3d10 Search Results


mouse anti dmpk  (Developmental Studies Hybridoma Bank)
94
Developmental Studies Hybridoma Bank mouse anti dmpk
Mouse Anti Dmpk, supplied by Developmental Studies Hybridoma Bank, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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ex5 3d10 igg2a  (ATCC)
90
ATCC ex5 3d10 igg2a
Ex5 3d10 Igg2a, supplied by ATCC, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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cbs  (Novus Biologicals)
90
Novus Biologicals cbs
Cbs, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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3d10  (Novus Biologicals)
94
Novus Biologicals 3d10
Fig. 1. IHC expression of WNT5A. A–E) Perinuclear, cytoplasmic, and in some cells nuclear immunostaining with the polyclonal AF645 antibody. F–T) Cytoplasmic staining of WNT5A with the monoclonal antibodies (F–I) 3A4, (K–N) <t>3D10,</t> and (P–S) 6F2. J) Lack of the WNT5A staining in hepatocytes of normal liver with the monoclonal 3A4 antibody. O) Cytoplasmic staining of WNT5A with the monoclonal 3D10 and 6F2 (T) antibodies. Scale bars = 50 µm.
3d10, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 94 stars, based on 1 article reviews
3d10 - by Bioz Stars, 2026-04
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gsk3β  (Novus Biologicals)
90
Novus Biologicals gsk3β
Fig. 1. IHC expression of WNT5A. A–E) Perinuclear, cytoplasmic, and in some cells nuclear immunostaining with the polyclonal AF645 antibody. F–T) Cytoplasmic staining of WNT5A with the monoclonal antibodies (F–I) 3A4, (K–N) <t>3D10,</t> and (P–S) 6F2. J) Lack of the WNT5A staining in hepatocytes of normal liver with the monoclonal 3A4 antibody. O) Cytoplasmic staining of WNT5A with the monoclonal 3D10 and 6F2 (T) antibodies. Scale bars = 50 µm.
Gsk3β, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anti gsk3β mouse monoclonal igg antibody  (Novus Biologicals)
93
Novus Biologicals anti gsk3β mouse monoclonal igg antibody
Fig. 5 | REST suppresses the tau kinases CDK5 and <t>GSK3β.</t> a, b Loss of REST in excitatory neurons increases CDK5 expression in cortex and hippocampus. aImmunolabelingforCDK5(green)andthe neuronalmarkerMAP2(magenta)in CA1 neurons of the hippocampus in 9-month-old 3xTg and 3xTg;cKO mice. b Quantification of CDK5 immunofluorescence intensity in the hippocampus and cortex of 9-month-old 3xTg (n = 4) and 3xTg;cKO (n = 4) mice. c, d Loss of a single REST allele increases CDK5 expression. c Immunolabeling for CDK5 (green) and MAP2 (magenta) in 29-month-old 3xTg and 3xTg;GT (heterozygous REST null) mice. d Quantification of CDK5 immunofluorescence intensity in 28–29-month-old 3xTg (n = 6) and 3xTg;GT (n = 6) mice. e, f Loss of REST in excitatory neurons increases GSKβ expression in cortex and hippocampus. e Immunolabeling for GSK3β (green) and MAP2 (magenta) in hippocampal CA1 neurons in 9-month-old 3xTg and 3xTg;cKO mice. f Quantification of GSK3β immunofluorescenceintensityin9-month-old3xTg(n = 4)and3xTg;cKO (n = 4) mice. g, h Loss of a single REST allele increases GSK3β expression.
Anti Gsk3β Mouse Monoclonal Igg Antibody, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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h00000875 d01p novus  (Novus Biologicals)
90
Novus Biologicals h00000875 d01p novus
Fig. 5 | REST suppresses the tau kinases CDK5 and <t>GSK3β.</t> a, b Loss of REST in excitatory neurons increases CDK5 expression in cortex and hippocampus. aImmunolabelingforCDK5(green)andthe neuronalmarkerMAP2(magenta)in CA1 neurons of the hippocampus in 9-month-old 3xTg and 3xTg;cKO mice. b Quantification of CDK5 immunofluorescence intensity in the hippocampus and cortex of 9-month-old 3xTg (n = 4) and 3xTg;cKO (n = 4) mice. c, d Loss of a single REST allele increases CDK5 expression. c Immunolabeling for CDK5 (green) and MAP2 (magenta) in 29-month-old 3xTg and 3xTg;GT (heterozygous REST null) mice. d Quantification of CDK5 immunofluorescence intensity in 28–29-month-old 3xTg (n = 6) and 3xTg;GT (n = 6) mice. e, f Loss of REST in excitatory neurons increases GSKβ expression in cortex and hippocampus. e Immunolabeling for GSK3β (green) and MAP2 (magenta) in hippocampal CA1 neurons in 9-month-old 3xTg and 3xTg;cKO mice. f Quantification of GSK3β immunofluorescenceintensityin9-month-old3xTg(n = 4)and3xTg;cKO (n = 4) mice. g, h Loss of a single REST allele increases GSK3β expression.
H00000875 D01p Novus, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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receptor 3  (Novus Biologicals)
90
Novus Biologicals receptor 3
Fig. 5 | REST suppresses the tau kinases CDK5 and <t>GSK3β.</t> a, b Loss of REST in excitatory neurons increases CDK5 expression in cortex and hippocampus. aImmunolabelingforCDK5(green)andthe neuronalmarkerMAP2(magenta)in CA1 neurons of the hippocampus in 9-month-old 3xTg and 3xTg;cKO mice. b Quantification of CDK5 immunofluorescence intensity in the hippocampus and cortex of 9-month-old 3xTg (n = 4) and 3xTg;cKO (n = 4) mice. c, d Loss of a single REST allele increases CDK5 expression. c Immunolabeling for CDK5 (green) and MAP2 (magenta) in 29-month-old 3xTg and 3xTg;GT (heterozygous REST null) mice. d Quantification of CDK5 immunofluorescence intensity in 28–29-month-old 3xTg (n = 6) and 3xTg;GT (n = 6) mice. e, f Loss of REST in excitatory neurons increases GSKβ expression in cortex and hippocampus. e Immunolabeling for GSK3β (green) and MAP2 (magenta) in hippocampal CA1 neurons in 9-month-old 3xTg and 3xTg;cKO mice. f Quantification of GSK3β immunofluorescenceintensityin9-month-old3xTg(n = 4)and3xTg;cKO (n = 4) mice. g, h Loss of a single REST allele increases GSK3β expression.
Receptor 3, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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313399 leaf purified anti mouse ly49h  (Bio X Cell)
92
Bio X Cell 313399 leaf purified anti mouse ly49h
(A) Histograms of <t>Ly49H</t> expression on splenic Ly49H+ NK cells (gating strategy in Figure S1A) from uninfected (UI) and MCMV-infected WT mice at day 7 PI (D7). Data are representative of at least five independent experiments with 5–10 mice per experiment.
313399 Leaf Purified Anti Mouse Ly49h, supplied by Bio X Cell, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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monoclonal anti epor antibody  (R&D Systems)
90
R&D Systems monoclonal anti epor antibody
(A) Histograms of <t>Ly49H</t> expression on splenic Ly49H+ NK cells (gating strategy in Figure S1A) from uninfected (UI) and MCMV-infected WT mice at day 7 PI (D7). Data are representative of at least five independent experiments with 5–10 mice per experiment.
Monoclonal Anti Epor Antibody, supplied by R&D Systems, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anti gsk3β antibody  (Novus Biologicals)
93
Novus Biologicals anti gsk3β antibody
(A) Histograms of <t>Ly49H</t> expression on splenic Ly49H+ NK cells (gating strategy in Figure S1A) from uninfected (UI) and MCMV-infected WT mice at day 7 PI (D7). Data are representative of at least five independent experiments with 5–10 mice per experiment.
Anti Gsk3β Antibody, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mouse anti human c ebpβ monoclonal  (Developmental Studies Hybridoma Bank)
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Developmental Studies Hybridoma Bank mouse anti human c ebpβ monoclonal

