Journal: British Journal of Cancer

Article Title: YES1 amplification confers trastuzumab–emtansine (T-DM1) resistance in HER2-positive cancer

doi: 10.1038/s41416-020-0952-1

Figure Lengend Snippet: a Signalling proteins in indicated cells were determined by western blotting. BT-474, BT-474/YES1 WT and BT-474/YES1 Y537F cells ( b ), and SK-OV-3, SK-OV-3/YES1 WT and SK-OV-3/YES1 Y537F cells ( c ), were treated with different concentrations of T-DM1, trastuzumab or lapatinib for 120 h, after which cell survival was measured using sulforhodamine B assays. BT-474/YES1 Y537F ( d ) and SK-OV-3/YES1 Y537F ( e ) cells were treated with different concentrations of the HER2-targeted inhibitors, T-DM1 or lapatinib, for 120 h, with or without dasatinib (30 nM), after which cell survival was measured using sulforhodamine B assays. Data shown represent means ± SD of three independent experiments.

Article Snippet: BT-474 and SK-OV-3 cells expressing YES1 Y537F and YES1 WT were generated by transfecting cells with the YES1 Y537F (Addgene plasmid #51299) and YES1 WT (generated by point mutation from YES1 Y537F plasmid) plasmids, respectively.

Techniques: Western Blot

Journal: British Journal of Cancer

Article Title: YES1 amplification confers trastuzumab–emtansine (T-DM1) resistance in HER2-positive cancer

doi: 10.1038/s41416-020-0952-1

Figure Lengend Snippet: Mice bearing BT-474/R1-7 ( a ) or SK-OV-3/YES1 Y537F ( b ) xenograft tumours were treated with T-DM1, dasatinib, or a combination of T-DM1 and dasatinib. Tumour volumes (top) and body weights (bottom) were measured on the indicated days. Data shown represent means ± SD (error bars; control group, n = 10 or treatment groups, n = 6; * p < 0.05, ** p < 0.01, **** p < 0.0001). c BT-474/R1-7 xenograft tumours were isolated, then signalling proteins were determined by western blotting. d Proposed model of T-DM1 resistance in BT-474/R1-7 cells.

Article Snippet: BT-474 and SK-OV-3 cells expressing YES1 Y537F and YES1 WT were generated by transfecting cells with the YES1 Y537F (Addgene plasmid #51299) and YES1 WT (generated by point mutation from YES1 Y537F plasmid) plasmids, respectively.

Techniques: Control, Isolation, Western Blot

Journal: The ISME Journal

Article Title: The soil microbial food web revisited: Predatory myxobacteria as keystone taxa?

doi: 10.1038/s41396-021-00958-2

Figure Lengend Snippet: Myxobacteria and potential prey organisms used in predation assays.

Article Snippet: The myxobacteria Haliangium ochraceum , Stigmatella aurantiaca , Kofleria flava , Corallococcus coralloides, and Chondromyces robustus were purchased from the German Collection of Microorganisms and Cell Cultures (DSMZ, Table ).

Techniques: Bacteria

Journal: Biomedicines

Article Title: The Antibacterial Activity of Human Amniotic Membrane against Multidrug-Resistant Bacteria Associated with Urinary Tract Infections: New Insights from Normal and Cancerous Urothelial Models

doi: 10.3390/biomedicines9020218

Figure Lengend Snippet: List of bacterial strains used in the experiments.

Article Snippet: Enterococcus faecalis , Reference strain; vancomycin-resistant, vanB -positive strain , Gram-positive , ATCC 51299.

Techniques: Staining