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Image Search Results
Journal:
Article Title: A protein multiplex microarray substrate with high sensitivity and specificity
doi: 10.1016/j.jim.2010.10.005
Figure Lengend Snippet: 2- I. Microarrays spotted with two-fold serial dilutions of mouse monoclonal IgG were probed with Cy3-labelled goat anti-mouse IgG antibody and scanned using an Affymetrix 418 scanner. Slide C was scanned with 10% gain because of background. It should be noted that the FAST slide has the same nitrocellulose binding characteristics as the colloidal nitrocellulose but the higher background of the FAST slide, due to the thick layer, i.e. 10μm, of nitrocellulose, decreases the signal detection level. Slides B and D were scanned at 50 gain. A1 and A2 are the same slide scanned at 10 and 50 gain respectively, to obtain the full the detection and quantitation range.
Article Snippet: The processed multiplex microarrays were scanned with an
Techniques: Binding Assay, Quantitation Assay