Mouse Anti Human C Ebpβ Monoclonal, supplied by Developmental Studies Hybridoma Bank, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Fig. 1. IHC expression of WNT5A. A–E) Perinuclear, cytoplasmic, and in some cells nuclear immunostaining with the polyclonal AF645 antibody. F–T) Cytoplasmic staining of WNT5A with the monoclonal antibodies (F–I) 3A4, (K–N) 3D10, and (P–S) 6F2. J) Lack of the WNT5A staining in hepatocytes of normal liver with the monoclonal 3A4 antibody. O) Cytoplasmic staining of WNT5A with the monoclonal 3D10 and 6F2 (T) antibodies. Scale bars = 50 µm.

Journal: Biotechnic & histochemistry : official publication of the Biological Stain Commission

Article Title: Optimization, validation, and identification of two reliable antibodies for immunodetection of WNT5A.

doi: 10.1080/10520295.2016.1255995

Figure Lengend Snippet: Fig. 1. IHC expression of WNT5A. A–E) Perinuclear, cytoplasmic, and in some cells nuclear immunostaining with the polyclonal AF645 antibody. F–T) Cytoplasmic staining of WNT5A with the monoclonal antibodies (F–I) 3A4, (K–N) 3D10, and (P–S) 6F2. J) Lack of the WNT5A staining in hepatocytes of normal liver with the monoclonal 3A4 antibody. O) Cytoplasmic staining of WNT5A with the monoclonal 3D10 and 6F2 (T) antibodies. Scale bars = 50 µm.

Article Snippet: Polyclonal goat anti-mouse/rat WNT5A (AF645) was purchased from R&D Systems, monoclonal mouse anti-human WNT5A clone 3A4 (H00007474M04) was purchased from Abnova Corp. (Taipei, Taiwan), monoclonal mouse anti-human WNT5A clone 3D10 (NBP1-47438) was purchased from Novus Biologicals (Littleton, CO), and monoclonal mouse anti-human WNT5A clone 6F2 (LS-B3859) was purchased from LifeSpan BioSciences (Seattle, WA).

Techniques: Expressing, Immunostaining, Staining, Bioprocessing

Fig. 2. Assessment of IHC using reducing western blot analysis. A) WNT5A was detected at 43 kDa in MDA-MB468 cell lysate supplemented with 4 ng/µl of rWNT5A and MDA-MB468 cells transfected with a WNT5A-containing vector, with the polyclonal AF645 antibody. B–D) No WNT5A protein band was detected with the monoclonal antibodies (B) 3A4, (C) 3D10 or (D) 6F2. α-Tubulin antibody was used as the loading control.

Journal: Biotechnic & histochemistry : official publication of the Biological Stain Commission

Article Title: Optimization, validation, and identification of two reliable antibodies for immunodetection of WNT5A.

doi: 10.1080/10520295.2016.1255995

Figure Lengend Snippet: Fig. 2. Assessment of IHC using reducing western blot analysis. A) WNT5A was detected at 43 kDa in MDA-MB468 cell lysate supplemented with 4 ng/µl of rWNT5A and MDA-MB468 cells transfected with a WNT5A-containing vector, with the polyclonal AF645 antibody. B–D) No WNT5A protein band was detected with the monoclonal antibodies (B) 3A4, (C) 3D10 or (D) 6F2. α-Tubulin antibody was used as the loading control.

Article Snippet: Polyclonal goat anti-mouse/rat WNT5A (AF645) was purchased from R&D Systems, monoclonal mouse anti-human WNT5A clone 3A4 (H00007474M04) was purchased from Abnova Corp. (Taipei, Taiwan), monoclonal mouse anti-human WNT5A clone 3D10 (NBP1-47438) was purchased from Novus Biologicals (Littleton, CO), and monoclonal mouse anti-human WNT5A clone 6F2 (LS-B3859) was purchased from LifeSpan BioSciences (Seattle, WA).

Techniques: Western Blot, Transfection, Plasmid Preparation, Bioprocessing, Control

Fig. 3. Assessment of IHC with native western blot analysis. A, B) Lack of the WNT5A protein band at 43 kDa in the MDA-MB468 cell lysate supplemented with 4 ng/µl of rWNT5A and the MDA-MB468 cell lysate transfected with a WNT5A-containing vector with two monoclonal antibodies (A) 3D10 and (B) 6F2.

Journal: Biotechnic & histochemistry : official publication of the Biological Stain Commission

Article Title: Optimization, validation, and identification of two reliable antibodies for immunodetection of WNT5A.

doi: 10.1080/10520295.2016.1255995

Figure Lengend Snippet: Fig. 3. Assessment of IHC with native western blot analysis. A, B) Lack of the WNT5A protein band at 43 kDa in the MDA-MB468 cell lysate supplemented with 4 ng/µl of rWNT5A and the MDA-MB468 cell lysate transfected with a WNT5A-containing vector with two monoclonal antibodies (A) 3D10 and (B) 6F2.

Article Snippet: Polyclonal goat anti-mouse/rat WNT5A (AF645) was purchased from R&D Systems, monoclonal mouse anti-human WNT5A clone 3A4 (H00007474M04) was purchased from Abnova Corp. (Taipei, Taiwan), monoclonal mouse anti-human WNT5A clone 3D10 (NBP1-47438) was purchased from Novus Biologicals (Littleton, CO), and monoclonal mouse anti-human WNT5A clone 6F2 (LS-B3859) was purchased from LifeSpan BioSciences (Seattle, WA).

Techniques: Western Blot, Transfection, Plasmid Preparation, Bioprocessing

Fig. 4. Detection of WNT5A with the purified 3D10 antibody. A) Cytoplasmic immunostaining of WNT5A protein with the purified 3D10 antibody in duct epithelial cells of normal breast tissue, breast cancer cells, and OSCC cells. B) Lack of WNT5A protein band at 43 kDa in the MDA-MB468 cell lysate supplemented with 4 ng/µl of rWNT5A and the MDA- MB468 cell lysate transfected with a WNT5A-containing vector with the purified monoclonal 3D10 antibody. α-Tubulin antibody was used as the loading control. Scale bars = 50 µm.

