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normal human tracheal epithelial cells nhte  (Cell Applications Inc)
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Cell Applications Inc normal human tracheal epithelial cells nhte
Figure 2. Evaluation of SARS-CoV-2 variants in primary <t>epithelial</t> cells and K18-hACE2 mouse model. (A) Normal human primary tracheal epithelial cells <t>(NHTE)</t> and normal human primary nasal epithelial cells (NHNE) were inoculated with a MOI = 1 and virus titer were assessed at 1 to 3 dpi with a plaque assay. Data are represented as mean ± standard error of the mean. Virus isolates within the A lineage, WA1/2020 (red), and A.1, UT21 (green), are represented by dashed lines. Virus isolates within the B.1 lineage, UT12 (black), UT23 (navy blue), UT27 (orange), and UT29 (violet), and B.1.577, UT5 (brown), are represented by solid lines. In B-D, female K18-hACE2 mice were intranasally challenged at 104 PFU with either SARS-CoV-2 WA1/2020 (n = 2) or one of six clinical isolates UT5, UT12, UT21, UT23, UT27, or UT29 (n = 3). Graphs of lung viral titers (B), weight and body temperature (C), or selected immune responses in lung (D) are presented.
Normal Human Tracheal Epithelial Cells Nhte, supplied by Cell Applications Inc, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Figure 2. Evaluation of SARS-CoV-2 variants in primary epithelial cells and K18-hACE2 mouse model. (A) Normal human primary tracheal epithelial cells (NHTE) and normal human primary nasal epithelial cells (NHNE) were inoculated with a MOI = 1 and virus titer were assessed at 1 to 3 dpi with a plaque assay. Data are represented as mean ± standard error of the mean. Virus isolates within the A lineage, WA1/2020 (red), and A.1, UT21 (green), are represented by dashed lines. Virus isolates within the B.1 lineage, UT12 (black), UT23 (navy blue), UT27 (orange), and UT29 (violet), and B.1.577, UT5 (brown), are represented by solid lines. In B-D, female K18-hACE2 mice were intranasally challenged at 104 PFU with either SARS-CoV-2 WA1/2020 (n = 2) or one of six clinical isolates UT5, UT12, UT21, UT23, UT27, or UT29 (n = 3). Graphs of lung viral titers (B), weight and body temperature (C), or selected immune responses in lung (D) are presented.

Journal: Viruses

Article Title: Dissecting Phenotype from Genotype with Clinical Isolates of SARS-CoV-2 First Wave Variants.

doi: 10.3390/v15030611

Figure Lengend Snippet: Figure 2. Evaluation of SARS-CoV-2 variants in primary epithelial cells and K18-hACE2 mouse model. (A) Normal human primary tracheal epithelial cells (NHTE) and normal human primary nasal epithelial cells (NHNE) were inoculated with a MOI = 1 and virus titer were assessed at 1 to 3 dpi with a plaque assay. Data are represented as mean ± standard error of the mean. Virus isolates within the A lineage, WA1/2020 (red), and A.1, UT21 (green), are represented by dashed lines. Virus isolates within the B.1 lineage, UT12 (black), UT23 (navy blue), UT27 (orange), and UT29 (violet), and B.1.577, UT5 (brown), are represented by solid lines. In B-D, female K18-hACE2 mice were intranasally challenged at 104 PFU with either SARS-CoV-2 WA1/2020 (n = 2) or one of six clinical isolates UT5, UT12, UT21, UT23, UT27, or UT29 (n = 3). Graphs of lung viral titers (B), weight and body temperature (C), or selected immune responses in lung (D) are presented.

Article Snippet: Normal human tracheal epithelial cells (NHTE) donated from a 42-year-old Caucasian male were purchased through Cell Applications Cat# 504-05a, Lot# 2559.

Techniques: Virus, Plaque Assay