Journal: Nature Communications

Article Title: An injectable hydrogel enhances tissue repair after spinal cord injury by promoting extracellular matrix remodeling

doi: 10.1038/s41467-017-00583-8

Figure Lengend Snippet: MMP-9 mediates fibrotic ECM remodeling. a Gelatinase activity of MMP-9 and MMP-2 in the PBS ( N = 5) and the I-5 group ( N = 4) examined by zymography. b , c Graphs showing quantification of MMP-2 ( b ) and MMP-9 ( c ) activity. ** indicates p < 0.01 by two-tailed Student’s t -test. Error bars represent the SEM. d , e Representative images of transverse spinal cord sections from animals injected with either PBS ( d ) or I-5 ( e ). Asterisks indicate cystic cavities. A yellow dotted line indicates the boundary of the FN-rich matrix. The boxed regions in the middle panels were magnified in dʹ, eʹ. Scale bars represent 100 µm. f MMP-9 immunoreactive granules were bounded by CD11b positive membrane-like circular structures. Scale bars represent 50 µm. g , j Representative images of transverse spinal cord sections stained with eriochrome cyanine and eosin from animals with I-5 mixed with non-targeting control siRNA (NC) ( g ) or MMP-9 siRNA ( j ). Adjacent sections were stained with antibodies against FN ( h , k ). Scale bars represent 200 μm. i , l 3D reconstruction of the spinal cord tissue using the Neurolucida software. Scale bars represent 1 mm. Asterisks indicate cystic cavities. m Quantification graph of cavity volume. ** indicates p < 0.01 by two-tailed Student’s t -test. N = 5 for each group. Error bars represent the SEM

Article Snippet: Three-dimensional reconstruction of the lesion cavity was done using the Neurolucida tracing software equipped with the 3D Slide Scanning Module (MBF bioscience).

Techniques: Activity Assay, Zymography, Two Tailed Test, Injection, Membrane, Staining, Control, Software

Journal: Nature Communications

Article Title: An injectable hydrogel enhances tissue repair after spinal cord injury by promoting extracellular matrix remodeling

doi: 10.1038/s41467-017-00583-8

Figure Lengend Snippet: Interaction between macrophages and I-5 hydrogel. a Representative images of Nile Red fluorescence in a macrophage cell line. Cultured macrophages were treated with Nile Red (NR) alone or nanoparticles consisting of NR and either CP-2 or I-5. In addition, JNJ7777120, a histamine receptor 4 inhibitor, or mepyramine maleate, a histamine receptor 1 inhibitor, was added to the culture medium 30 min before treatment with I-5 polymer nanoparticles. Scale bar represents 50 µm. b Graph showing quantification of NR fluorescence intensity. *** indicates p < 0.001 by one-way ANOVA followed by Tukey’s post hoc analysis. N = 4 replicate experiments per group. Error bars represent the SEM. c , d Representative images of transverse spinal cord sections from animals injected with CP-2 hydrogel lacking the imidazole group. c Eriochrome cyanine and eosin staining revealed prominent cystic cavities (*) at the center of the lesion ( c ). d FN staining showed a smaller area of FN-rich matrix. Scale bars represent 200 μm. e 3D reconstruction of the spinal cord tissue from an animal injected with CP-2 hydrogel using the Neurolucida software. Scale bar represents 1 mm. f Graph showing the quantification of the cavity volumes. The data set for the I-5 group was the same as the one used in Fig. . ** indicates p < 0.01 by two-tailed Student’s t -test. N = 8 for the I-5 group and N = 5 for the CP-2 group. Error bars represent the SEM

Article Snippet: Three-dimensional reconstruction of the lesion cavity was done using the Neurolucida tracing software equipped with the 3D Slide Scanning Module (MBF bioscience).

Techniques: Fluorescence, Cell Culture, Polymer, Injection, Staining, Software, Two Tailed Test

Journal: European Journal of Radiology Open

Article Title: Reduced dose helical CT scout imaging on next generation wide volume CT system decreases scan length and overall radiation exposure

doi: 10.1016/j.ejro.2024.100578

Figure Lengend Snippet: Traditional and 3D Landmark scout images and scan start/end positions for a chest CT of a 54-year old female. (a) Traditional AP and lateral scout images and start and end scan positions. Lines A and D, and corresponding axial images represent the actual start and end positions of the CT scan planned using traditional scout images where the radiographer estimates the organ locations. Lines B and C and corresponding axial images represent the proper start and end positions for the chest CT to start above the lung apex and go through both adrenal glands. (b) Axial ultra-low dose CT images generated by 3D Landmark scouting and corresponding coronal and lateral projections from the same patient. E and F represent the appropriate start and end positions of the CT planned using the axial 3D Landmark images resulting in a decreased scan length of 34 mm compared to prior imaging (panel a) planned using traditional scout imaging (325 vs 291 mm, respectively). Correspondingly, the radiation dose of the chest CT scan planned by 3D landmark was reduced by 12 % compared to her prior scan planned with traditional scout imaging (249.8 mGy·cm vs 283.7 mGy·cm), representing a 0.47 mSv effective dose savings. Scout image radiation dose itself was reduced by 48 % utilizing 3D Landmark (16.67 vs. 8.67 mGy·cm).

Article Snippet: 61 consecutive patients with prior CT examination of the same body region that underwent clinical CT planned using 3D Landmark Scan and ALD (Canon Medical Aquilion ONE Insight Edition; Otawara, Tochigi, Japan) at a single center over a 3-month period from 7/17/23–10/16/23 were included in this study.