Journal: Biotechnic & histochemistry : official publication of the Biological Stain Commission

Article Title: Optimization, validation, and identification of two reliable antibodies for immunodetection of WNT5A.

doi: 10.1080/10520295.2016.1255995

Figure Lengend Snippet: Fig. 4. Detection of WNT5A with the purified 3D10 antibody. A) Cytoplasmic immunostaining of WNT5A protein with the purified 3D10 antibody in duct epithelial cells of normal breast tissue, breast cancer cells, and OSCC cells. B) Lack of WNT5A protein band at 43 kDa in the MDA-MB468 cell lysate supplemented with 4 ng/µl of rWNT5A and the MDA- MB468 cell lysate transfected with a WNT5A-containing vector with the purified monoclonal 3D10 antibody. α-Tubulin antibody was used as the loading control. Scale bars = 50 µm.

Article Snippet: Polyclonal goat anti-mouse/rat WNT5A (AF645) was purchased from R&D Systems, monoclonal mouse anti-human WNT5A clone 3A4 (H00007474M04) was purchased from Abnova Corp. (Taipei, Taiwan), monoclonal mouse anti-human WNT5A clone 3D10 (NBP1-47438) was purchased from Novus Biologicals (Littleton, CO), and monoclonal mouse anti-human WNT5A clone 6F2 (LS-B3859) was purchased from LifeSpan BioSciences (Seattle, WA).

Techniques: Immunostaining, Transfection, Plasmid Preparation, Control

Fig. 6. Pre-absorption test of the monoclonal antibodies in IHC. A, B) Intensity of the cytoplasmic WNT5A immunostaining in normal breast tissue with the monoclonal 3A4 antibody (A) was markedly reduced after pre-incubation with rWNT5A (B). C , D) The intensity of the cytoplasmic WNT5A staining in normal breast tissue with the monoclonal 3A4 antibody (C) was moderately decreased after pre-incubation with rWNT3A (D). E, F) The intensity of the cytoplasmic WNT5A staining in normal breast tissue with the purified monoclonal 3D10 antibody (E) was increased after pre-incubation with rWNT5A. Scale bars = 50 µm.

Journal: Biotechnic & histochemistry : official publication of the Biological Stain Commission

Article Title: Optimization, validation, and identification of two reliable antibodies for immunodetection of WNT5A.

doi: 10.1080/10520295.2016.1255995

Figure Lengend Snippet: Fig. 6. Pre-absorption test of the monoclonal antibodies in IHC. A, B) Intensity of the cytoplasmic WNT5A immunostaining in normal breast tissue with the monoclonal 3A4 antibody (A) was markedly reduced after pre-incubation with rWNT5A (B). C , D) The intensity of the cytoplasmic WNT5A staining in normal breast tissue with the monoclonal 3A4 antibody (C) was moderately decreased after pre-incubation with rWNT3A (D). E, F) The intensity of the cytoplasmic WNT5A staining in normal breast tissue with the purified monoclonal 3D10 antibody (E) was increased after pre-incubation with rWNT5A. Scale bars = 50 µm.

Article Snippet: Polyclonal goat anti-mouse/rat WNT5A (AF645) was purchased from R&D Systems, monoclonal mouse anti-human WNT5A clone 3A4 (H00007474M04) was purchased from Abnova Corp. (Taipei, Taiwan), monoclonal mouse anti-human WNT5A clone 3D10 (NBP1-47438) was purchased from Novus Biologicals (Littleton, CO), and monoclonal mouse anti-human WNT5A clone 6F2 (LS-B3859) was purchased from LifeSpan BioSciences (Seattle, WA).

Techniques: Bioprocessing, Immunostaining, Incubation, Staining

Fig. 5 | REST suppresses the tau kinases CDK5 and GSK3β. a, b Loss of REST in excitatory neurons increases CDK5 expression in cortex and hippocampus. aImmunolabelingforCDK5(green)andthe neuronalmarkerMAP2(magenta)in CA1 neurons of the hippocampus in 9-month-old 3xTg and 3xTg;cKO mice. b Quantification of CDK5 immunofluorescence intensity in the hippocampus and cortex of 9-month-old 3xTg (n = 4) and 3xTg;cKO (n = 4) mice. c, d Loss of a single REST allele increases CDK5 expression. c Immunolabeling for CDK5 (green) and MAP2 (magenta) in 29-month-old 3xTg and 3xTg;GT (heterozygous REST null) mice. d Quantification of CDK5 immunofluorescence intensity in 28–29-month-old 3xTg (n = 6) and 3xTg;GT (n = 6) mice. e, f Loss of REST in excitatory neurons increases GSKβ expression in cortex and hippocampus. e Immunolabeling for GSK3β (green) and MAP2 (magenta) in hippocampal CA1 neurons in 9-month-old 3xTg and 3xTg;cKO mice. f Quantification of GSK3β immunofluorescenceintensityin9-month-old3xTg(n = 4)and3xTg;cKO (n = 4) mice. g, h Loss of a single REST allele increases GSK3β expression.

