Journal: bioRxiv

Article Title: A Novel Hollow Fiber Infection Model (HFIM) for Antiviral PK/PD studies of CMV infection

doi: 10.64898/2026.03.13.710048

Figure Lengend Snippet: Circle and square lines represent the Towne (lab-adapted) and the Merlin (wild-type) strain, respectively. ( A, B ) represent the genome copies (copies/mL) and virus titer (PFU/mL), over time. ( C, D ) represent the log 10 fold change relative to quantified copies/mL and PFU/mL at day of inoculation.

Article Snippet: Human cytomegalovirus (HCMV) Towne (ATCC ® VR-977 TM) and the Merlin strains were used for the infection model.

Techniques: Virus

Journal: bioRxiv

Article Title: A Novel Hollow Fiber Infection Model (HFIM) for Antiviral PK/PD studies of CMV infection

doi: 10.64898/2026.03.13.710048

Figure Lengend Snippet: Ganciclovir treatment was administered 2 mg/hour every 12 hours, 3 days post-inoculation. Circle and square lines represent the control (no drug treatment) and drug treatment group. (A-D) represent the log10-fold change of genome copies (copies/mL) and virus titers (PFU/mL) for Towne and Merlin, respectively, over 72 hours post-treatment initiation.

Article Snippet: Human cytomegalovirus (HCMV) Towne (ATCC ® VR-977 TM) and the Merlin strains were used for the infection model.

Techniques: Control, Virus

Journal: npj Viruses

Article Title: SARS-CoV-2 crossreactive B-cells outnumber seasonal coronavirus spike-specific clones at the end of the COVID-19 pandemic

doi: 10.1038/s44298-026-00185-6

Figure Lengend Snippet: a Serum IgG and b IgA 50% endpoint antibody titers towards prefusion stabilized spike (S-trimer), the S1 and S2 subdomains of spike and the receptor binding domain (RBD) of the ancestral SARS-CoV-2 strain (Anc.) and delta, omicron BA.1 and BA.5 variants and the seasonal human coronaviruses (sHCoVs) 229E, NL63, HKU1 and OC43 were determined using protein microarray from healthy individuals sampled before (blue) or at the end of the pandemic (red). Mean titers are indicated on top and the number of seroconverted individuals is indicated below the graph. The lower limit of detection (LLoD) is indicated by a dotted line. c The frequency of reactive IgG and d IgA B-cells was determined by B-cell profiling for 8 donors sampled pre- (blue) and 8 donors sampled end-pandemic (red). Center line, median; boxes, upper and lower quartiles; significance of titer differences and B-cell frequencies for sHCoVs were calculated using a Mann-Whitney test and shown as * p < 0.05 or ** p < 0.01.

Article Snippet: The OC43 strain VR-1558 (ATCC, CCL-244) was propagated in MRC-5 cells (ATCC, CCL-171).

Techniques: Binding Assay, Microarray, MANN-WHITNEY

Journal: npj Viruses

Article Title: SARS-CoV-2 crossreactive B-cells outnumber seasonal coronavirus spike-specific clones at the end of the COVID-19 pandemic

doi: 10.1038/s44298-026-00185-6

Figure Lengend Snippet: a Type specific and cross-reactive clones targeting SARS-CoV-2 and alpha-CoV or SARS-CoV-2 and beta-CoV pre- and end-pandemic are enumerated and plotted in Venn diagrams for S-trimer, S1, and S2. b The fraction of clones that are SARS-CoV-2-specific (red), sHCoV-specific (blue), or cross-reactive (green) from pooled pre- and pooled end-pandemic donors is plotted. c Proportion of beta-CoV clones, including SARS-CoV-2, that are reactive towards S1 (orange) or S2 (dark red) pre- and end-pandemic. d The level of reactivity of SARS-CoV-2, 229E and NL63 S1-reactive (left panel) as well as SARS-CoV-2, HKU1 and OC43 S2-reactive IgG clones (right panel) detected in end-pandemic donors is indicated as the mean fluorescent intensity (MFI; range: 1,000-65,350). Cross-reactivity of clones is indicated by a connecting line.

Article Snippet: The OC43 strain VR-1558 (ATCC, CCL-244) was propagated in MRC-5 cells (ATCC, CCL-171).

Techniques: Clone Assay

Journal: npj Viruses

Article Title: SARS-CoV-2 crossreactive B-cells outnumber seasonal coronavirus spike-specific clones at the end of the COVID-19 pandemic

doi: 10.1038/s44298-026-00185-6

Figure Lengend Snippet: a Serum OC43 neutralization potential was determined using 50% focus reduction neutralization tests (FRNT50) and compared between pre- (blue) and end-pandemic (red) donors. Statistical significance was determined by Mann–Whitney U -test. b Linear regression analysis between serum OC43 neutralization titers and OC43 IgG binding titers (pre-pandemic, blue) or OC43 and SARS-CoV-2 IgG binding titers (end-pandemic, red). The regression line and 95% confidence interval are plotted for significant correlations ( p < 0.05). c The OC43 neutralization titers of sera depleted of OC43 S1-specific antibodies (left panel) (Wilcoxon paired t -test), and sera depleted for OC43 S2- or SARS-CoV-2 S2-specific antibodies (right panel), normalized to mock-depleted sera are shown. Statistical significance was determined between the original titers by one-way ANOVA with Geisser-Greenhouse correction. d IgG B-cell culture supernatants that were OC43 S1-specific, S2-specific, or SARS-CoV-2/OC43 S2-cross-reactive were tested in a OC43 focus reduction assay and compared with non-reactive controls (unpaired Student’s t-test). Significant differences were plotted as * p < 0.05, ** p < 0.01 and *** p < 0.001.

Article Snippet: The OC43 strain VR-1558 (ATCC, CCL-244) was propagated in MRC-5 cells (ATCC, CCL-171).

Techniques: Neutralization, MANN-WHITNEY, Binding Assay, Cell Culture

Journal: International Journal of Lipids

Article Title: Lipid Components in the Dynamin Fraction Prepared from Rat Brain

doi: 10.14302/issn.2835-513x.ijl-18-2122

Figure Lengend Snippet: Figure 3. Mass-spectrum of three OHCs (A; 7OHC, B; 24OHC, C; 27OHC) and peaks #1~#5

Article Snippet: 27-hydroxycholesterol (27OHC) was from Avanti Polar Lipids, Inc. 24(S)-hydroxycholesterol (24OHC) was obtained from Focus Biomolecules.

Techniques: