Journal: Nature Communications

Article Title: Scar matrix drives Piezo1 mediated stromal inflammation leading to placenta accreta spectrum

doi: 10.1038/s41467-024-52351-0

Figure Lengend Snippet: A Heatmap showing significant differential ligand encoding genes expressed in dESFs on Scar and Physio matrices. B Representative IL-8 immunofluorescence images of dESFs treated with protein transport inhibitor GolgiStop for 6 h on Physio and Scar matrices, quantification shown in right panel; n = 49 and 53 cells for Physio and Scar, respectively. p = 5 × 10 −11 . C , D ELISA based analysis of IL-8 and G-CSF concentration in supernatant of dESFs on Physio and Scar in 3D and 2D; n = 3 samples. p = 0.0007 in ( C ) and p = 0.04 and 2 × 10 −5 in ( D ). E ELISA based measurement of IL-8 concentration in supernatant of dESFs treated overnight with DMSO, or 100 ng/ml TNFα; n = 3 samples. p = 0.0097. F Experimental workflow to test migration of HTR8 in medium conditioned from dESFs with gene silenced for IL-8 and G-CSF encoding genes, CXCL8 and CSF3, respectively. G Migration trajectories (initial location (x, y = 0,0)) of HTR8 conditioned with medium from dESFs silenced for CXCL8 and CSF3 genes, without or with addition of recombinant human (rh) IL-8 and G-CSF; Quantification of averaged velocities over 24 h shown in ( H ); p = 1×10 −8 , 8×10 −7 , 8×10 −9 , and 6 × 10 −5 ; n listed below each condition. I 3D chemotaxis of primary EVTs in collagen gel towards IL-8 and G-CSF gradient; Shown is a representative image of EVTs in collagen gel (left); Trajectories of individually tracked EVTs from their initial location (0,0) (middle and right); Cell trajectory with mean displacement towards cytokine end are labeled red, and counted ( n ); p value showing Rayleigh test of cell trajectories: p < 0.05 is considered chemotaxis. J ANSIA-based stromal invasion analysis of HTR8 in monolayer of dESFs silenced for CXCL8 and CSF3 genes; Control refers to scrambled sgRNA. p = 0.003 and 0.005. Data in figures B–E, H and J are showing as mean ± s.d.; statistical significance is determined by unpaired two-tailed t-test (* p < 0.05, ** p < 0.01, *** p < 0.001, and **** p < 0.0001). Source data are provided as a Source Data file. Figure 3F created with BioRender.com released under a Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International license ( https://creativecommons.org/licenses/by-nc-nd/4.0/deed.en ).

Article Snippet: After 4 days of decidualization, HTR8 spheroids prepared as aforementioned were suspended in 1 mg/ml collagen solution and plated on the 3D decidualization ESFs and incubated at 37 °C for 1 h to allow spheroid settle down and gel formation. dESF monolayer and HTR8 nuclear locations were recorded by Zeiss Apotome 3D scanning.

Techniques: Immunofluorescence, Enzyme-linked Immunosorbent Assay, Concentration Assay, Migration, Recombinant, Chemotaxis Assay, Labeling, Control, Two Tailed Test

Journal: European Journal of Radiology Open

Article Title: Reduced dose helical CT scout imaging on next generation wide volume CT system decreases scan length and overall radiation exposure

doi: 10.1016/j.ejro.2024.100578

Figure Lengend Snippet: Traditional and 3D Landmark scout images and scan start/end positions for a chest CT of a 54-year old female. (a) Traditional AP and lateral scout images and start and end scan positions. Lines A and D, and corresponding axial images represent the actual start and end positions of the CT scan planned using traditional scout images where the radiographer estimates the organ locations. Lines B and C and corresponding axial images represent the proper start and end positions for the chest CT to start above the lung apex and go through both adrenal glands. (b) Axial ultra-low dose CT images generated by 3D Landmark scouting and corresponding coronal and lateral projections from the same patient. E and F represent the appropriate start and end positions of the CT planned using the axial 3D Landmark images resulting in a decreased scan length of 34 mm compared to prior imaging (panel a) planned using traditional scout imaging (325 vs 291 mm, respectively). Correspondingly, the radiation dose of the chest CT scan planned by 3D landmark was reduced by 12 % compared to her prior scan planned with traditional scout imaging (249.8 mGy·cm vs 283.7 mGy·cm), representing a 0.47 mSv effective dose savings. Scout image radiation dose itself was reduced by 48 % utilizing 3D Landmark (16.67 vs. 8.67 mGy·cm).

Article Snippet: 61 consecutive patients with prior CT examination of the same body region that underwent clinical CT planned using 3D Landmark Scan and ALD (Canon Medical Aquilion ONE Insight Edition; Otawara, Tochigi, Japan) at a single center over a 3-month period from 7/17/23–10/16/23 were included in this study.

Techniques: Computed Tomography, Generated, Imaging

Journal: European Journal of Radiology Open

Article Title: Reduced dose helical CT scout imaging on next generation wide volume CT system decreases scan length and overall radiation exposure

doi: 10.1016/j.ejro.2024.100578

Figure Lengend Snippet: Demographics of 61 patients undergoing a total of 104 CT examinations. Weight and BMI is presented for patients at time of prior CT examination planned with traditional scout imaging (“prior”) and at time of recent CT examination planned with 3D Landmark and ALD (“new”).