Journal: Nature communications

Article Title: A neurodegeneration checkpoint mediated by REST protects against the onset of Alzheimer's disease.

doi: 10.1038/s41467-023-42704-6

Figure Lengend Snippet: Fig. 5 | REST suppresses the tau kinases CDK5 and GSK3β. a, b Loss of REST in excitatory neurons increases CDK5 expression in cortex and hippocampus. aImmunolabelingforCDK5(green)andthe neuronalmarkerMAP2(magenta)in CA1 neurons of the hippocampus in 9-month-old 3xTg and 3xTg;cKO mice. b Quantification of CDK5 immunofluorescence intensity in the hippocampus and cortex of 9-month-old 3xTg (n = 4) and 3xTg;cKO (n = 4) mice. c, d Loss of a single REST allele increases CDK5 expression. c Immunolabeling for CDK5 (green) and MAP2 (magenta) in 29-month-old 3xTg and 3xTg;GT (heterozygous REST null) mice. d Quantification of CDK5 immunofluorescence intensity in 28–29-month-old 3xTg (n = 6) and 3xTg;GT (n = 6) mice. e, f Loss of REST in excitatory neurons increases GSKβ expression in cortex and hippocampus. e Immunolabeling for GSK3β (green) and MAP2 (magenta) in hippocampal CA1 neurons in 9-month-old 3xTg and 3xTg;cKO mice. f Quantification of GSK3β immunofluorescenceintensityin9-month-old3xTg(n = 4)and3xTg;cKO (n = 4) mice. g, h Loss of a single REST allele increases GSK3β expression.

Article Snippet: Additional primary antibodies were as follows: anti-human Aβ rabbit monoclonal IgG antibody (Cell Signaling, Cat. No. 8243); antihuman APP mouse monoclonal IgG antibody (clone 6E10; Covance, Catalog No. SIG-39320); anti-actin mouse monoclonal IgG antibody (clone ACTN05 (C4); ThermoFisher Scientific, CatalogNo.MA5-11869); anti-NeuN mouse monoclonal IgG antibody (clone A60, Millipore, MAB377); anti-MAP2 goat polyclonal IgG antibody (PhosphoSolutions, Catalog. No. 1099-MAP2); anti-CDK5 mouse monoclonal IgG antibody (clone 4E4; Novus Bio, Catalog No. NBP2-37602); anti-GSK3β mouse monoclonal IgG antibody (clone D5C5Z; Novus Bio, catalog No. NBP147470S); anti-PS1 C-terminal (CTF) rabbit monoclonal IgG antibody (clone EP2000Y; Abcam, Catalog No. ab76083); anti-PS1 N-terminal (NTF) rabbit polyclonal IgG antibody (231-f; made in the Yankner lab); anti-Nicastrin mouse monoclonal IgG antibody (clone 9C3; Biolegend, Catalog No. 852301); anti-Nicastrin rabbit polyclonal IgG antibody (Sigma Millipore, Catalog No. N1660); anti-PEN2 rabbit polyclonal IgG antibody (ProScience, Catalog No.3981); anti-PEN2 rabbit monoclonal IgG antibody (clone EPR9200; Abcam, Catalog No. ab154830); antiPEN2 rabbit polyclonal IgG (ProScience, Catalog No. 3981); antiTransferrin receptor mouse monoclonal IgG antibody (clone H68.4; ThermoFisher Scientific, Catalog No. 13-6800); anti-BiP/GRP78 mouse monoclonal IgG (clone C38; ThermoFisher Scientific, clone C38, CatalogNo.

Techniques: Expressing, Immunolabeling

(A) Histograms of Ly49H expression on splenic Ly49H+ NK cells (gating strategy in Figure S1A) from uninfected (UI) and MCMV-infected WT mice at day 7 PI (D7). Data are representative of at least five independent experiments with 5–10 mice per experiment.

Journal: Immunity

Article Title: Cytomegalovirus infection drives avidity selection of natural killer cells

doi: 10.1016/j.immuni.2019.04.009

Figure Lengend Snippet: (A) Histograms of Ly49H expression on splenic Ly49H+ NK cells (gating strategy in Figure S1A) from uninfected (UI) and MCMV-infected WT mice at day 7 PI (D7). Data are representative of at least five independent experiments with 5–10 mice per experiment.

Article Snippet: ​ REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-Mouse CD3ε (clone 17A2) Tonbo Biosciences Cat#25–0032; RRID:AB_2621619 Anti-Mouse TCRβ (clone H57–597) BioLegend Cat#109220; RRID:AB_893624 Anti-Mouse CD19 (clone 6D5) BioLegend Cat#115530; RRID:AB_830707 Anti-Mouse F4/80 (clone BM8.1) BioLegend Cat#123117; RRID:AB_893489 Anti-Mouse NK1.1 (clone PK136) for flow cytometry Tonbo Biosciences Cat#65–5941; RRID:AB_2621910 Anti-Mouse Ly49H (clone 3D10) for flow cytometry eBioscience Cat#11-5886-81; RRID:AB_1257160 Anti-Mouse CD45.1 (clone A20) BioLegend Cat#110729; RRID:AB_1134170 Anti-Mouse CD45.2 (clone 104) BioLegend Cat#109821; RRID:AB_493730 Anti-Mouse/Human CD11b (clone M1/70) BioLegend Cat#101223; RRID:AB_755985 Anti-CD27 (clone LG.7F9) eBioscience Cat#14-0271-81; RRID:AB_467182 Anti-Mouse KLRG1 (clone 2F1) Tonbo Biosciences Cat#50–5893; RRID:AB_2621800 Anti-Mouse Ly49D (clone 4E5) BioLegend Cat#138308; RRID:AB_10639939 Anti-Mouse Ly49C and Ly49I (clone 5E6) BD Biosciences Cat#553277; RRID:AB_394751 Anti-Mouse NKG2A/C/E (clone 20D5) BD Biosciences Cat#740549; RRID:AB_2740251 Anti-Mouse CD25 (clone PC61) BioLegend Cat#102016; RRID:AB_312865 Anti-Mouse IFN gamma (clone XMG1.2) Tonbo Biosciences Cat#20–7311; RRID:AB_2621616 Anti-Mouse CD107a (clone 1D4B) BioLegend Cat#121611; RRID:AB_1732051 Anti-Stat4 pY693 (clone 38/p-Stat4) BD Biosciences Cat#558137; RRID:AB_397052 Anti-Mouse CD218a (IL-18Ra) (clone P3TUNYA) eBioscience Cat#48-5183-80; RRID:AB_2574068 Anti-Human CD3 (clone UCHT1) BD Biosciences Cat#563852; RRID:AB_2744391 Anti-Human CD56 (clone N901) Beckman Coulter Cat# {"type":"entrez-protein","attrs":{"text":"A82943","term_id":"11356850","term_text":"pir||A82943"}} A82943 ; RRID:AB_2750853 Anti-Human CD159c (NKG2C) (clone REA205) Miltenyi Cat#130-117-398; RRID:AB_2727933 InVivo Mab Anti-Mouse CD8α (clone 2.43) for NK cell enrichment Bio X Cell Cat#BE0061; RRID:AB_1125541 InVivo Mab Anti-Mouse CD4 (clone GK1.5) for NK cell enrichment Bio X Cell Cat#BE0003–1; RRID:AB_1107636 InVivo Mab Anti-Mouse CD19 (clone 1D3) for NK cell enrichment Bio X Cell Cat#BE0150; RRID:AB_10949187 InVivo Mab Anti-Mouse Ter-119 (clone TER-119) for NK cell enrichment Bio X Cell Cat#BE0183; RRID:AB_10949625 LEAF™ Purified Anti-Mouse NK-1.1 (clone PK136) for plate-bound stimulation BioLegend Cat#108712; RRID:AB_ 313399 LEAF™ Purified Anti-Mouse Ly49H (clone 3D10) for plate-bound stimulation BioLegend Cat#144704; RRID:AB_ 2561551 Bacterial and Virus Strains Mouse Cytomegalovirus (MCMV) J.

Techniques: Expressing, Infection

(A-D) Experimental schematic (A). Equal numbers of splenic Ly49Hlo and Ly49Hhi NK cells, purified from congenically distinct WT mice, were co-transferred into Klra8−/− mice, and their responses tracked following MCMV infection of the recipients. (B) Flow plots gated on NK cells from blood at day 7 PI (left) and spleen at day 28 PI (middle). Quantification of percent Ly49Hlo and Ly49Hhi NK cells within total NK cells in blood at indicated days PI (right). (C) The percentage of Ly49Hlo and Ly49Hhi NK cells within transferred Ly49H+ NK cells in blood at indicated days PI (left) and in indicated organs at day 28 PI (right). (D) Histograms of Ly49H expression on Ly49Hlo and Ly49Hhi NK cells from blood at day 7 PI. Data are representative of two independent experiments with 3–5 mice per experiment.

Journal: Immunity

Article Title: Cytomegalovirus infection drives avidity selection of natural killer cells

doi: 10.1016/j.immuni.2019.04.009

Figure Lengend Snippet: (A-D) Experimental schematic (A). Equal numbers of splenic Ly49Hlo and Ly49Hhi NK cells, purified from congenically distinct WT mice, were co-transferred into Klra8−/− mice, and their responses tracked following MCMV infection of the recipients. (B) Flow plots gated on NK cells from blood at day 7 PI (left) and spleen at day 28 PI (middle). Quantification of percent Ly49Hlo and Ly49Hhi NK cells within total NK cells in blood at indicated days PI (right). (C) The percentage of Ly49Hlo and Ly49Hhi NK cells within transferred Ly49H+ NK cells in blood at indicated days PI (left) and in indicated organs at day 28 PI (right). (D) Histograms of Ly49H expression on Ly49Hlo and Ly49Hhi NK cells from blood at day 7 PI. Data are representative of two independent experiments with 3–5 mice per experiment.

Article Snippet: ​ REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-Mouse CD3ε (clone 17A2) Tonbo Biosciences Cat#25–0032; RRID:AB_2621619 Anti-Mouse TCRβ (clone H57–597) BioLegend Cat#109220; RRID:AB_893624 Anti-Mouse CD19 (clone 6D5) BioLegend Cat#115530; RRID:AB_830707 Anti-Mouse F4/80 (clone BM8.1) BioLegend Cat#123117; RRID:AB_893489 Anti-Mouse NK1.1 (clone PK136) for flow cytometry Tonbo Biosciences Cat#65–5941; RRID:AB_2621910 Anti-Mouse Ly49H (clone 3D10) for flow cytometry eBioscience Cat#11-5886-81; RRID:AB_1257160 Anti-Mouse CD45.1 (clone A20) BioLegend Cat#110729; RRID:AB_1134170 Anti-Mouse CD45.2 (clone 104) BioLegend Cat#109821; RRID:AB_493730 Anti-Mouse/Human CD11b (clone M1/70) BioLegend Cat#101223; RRID:AB_755985 Anti-CD27 (clone LG.7F9) eBioscience Cat#14-0271-81; RRID:AB_467182 Anti-Mouse KLRG1 (clone 2F1) Tonbo Biosciences Cat#50–5893; RRID:AB_2621800 Anti-Mouse Ly49D (clone 4E5) BioLegend Cat#138308; RRID:AB_10639939 Anti-Mouse Ly49C and Ly49I (clone 5E6) BD Biosciences Cat#553277; RRID:AB_394751 Anti-Mouse NKG2A/C/E (clone 20D5) BD Biosciences Cat#740549; RRID:AB_2740251 Anti-Mouse CD25 (clone PC61) BioLegend Cat#102016; RRID:AB_312865 Anti-Mouse IFN gamma (clone XMG1.2) Tonbo Biosciences Cat#20–7311; RRID:AB_2621616 Anti-Mouse CD107a (clone 1D4B) BioLegend Cat#121611; RRID:AB_1732051 Anti-Stat4 pY693 (clone 38/p-Stat4) BD Biosciences Cat#558137; RRID:AB_397052 Anti-Mouse CD218a (IL-18Ra) (clone P3TUNYA) eBioscience Cat#48-5183-80; RRID:AB_2574068 Anti-Human CD3 (clone UCHT1) BD Biosciences Cat#563852; RRID:AB_2744391 Anti-Human CD56 (clone N901) Beckman Coulter Cat# {"type":"entrez-protein","attrs":{"text":"A82943","term_id":"11356850","term_text":"pir||A82943"}} A82943 ; RRID:AB_2750853 Anti-Human CD159c (NKG2C) (clone REA205) Miltenyi Cat#130-117-398; RRID:AB_2727933 InVivo Mab Anti-Mouse CD8α (clone 2.43) for NK cell enrichment Bio X Cell Cat#BE0061; RRID:AB_1125541 InVivo Mab Anti-Mouse CD4 (clone GK1.5) for NK cell enrichment Bio X Cell Cat#BE0003–1; RRID:AB_1107636 InVivo Mab Anti-Mouse CD19 (clone 1D3) for NK cell enrichment Bio X Cell Cat#BE0150; RRID:AB_10949187 InVivo Mab Anti-Mouse Ter-119 (clone TER-119) for NK cell enrichment Bio X Cell Cat#BE0183; RRID:AB_10949625 LEAF™ Purified Anti-Mouse NK-1.1 (clone PK136) for plate-bound stimulation BioLegend Cat#108712; RRID:AB_ 313399 LEAF™ Purified Anti-Mouse Ly49H (clone 3D10) for plate-bound stimulation BioLegend Cat#144704; RRID:AB_ 2561551 Bacterial and Virus Strains Mouse Cytomegalovirus (MCMV) J.

