Journal: BMC Pharmacology & Toxicology

Article Title: A novel nitidine chloride nanoparticle overcomes the stemness of CD133 + EPCAM + Huh7 hepatocellular carcinoma cells for liver cancer therapy

doi: 10.1186/s40360-022-00589-z

Figure Lengend Snippet: a . Representative organ images showing specific tumor targeting of rhodamine B isothiocyanate labeled TPGS-FA/NC nanoparticles 8 h post-injection into mice bearing Huh7 xenograft (T: tumor, Li: liver, H: heart, L: lung, K:kidney, S: spleen, and B:brain; Color scale: radiant efficiency, [p s − 1 cm − 2 sr − 1 ] [μWcm − 2 ] − 1 ). V b . Quantitative analysis of biodistribution in tumors and normal organs, quantified from the organ images. Intravenous treatment of nude mice bearing orthotopic Huh7 xenografts with TPGS-FA/NC nanoparticles (red) and control groups (turquoise: NC, fuchsia:5-Fu, blue: PBS) every other day for a total of five injections (4 mg kg − 1,NC per body weight, indicated by arrows). c . Mice body weight was monitored during the time course of treatments ( n = 5 biologically independent animals, statistics was calculated by two-tailed unpaired t-test presented as mean ± SD, * p < 0.05, ** p < 0.01, *** p < 0.001, p = 4.3 × 10 − 3 ,3.4 × 10 − 3 and 5.0 × 10 − 4 comparing TPGS-FA/NC to NC,5-Fu and PBS, respectively). d . Representative images of liver cancer tumors harvested from mice after treatments * p < 0.05, ** p < 0.01, *** p < 0.001; p = 0.01, 8 × 10 − 4,and 2 × 10 − 4 comparing TPGS-FA/NC to NC, 5-Fu, and PBS, respectively. Source data are provided as a

Article Snippet: 5-fluorouracil (5-Fu) was purchased from MedChemExpress (MCE, Monmouth Junction, NJ, USA).

Techniques: Labeling, Injection, Control, Two Tailed Test

Journal: Food Science and Biotechnology

Article Title: Pine needle hexane extract promote cell cycle arrest and premature senescence via p27 KIP1 upregulation gastric cancer cells

doi: 10.1007/s10068-019-00730-5

Figure Lengend Snippet: The effect of PNH and anti-cancer agent combination treatment on gastric cancer cells. A, B Combined treatment with PNH alone (40 μg/ml) and anticancer agents 5-FU alone (2 μM in AGS, 6 μM in SNU-668), PTX alone (2 nM in AGS, 10 nM in SNU-668 or a combination of half concentration, enhanced the therapeutic effects on AGS(A) and SNU-668(B) cells. The error bars indicate 95% confidence intervals; *P < 0.0001 using the two-sided t-test and **P < 0.005. C, D Cell cycle populations were examined by PI staining after 5-FU, PTX and PNH alone or combination treatment for 48 h in AGS(C) and SNU-668(D) cells

Article Snippet: Inhibition of cell proliferation by PNH alone or in combination with 5-FU (TargetMol, Wellesley Hills, MA, USA) or Paclitaxel (PTX) (TargetMol) was measured using the WST-1 assay same method.

Techniques: Concentration Assay, Staining