz SC-648, raised in rabbit, used at 1:200–1:800), calbindin (Sigma C9848, raised in mouse, used at 1:5000), or tyrosine hydroxylase (Immunostar, raised in mouse, used at 1:2000), the type-2 vesicular glutamate transporter (VGLUT2, Sigma V2514, raised in rabbit, used at 1:1000), serotonin (Immunostar, raised in rabbit, used at 1:10,000), the 5HT2A serotonin receptor (Immunostar, raised in rabbit, used at 1:1000), oxytocin (provided by Harold Gainer, NIH, raised in mouse, used at 1:500), and vasopressin (provided by Alan Robinson, retired, raised in rabbit, used at 1:20,000).

Techniques:

Journal:

Article Title: Glial Restricted Precursor Cell Transplant with Cyclic Adenosine Monophosphate Improved Some Autonomic Functions but Resulted in a Reduced Graft Size after Spinal Cord Contusion Injury in Rats

doi: 10.1016/j.expneurol.2010.10.011

Figure Lengend Snippet: Antibodies used for immunohistochemistry. Source, concentration, and incubation time are shown. The Nestin Rat-401 hybridoma was developed by Susan Hockfield and obtained from the Developmental Systems Hybridoma Bank (DSHB) developed under the auspices of the NICHD and maintained by the University of Iowa, Department of Biological Sciences, Iowa City, IA, USA. The PDGFR〈 was generously supplied by Dr. Stallcup, Burnham Institute for Medical Research, La Jolla, CA.

Article Snippet: Transverse sections were also stained with Luxol Fast Blue for myelin to determine white matter sparing. table ft1 table-wrap mode="anchored" t5 caption a7 Primary antibody Secondary antibody Buffer Name [ ] Inc. time Isotype Source Name [ ] Inc. time Source hPLAP 1:200 O/N Mouse monoclonal IgG2a Sigma, St. Louis, MO AF-594 GAM IgG2a 1:200 2 hours Invitrogen, Carlsbad, CA PBS AF-488 GAM IgG 1:200 2 hours Invitrogen PBS CC-1 1:100 O/N Mouse monoclonal IgG2b Abcam Cambridge, MA AF-488 GAM IgG2b 1:200 2 hours Invitrogen PBS GFAP 1:1000 O/N Rabbit polyclonal IgG Sigma AF-488 GAR IgG 1:200 2 hours Invitrogen PBS Nestin 1:10 O/N Mouse hybridoma supernatant IgG1 DSHB, Iowa City, IA US AF-488 GAM IgG1 1:200 2 hours Invitrogen PBS NG2 1:200 O/N Mouse monoclonal IgG1 Biological, Swampscott, MA AF-488 GAM IgG1 1:200 2 hours Invitrogen PBS PDGFRα 1:50 O/N Rabbit polyclonal IgG Dr. Stallcup, La Jolla, CA AF-488 GAR IgG 1:200 2 hours Invitrogen PBS 5-Ht 1:500 O/N Rabbit polyclonal IgG Immunostar, Hudson, WI AF-488 GAR IgG 1:200 2 hours Invitrogen PBS Syn.

Techniques: Immunohistochemistry, Concentration Assay, Incubation