Article Snippet: 61 consecutive patients with prior CT examination of the same body region that underwent clinical CT planned using 3D Landmark Scan and ALD (Canon Medical Aquilion ONE Insight Edition; Otawara, Tochigi, Japan) at a single center over a 3-month period from 7/17/23–10/16/23 were included in this study.

Techniques: Imaging, Standard Deviation

Journal: European Journal of Radiology Open

Article Title: Reduced dose helical CT scout imaging on next generation wide volume CT system decreases scan length and overall radiation exposure

doi: 10.1016/j.ejro.2024.100578

Figure Lengend Snippet: Scan lengths and radiation doses for 104 CT examinations planned using traditional scout imaging (“prior”) and planned using 3D Landmark and ALD (“new”). Radiation doses presented as dose length product (DLP), effective dose (in milisieverts), and CT dose index (CTDI). Total DLP and CTDI reflect the sum of the scout imaging dose and acquisition dose. Scan lengths, DLP and corresponding effective doses of diagnostic acquisitions are further broken into subgroup by body region. Effective radiation dose in millisieverts (mSv) was calculated from the DLP using a k factor of 0.014 for chest, 0.015 for abdomen, and 0.0145 for chest/abdomen/pelvis [13] . Data represented as median and interquartile range. n = 50 for chest CT scan length, n = 16 for abdomen scan length, n = 38 for chest/abdomen/pelvis scan length, and n = 104 for scout scan length. n = 49 for chest radiation doses, n = 15 for abdomen radiation doses, n = 36 for chest/abdomen/pelvis radiation doses, and n = 100 for scout radiation doses.

Article Snippet: 61 consecutive patients with prior CT examination of the same body region that underwent clinical CT planned using 3D Landmark Scan and ALD (Canon Medical Aquilion ONE Insight Edition; Otawara, Tochigi, Japan) at a single center over a 3-month period from 7/17/23–10/16/23 were included in this study.

Techniques: Imaging, Diagnostic Assay, Computed Tomography

Journal: European Journal of Radiology Open

Article Title: Reduced dose helical CT scout imaging on next generation wide volume CT system decreases scan length and overall radiation exposure

doi: 10.1016/j.ejro.2024.100578

Figure Lengend Snippet: Dose-length product (DLP) (mGy·cm) for (a) chest, (b) abdomen, and (c) chest/abdomen/pelvis CT exams planned using 3D Landmark Scan and Anatomic Landmark Detection and performed on next generation CT (“new DLP”) versus those planned using traditional scout methods (“prior DLP”). (a) Median DLP for chest CT was reduced by 11.9 %, from 182.1 (IQR 146.1–283.7) to 160.4 (IQR 133.8–223.6) mGy·cm, p = 0.007, n = 49. (b). Median DLP for abdomen CT was reduced by 47.3 % from 236.8 (IQR 178.1–284.60) to 124.9 (96.8–166.8) mGy·cm, p = 0.004, n = 15. (c) Median DLP for chest/abdomen/pelvis CT was reduced by 28.6 %, from 531.6 (IQR 465.4–689.2) to 379.7 (IQR 319.0–531.8) mGy·cm, respectively, p = 0.001, n = 36.

Article Snippet: 61 consecutive patients with prior CT examination of the same body region that underwent clinical CT planned using 3D Landmark Scan and ALD (Canon Medical Aquilion ONE Insight Edition; Otawara, Tochigi, Japan) at a single center over a 3-month period from 7/17/23–10/16/23 were included in this study.

Techniques:

Journal: European Journal of Radiology Open

Article Title: Reduced dose helical CT scout imaging on next generation wide volume CT system decreases scan length and overall radiation exposure

doi: 10.1016/j.ejro.2024.100578

Figure Lengend Snippet: Scan length (mm) for (a) chest, (b), abdomen, and (c) chest/abdomen/pelvis CT exams planned using 3D Landmark Scan and Anatomic Landmark Detection versus those planned using traditional scout images. (a) Median scan length 317.3 (IQR 302.3–338)mm compared to 330 (IQR 325.8–360)mm, respectively (p < 0.001, n = 50). Median reduction of 12.7 mm represented by the black dashed line. (b) Median scan length 235.5 (IQR 209.5–246)mm compared to 250 (IQR 240.1–260)mm respectively(p = 0.003, n = 16). Median reduction of 14.5 mm represented by the black line. (c) Median scan length 635 (IQR 625–667.3)mm compared to 660 (IQR 641.3–687.3)mm respectively (p < 0.001, n = 38). Median reduction of 25.0 mm represented by the black line.

Article Snippet: 61 consecutive patients with prior CT examination of the same body region that underwent clinical CT planned using 3D Landmark Scan and ALD (Canon Medical Aquilion ONE Insight Edition; Otawara, Tochigi, Japan) at a single center over a 3-month period from 7/17/23–10/16/23 were included in this study.

Techniques: