Journal: bioRxiv

Article Title: Arc- Expressing Accessory Olfactory Bulb Interneurons Support Chemosensory Social Behavioral Plasticity

doi: 10.1101/2022.05.02.490296

Figure Lengend Snippet: Re-exposure to the same intruder induces Arc expression in a similar population of IGCs across multiple days. ( A ) Depiction of repeated resident-intruder behavioral paradigm involving triple transgenic (ArcTRAP x Ai9 x Arc-d4EGFP) mice. 4-OHT injection was paired with resident-intruder exposure on Day 0. On Day 3, resident mice were either exposed to the same male intruder seen in the previous days, or to a novel female intruder. ( B ) Left: AOB image from a resident exposed to the same male intruder on all 4 days. Right: AOB image from a resident exposed to the same male intruder for three days, and a novel female intruder on the fourth day. Top middle: ICL inset from male-male exposure. Bottom middle: ICL inset from male-female exposure. tdTomato (magenta) label induced by Arc expression on Day 0. GFP (cyan) label induced by Arc expression on Day 3. White indicates colocalization between tdTomato and EGFP. Dashed line outlines the ICL. Scale bars 100 µm. ( C ) Colocalization analyses for the fraction of tdTomato+ pixels (magenta) colocalized with eGFP (cyan). ( D ) Colocalization analysis for the fraction of eGFP pixels colocalized with tdTomato pixels. Unpaired Student’s t -test, *p=0.038, ****p<0.0001. n=16-19 AOB slices from 4-5 animals.

Article Snippet: The 4-OHT/corn oil solution was then loaded into insulin syringes (BD; Catalog #329461) at a dose of 50mg/kg for injection.

Techniques: Expressing, Transgenic Assay, Injection

Journal: bioRxiv

Article Title: Arc- Expressing Accessory Olfactory Bulb Interneurons Support Chemosensory Social Behavioral Plasticity

doi: 10.1101/2022.05.02.490296

Figure Lengend Snippet: TRAPing during resident-intruder interactions permanently labels AOB IGCs with tdTomato. ( A ) Depiction of the TRAPing process (paired 4-OHT injection and social interaction). Day 0 is “TRAP Day.” ( B-D ) Sample AOB images of tdTomato expression in ArcTRAP mice in “4-OHT+Intruder” ( B ), “4-OHT alone” ( C ), and “Intruder alone” ( D ) conditions. In all three images, the ICL is outlined by the dashed region to the left, and the external cellular layer and glomerular layer are outlined by the dashed region to the right. Scale bar: 100 µm. ( E ) Quantification of tdTomato-positive cells in the ICL. Cell counts are normalized to the ICL area. Error bars represent mean ± SD. One-way ANOVA, multiple comparisons **p=0.0059, ****p<0.0001. n=9-12 AOB sections from 3-4 mice per treatment.

Article Snippet: The 4-OHT/corn oil solution was then loaded into insulin syringes (BD; Catalog #329461) at a dose of 50mg/kg for injection.

Techniques: Injection, Expressing