Techniques: Purification, Infection, Expressing

KEY RESOURCES TABLE

Journal: Immunity

Article Title: Cytomegalovirus infection drives avidity selection of natural killer cells

doi: 10.1016/j.immuni.2019.04.009

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: ​ REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-Mouse CD3ε (clone 17A2) Tonbo Biosciences Cat#25–0032; RRID:AB_2621619 Anti-Mouse TCRβ (clone H57–597) BioLegend Cat#109220; RRID:AB_893624 Anti-Mouse CD19 (clone 6D5) BioLegend Cat#115530; RRID:AB_830707 Anti-Mouse F4/80 (clone BM8.1) BioLegend Cat#123117; RRID:AB_893489 Anti-Mouse NK1.1 (clone PK136) for flow cytometry Tonbo Biosciences Cat#65–5941; RRID:AB_2621910 Anti-Mouse Ly49H (clone 3D10) for flow cytometry eBioscience Cat#11-5886-81; RRID:AB_1257160 Anti-Mouse CD45.1 (clone A20) BioLegend Cat#110729; RRID:AB_1134170 Anti-Mouse CD45.2 (clone 104) BioLegend Cat#109821; RRID:AB_493730 Anti-Mouse/Human CD11b (clone M1/70) BioLegend Cat#101223; RRID:AB_755985 Anti-CD27 (clone LG.7F9) eBioscience Cat#14-0271-81; RRID:AB_467182 Anti-Mouse KLRG1 (clone 2F1) Tonbo Biosciences Cat#50–5893; RRID:AB_2621800 Anti-Mouse Ly49D (clone 4E5) BioLegend Cat#138308; RRID:AB_10639939 Anti-Mouse Ly49C and Ly49I (clone 5E6) BD Biosciences Cat#553277; RRID:AB_394751 Anti-Mouse NKG2A/C/E (clone 20D5) BD Biosciences Cat#740549; RRID:AB_2740251 Anti-Mouse CD25 (clone PC61) BioLegend Cat#102016; RRID:AB_312865 Anti-Mouse IFN gamma (clone XMG1.2) Tonbo Biosciences Cat#20–7311; RRID:AB_2621616 Anti-Mouse CD107a (clone 1D4B) BioLegend Cat#121611; RRID:AB_1732051 Anti-Stat4 pY693 (clone 38/p-Stat4) BD Biosciences Cat#558137; RRID:AB_397052 Anti-Mouse CD218a (IL-18Ra) (clone P3TUNYA) eBioscience Cat#48-5183-80; RRID:AB_2574068 Anti-Human CD3 (clone UCHT1) BD Biosciences Cat#563852; RRID:AB_2744391 Anti-Human CD56 (clone N901) Beckman Coulter Cat# {"type":"entrez-protein","attrs":{"text":"A82943","term_id":"11356850","term_text":"pir||A82943"}} A82943 ; RRID:AB_2750853 Anti-Human CD159c (NKG2C) (clone REA205) Miltenyi Cat#130-117-398; RRID:AB_2727933 InVivo Mab Anti-Mouse CD8α (clone 2.43) for NK cell enrichment Bio X Cell Cat#BE0061; RRID:AB_1125541 InVivo Mab Anti-Mouse CD4 (clone GK1.5) for NK cell enrichment Bio X Cell Cat#BE0003–1; RRID:AB_1107636 InVivo Mab Anti-Mouse CD19 (clone 1D3) for NK cell enrichment Bio X Cell Cat#BE0150; RRID:AB_10949187 InVivo Mab Anti-Mouse Ter-119 (clone TER-119) for NK cell enrichment Bio X Cell Cat#BE0183; RRID:AB_10949625 LEAF™ Purified Anti-Mouse NK-1.1 (clone PK136) for plate-bound stimulation BioLegend Cat#108712; RRID:AB_ 313399 LEAF™ Purified Anti-Mouse Ly49H (clone 3D10) for plate-bound stimulation BioLegend Cat#144704; RRID:AB_ 2561551 Bacterial and Virus Strains Mouse Cytomegalovirus (MCMV) J.

Techniques: Flow Cytometry, Purification, Recombinant, Staining, RNA Sequencing Assay, Expressing, Derivative Assay, Software, Imaging

Journal: eLife

Article Title: FABP4-mediated lipid accumulation and lipolysis in tumor-associated macrophages promote breast cancer metastasis

doi: 10.7554/eLife.101221

Figure Lengend Snippet:

Article Snippet: Antibody , Mouse anti-human C/EBPβ monoclonal , DSHB , Cat#PCRP-CEBPB-3D10 , 1:300.

Techniques: Concentration Assay, Staining, Saline, SYBR Green Assay, Red Blood Cell Lysis, Cell Culture, XF Assay, Reverse Transcription, Polymer, Plasmid Preparation, Blocking Assay, Enzyme-linked Immunosorbent Assay, Sequencing, Transfection, Recombinant, Microarray, Gene Expression, Expressing, Derivative Assay, Real-time Polymerase Chain Reaction, Software, Sterility, Migration, Pore Size, Flow Cytometry, Quantitative RT-PCR, Imaging, Microscopy, Transmission Assay, Electron Microscopy