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Image Search Results
Journal: Emerging Microbes & Infections
Article Title: Optimized HepaRG is a suitable cell source to generate the human liver chimeric mouse model for the chronic hepatitis B virus infection
doi: 10.1038/s41426-018-0143-9
Figure Lengend Snippet: a Measurements of hALB in the supernatant of the culture medium and cell proliferation capacity of HepaRG cells with the FH1, FPH1, FPH2 and XMU-MP-1 treatment in the one-minus treatment pattern ( n = 4/group) for 1 (left panel) or 2 weeks (right panel). b FACS analysis for the hALB-positive rate of HepaRG cells treated or untreated with 4SM from weeks 0 to 4 in the hepatic differentiation procedure ( n = 4/group). c FACS analysis of the hALB and hAAT double-positive (DP) rate of cells using the hepatic differentiation procedure that was optimized by 4SM treatment and cell enrichment; udHepaRG and cdHepaRG cells were used as controls. d Representative FACS plot for developing DP cells using the 4SM-enhanced hepatic differentiation procedure with or without cell enrichment. (* P < 0.01; **** P < 0.00001; NS no significant difference, U.D. undetectable)
Article Snippet: The 4SM was purchased from
Techniques:
Journal: Emerging Microbes & Infections
Article Title: Optimized HepaRG is a suitable cell source to generate the human liver chimeric mouse model for the chronic hepatitis B virus infection
doi: 10.1038/s41426-018-0143-9
Figure Lengend Snippet: a Measurements of hALB in the supernatant of the culture medium and cell proliferation capacity of HepaRG cells with the FH1, FPH1, FPH2 and XMU-MP-1 treatment in the one-minus treatment pattern ( n = 4/group) for 1 (left panel) or 2 weeks (right panel). b FACS analysis for the hALB-positive rate of HepaRG cells treated or untreated with 4SM from weeks 0 to 4 in the hepatic differentiation procedure ( n = 4/group). c FACS analysis of the hALB and hAAT double-positive (DP) rate of cells using the hepatic differentiation procedure that was optimized by 4SM treatment and cell enrichment; udHepaRG and cdHepaRG cells were used as controls. d Representative FACS plot for developing DP cells using the 4SM-enhanced hepatic differentiation procedure with or without cell enrichment. (* P < 0.01; **** P < 0.00001; NS no significant difference, U.D. undetectable)
Article Snippet: The
Techniques:
Journal: Emerging Microbes & Infections
Article Title: Optimized HepaRG is a suitable cell source to generate the human liver chimeric mouse model for the chronic hepatitis B virus infection
doi: 10.1038/s41426-018-0143-9
Figure Lengend Snippet: a Investigation of the optimized 2-week hepatic differentiation procedure including 4SM treatment and enrichment of precursor HLCs (DP pdHepaRG). b Classic 4-week hepatic differentiation procedure with only DMSO treatment
Article Snippet: The
Techniques:
Journal: Emerging Microbes & Infections
Article Title: Optimized HepaRG is a suitable cell source to generate the human liver chimeric mouse model for the chronic hepatitis B virus infection
doi: 10.1038/s41426-018-0143-9
Figure Lengend Snippet: a Schematic diagram of the experimental plan for engraftment of HepaRG cells in different hepatic differentiation states to liver failure FRGS mice with or without 4SM treatment. FRGS mice without engraftment were used as a control. b ELISA assays to determine the serum hALB levels at weeks 0 to 24 post-engraftment ( n = 6/group). c FACS analysis to examine the ratio of hALB + cells in the liver of the engrafted FRGS mice with 4SM treatment at weeks 8 and 24 after engraftment. FRGS mice without cell engraftment were used as a control ( n = 6/group). d qRT-PCR to detect the mRNA levels of control (hGAPDH) or nine typical human hepatocyte-specific genes in hALB + cells from the engrafted FRGS mice treated with 4SM at week 8 and 24 after engraftment ( n = 3/group). e IHC to visualize hALB-positive cells in liver tissues from the engrafted FRGS mice with 4SM treatment at week 24 post-engraftment (bar = 200 μm). (* P < 0.01; ** P < 0.001; **** P < 0.00001; NS no significant difference, U.D. undetectable)
Article Snippet: The
Techniques: Enzyme-linked Immunosorbent Assay, Quantitative RT-PCR