Journal: Endocrinology

Article Title: Identification and cloning of a novel androgen-responsive gene, uridine diphosphoglucose dehydrogenase, in human breast cancer cells.

doi: 10.1210/endo.140.10.7071

Figure Lengend Snippet: FIG. 1. Differential display PCR analysis. A, Human ZR-75–1 breast cancer cells were grown for 48 h in the presence of estradiol (10210 M) alone (control) or in combination with DHT (1029 M). Total RNA was isolated from control and DHT-treated cells and used for cDNA syn- thesis in the presence of oligo(dT) primer and Moloney murine leu- kemia virus reverse transcriptase. A fraction of each cDNA was am- plified by PCR using P4 and T1 primers, [a-33P]dATP, and dNTP mix. The products were analyzed on a denaturing 6% acrylamide gel. The gel was dried and exposed to x-ray film. B, The differentially displayed cDNA was amplified by PCR using the P4 and T1 primers and was separated on a 1% agarose gel along with DNA size markers.

Article Snippet: 17b-Estradiol (E2) and DHT were purchased from Steraloids (Wilton, NH).

Techniques: Control, Isolation, Virus, Reverse Transcription, Acrylamide Gel Assay, Amplification, Agarose Gel Electrophoresis

Journal: Endocrinology

Article Title: Identification and cloning of a novel androgen-responsive gene, uridine diphosphoglucose dehydrogenase, in human breast cancer cells.

doi: 10.1210/endo.140.10.7071

Figure Lengend Snippet: FIG. 2. Northern blot analysis of androgen-regulated mRNAs. Total RNA was isolated from ZR-75–1 cells grown for 48 h in the presence of estradiol (10210 M) alone (control) or in combination with DHT (1029

Article Snippet: 17b-Estradiol (E2) and DHT were purchased from Steraloids (Wilton, NH).

Techniques: Northern Blot, Isolation, Control

Journal: Endocrinology

Article Title: Identification and cloning of a novel androgen-responsive gene, uridine diphosphoglucose dehydrogenase, in human breast cancer cells.

doi: 10.1210/endo.140.10.7071

Figure Lengend Snippet: FIG. 6. Time course of DHT treatment on UDPGDH mRNA levels in ZR-75–1 cells. ZR-75–1 cells were grown for 0, 1, 6, 24, and 48 h in medium containing estradiol (10210 M) supplemented with DHT (1029

Article Snippet: 17b-Estradiol (E2) and DHT were purchased from Steraloids (Wilton, NH).

Techniques:

Journal: Endocrinology

Article Title: Identification and cloning of a novel androgen-responsive gene, uridine diphosphoglucose dehydrogenase, in human breast cancer cells.

doi: 10.1210/endo.140.10.7071

Figure Lengend Snippet: FIG. 7. Northern analysis of UDPGDH mRNA levels after DHT, cy- cloheximide, actinomycin D, and hydroxyflutamide treatment in ZR- 75–1 cells. ZR-75–1 cells were grown in medium containing estradiol (10210 M) supplemented with 1029 M DHT, DHT plus cycloheximide, DHT plus actinomycin D, and DHT plus hydroxyflutamide (3 3 1026

Article Snippet: 17b-Estradiol (E2) and DHT were purchased from Steraloids (Wilton, NH).

Techniques: Northern Blot

Journal: Toxics

Article Title: Determination of 19 Steroid Hormones in Human Serum and Urine Using Liquid Chromatography-Tandem Mass Spectrometry

doi: 10.3390/toxics10110687

Figure Lengend Snippet: Target analytes (19 steroid hormones), isotopically labeled internal standards, and their MRM parameters. MRM parameters include precursor ion (Q1), product ion (Q3), declustering potential (DP), entrance potential (EP), collision energy (CE), collision cell exit potential (CXP), and dwell time.

Article Snippet: Individual certified stock solutions of cortisone, 11-deoxycortisol, aldosterone, corticosterone, progesterone, 17α-OH-progesterone, pregnenolone, estrone, estradiol, testosterone, dehydroepiandrosterone (DEHA), 5α-dihydrotestosterone, 17α-OH-pregnenolone, 11-dehydrocorticosterone, androstenediol, 13 C 3 -cortisone, 13 C 3 -cortisol, 13 C 3 -11-deoxycortisol, D 4 -aldosterone, D 4 -corticosterone, 13 C 3 -progesterone, 13 C 3 -17α-OH-progesterone, 13 C 2 -D 2 -pregnenolone, 13 C 3 -estrone, 13 C 2 -estradiol, 13 C 3 -estriol, 13 C 3 -testosterone, 13 C 3 -DEHA, 13 C 3 -5α-OH-dihydrotestosterone, and 13 C 2 -D 2 -17α-OH-pregnenolone (100 μg/mL) with purities of 95–98% were purchased from Cambridge Isotope Laboratories (Andover, MA, USA).

Techniques: Labeling

Journal: Toxics

Article Title: Determination of 19 Steroid Hormones in Human Serum and Urine Using Liquid Chromatography-Tandem Mass Spectrometry

doi: 10.3390/toxics10110687

Figure Lengend Snippet: Method validation parameters for the analysis of 19 steroid hormones in human urine.

Article Snippet: Individual certified stock solutions of cortisone, 11-deoxycortisol, aldosterone, corticosterone, progesterone, 17α-OH-progesterone, pregnenolone, estrone, estradiol, testosterone, dehydroepiandrosterone (DEHA), 5α-dihydrotestosterone, 17α-OH-pregnenolone, 11-dehydrocorticosterone, androstenediol, 13 C 3 -cortisone, 13 C 3 -cortisol, 13 C 3 -11-deoxycortisol, D 4 -aldosterone, D 4 -corticosterone, 13 C 3 -progesterone, 13 C 3 -17α-OH-progesterone, 13 C 2 -D 2 -pregnenolone, 13 C 3 -estrone, 13 C 2 -estradiol, 13 C 3 -estriol, 13 C 3 -testosterone, 13 C 3 -DEHA, 13 C 3 -5α-OH-dihydrotestosterone, and 13 C 2 -D 2 -17α-OH-pregnenolone (100 μg/mL) with purities of 95–98% were purchased from Cambridge Isotope Laboratories (Andover, MA, USA).

Techniques: Biomarker Discovery

Journal: Toxics

Article Title: Determination of 19 Steroid Hormones in Human Serum and Urine Using Liquid Chromatography-Tandem Mass Spectrometry

doi: 10.3390/toxics10110687

Figure Lengend Snippet: Method validation parameters for the analysis of 19 steroid hormones in human serum.

Article Snippet: Individual certified stock solutions of cortisone, 11-deoxycortisol, aldosterone, corticosterone, progesterone, 17α-OH-progesterone, pregnenolone, estrone, estradiol, testosterone, dehydroepiandrosterone (DEHA), 5α-dihydrotestosterone, 17α-OH-pregnenolone, 11-dehydrocorticosterone, androstenediol, 13 C 3 -cortisone, 13 C 3 -cortisol, 13 C 3 -11-deoxycortisol, D 4 -aldosterone, D 4 -corticosterone, 13 C 3 -progesterone, 13 C 3 -17α-OH-progesterone, 13 C 2 -D 2 -pregnenolone, 13 C 3 -estrone, 13 C 2 -estradiol, 13 C 3 -estriol, 13 C 3 -testosterone, 13 C 3 -DEHA, 13 C 3 -5α-OH-dihydrotestosterone, and 13 C 2 -D 2 -17α-OH-pregnenolone (100 μg/mL) with purities of 95–98% were purchased from Cambridge Isotope Laboratories (Andover, MA, USA).

Techniques: Biomarker Discovery

Journal: Toxics

Article Title: Determination of 19 Steroid Hormones in Human Serum and Urine Using Liquid Chromatography-Tandem Mass Spectrometry

doi: 10.3390/toxics10110687

Figure Lengend Snippet: Steroid hormones measured in plasma Standard Reference Material (SRM1950) using the method developed in this study and compared to the certified reference values. Reference values were only available for cortisol, progesterone, and testosterone.

Article Snippet: Individual certified stock solutions of cortisone, 11-deoxycortisol, aldosterone, corticosterone, progesterone, 17α-OH-progesterone, pregnenolone, estrone, estradiol, testosterone, dehydroepiandrosterone (DEHA), 5α-dihydrotestosterone, 17α-OH-pregnenolone, 11-dehydrocorticosterone, androstenediol, 13 C 3 -cortisone, 13 C 3 -cortisol, 13 C 3 -11-deoxycortisol, D 4 -aldosterone, D 4 -corticosterone, 13 C 3 -progesterone, 13 C 3 -17α-OH-progesterone, 13 C 2 -D 2 -pregnenolone, 13 C 3 -estrone, 13 C 2 -estradiol, 13 C 3 -estriol, 13 C 3 -testosterone, 13 C 3 -DEHA, 13 C 3 -5α-OH-dihydrotestosterone, and 13 C 2 -D 2 -17α-OH-pregnenolone (100 μg/mL) with purities of 95–98% were purchased from Cambridge Isotope Laboratories (Andover, MA, USA).

Techniques: Clinical Proteomics