Techniques: Computed Tomography, Generated, Imaging

Journal: European Journal of Radiology Open

Article Title: Reduced dose helical CT scout imaging on next generation wide volume CT system decreases scan length and overall radiation exposure

doi: 10.1016/j.ejro.2024.100578

Figure Lengend Snippet: Demographics of 61 patients undergoing a total of 104 CT examinations. Weight and BMI is presented for patients at time of prior CT examination planned with traditional scout imaging (“prior”) and at time of recent CT examination planned with 3D Landmark and ALD (“new”).

Article Snippet: 61 consecutive patients with prior CT examination of the same body region that underwent clinical CT planned using 3D Landmark Scan and ALD (Canon Medical Aquilion ONE Insight Edition; Otawara, Tochigi, Japan) at a single center over a 3-month period from 7/17/23–10/16/23 were included in this study.

Techniques: Imaging, Standard Deviation

Journal: European Journal of Radiology Open

Article Title: Reduced dose helical CT scout imaging on next generation wide volume CT system decreases scan length and overall radiation exposure

doi: 10.1016/j.ejro.2024.100578

Figure Lengend Snippet: Scan lengths and radiation doses for 104 CT examinations planned using traditional scout imaging (“prior”) and planned using 3D Landmark and ALD (“new”). Radiation doses presented as dose length product (DLP), effective dose (in milisieverts), and CT dose index (CTDI). Total DLP and CTDI reflect the sum of the scout imaging dose and acquisition dose. Scan lengths, DLP and corresponding effective doses of diagnostic acquisitions are further broken into subgroup by body region. Effective radiation dose in millisieverts (mSv) was calculated from the DLP using a k factor of 0.014 for chest, 0.015 for abdomen, and 0.0145 for chest/abdomen/pelvis [13] . Data represented as median and interquartile range. n = 50 for chest CT scan length, n = 16 for abdomen scan length, n = 38 for chest/abdomen/pelvis scan length, and n = 104 for scout scan length. n = 49 for chest radiation doses, n = 15 for abdomen radiation doses, n = 36 for chest/abdomen/pelvis radiation doses, and n = 100 for scout radiation doses.

Article Snippet: 61 consecutive patients with prior CT examination of the same body region that underwent clinical CT planned using 3D Landmark Scan and ALD (Canon Medical Aquilion ONE Insight Edition; Otawara, Tochigi, Japan) at a single center over a 3-month period from 7/17/23–10/16/23 were included in this study.

Techniques: Imaging, Diagnostic Assay, Computed Tomography

Journal: European Journal of Radiology Open

Article Title: Reduced dose helical CT scout imaging on next generation wide volume CT system decreases scan length and overall radiation exposure

doi: 10.1016/j.ejro.2024.100578

Figure Lengend Snippet: Dose-length product (DLP) (mGy·cm) for (a) chest, (b) abdomen, and (c) chest/abdomen/pelvis CT exams planned using 3D Landmark Scan and Anatomic Landmark Detection and performed on next generation CT (“new DLP”) versus those planned using traditional scout methods (“prior DLP”). (a) Median DLP for chest CT was reduced by 11.9 %, from 182.1 (IQR 146.1–283.7) to 160.4 (IQR 133.8–223.6) mGy·cm, p = 0.007, n = 49. (b). Median DLP for abdomen CT was reduced by 47.3 % from 236.8 (IQR 178.1–284.60) to 124.9 (96.8–166.8) mGy·cm, p = 0.004, n = 15. (c) Median DLP for chest/abdomen/pelvis CT was reduced by 28.6 %, from 531.6 (IQR 465.4–689.2) to 379.7 (IQR 319.0–531.8) mGy·cm, respectively, p = 0.001, n = 36.

Article Snippet: 61 consecutive patients with prior CT examination of the same body region that underwent clinical CT planned using 3D Landmark Scan and ALD (Canon Medical Aquilion ONE Insight Edition; Otawara, Tochigi, Japan) at a single center over a 3-month period from 7/17/23–10/16/23 were included in this study.

Techniques:

Journal: European Journal of Radiology Open

Article Title: Reduced dose helical CT scout imaging on next generation wide volume CT system decreases scan length and overall radiation exposure

doi: 10.1016/j.ejro.2024.100578

Figure Lengend Snippet: Scan length (mm) for (a) chest, (b), abdomen, and (c) chest/abdomen/pelvis CT exams planned using 3D Landmark Scan and Anatomic Landmark Detection versus those planned using traditional scout images. (a) Median scan length 317.3 (IQR 302.3–338)mm compared to 330 (IQR 325.8–360)mm, respectively (p < 0.001, n = 50). Median reduction of 12.7 mm represented by the black dashed line. (b) Median scan length 235.5 (IQR 209.5–246)mm compared to 250 (IQR 240.1–260)mm respectively(p = 0.003, n = 16). Median reduction of 14.5 mm represented by the black line. (c) Median scan length 635 (IQR 625–667.3)mm compared to 660 (IQR 641.3–687.3)mm respectively (p < 0.001, n = 38). Median reduction of 25.0 mm represented by the black line.

Article Snippet: 61 consecutive patients with prior CT examination of the same body region that underwent clinical CT planned using 3D Landmark Scan and ALD (Canon Medical Aquilion ONE Insight Edition; Otawara, Tochigi, Japan) at a single center over a 3-month period from 7/17/23–10/16/23 were included in this study.

Techniques: