Journal: eLife

Article Title: A high-resolution map of transcriptional repression

doi: 10.7554/elife.22767

Figure Lengend Snippet: Figure 3. RNAP2 is not required for chromatin accessibility of Ikaros target promoters. (A) Left: Western blotting for RNAP2 and TFIIB after 3 hr treatment with 1 uM triptolide (TPL). Tubulin is a loading control. 3 independent biological replicates. Right: Possible impact of RNAPII removal: promoters may remain nucleosome-free (top) or not (bottom). (B) RNAP2 ChIP-PCR, TFIIB ChIP-PCR and MNase-PCR at the indicated promoters following 4 hr of 1 mM triptolide or carrier (DMSO, grey). Mean ± SE, 3 independent biological replicates. Triptolide significantly reduced RNAP2 and TFIIB occupancy but did not significantly affect nucleosome occupancy at the Igll1 and Myc promoters. (C) As (B) with 4-OHT present during the last hour. Mean ± SE, 3 independent biological replicates. Triptolide did not significantly affect the ability of Ikaros to increase nucleosome occupancy at the Igll1 and Myc promoters. DOI: 10.7554/eLife.22767.011 The following source data and figure supplements are available for figure 3:

Article Snippet: We used antibodies to histone H3 (Abcam AB1791), acetylated histone H3 (Abcam AB47915), acetylated histone H4 (Millipore 06–866), RNAPII (Santa Cruz sc-899), TFIIB (Santa Cruz sc-225), CHD4 (Abcam AB72418), and tubulin (Sigma T9026).

Techniques: Western Blot, Control

Journal: Journal of visualized experiments : JoVE

Article Title: Reusable Single Cell for Iterative Epigenomic Analyses

doi: 10.3791/63456

Figure Lengend Snippet: Table 3

Article Snippet: Anhydrous DMF , Vector laboratories , S-4001-005 , Anhydrous N,N-dimethylformamide (DMF).

Techniques: Staining, Fluorescence, Microscopy, Plasmid Preparation, Bioburden Testing, Clone Assay, Ligation, Cell Counting, Spectrophotometry, Purification, Electron Microscopy, Transferring, Gel Extraction, Picogreen Assay, Recombinant

Journal: Environmental science. Nano

Article Title: Surface-controlled dissolution rates: a case study of nanoceria in carboxylic acid solutions

doi: 10.1039/C9EN00222G

Figure Lengend Snippet: Dissolution rate coefficients: controls and carboxylic acids.

Article Snippet: Materials The chemicals, their sources, purity, and CAS numbers were adipic acid, TCI, ≥ 99%, 124-04-9; ammonium nitrate, Fisher, ACS grade, 6484-52-2; citric acid monohydrate, Fisher, ACS grade, 5949-29-1; DL-3-hydroxybutyric acid sodium salt, Chem Impex Int’l Inc., 100.30%, 150-83-4 & 306-31-0; DL-malic acid, Alfa Aesar, 98%, 6915-15-7; glutaric acid, Acros organics, 99%,110-94-1; hydrogen peroxide 3% W/W, BDH chemicals, 7722-84-1; horseradish peroxide type II, Sigma, 150-250 U/mg, 9003-99-0; lactic acid, TCI, ≥ 85%, 50-21-5; pimelic acid, Alfa Aesar, 98+%, 111-16-0; sodium acetate, VWR, ACS grade, 127-09-3; sodium azide, Sigma, 99.8%, 26628-22-8; sodium nitrate, BDH chemicals, ACS grade, 7631-99-4; succinic acid, TCI America, ≥ 99%,110-15-6; and tricarballylic acid, Alfa Aesar, 98%, 99-14-9.

Techniques: Dissolution