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Oxford Instruments
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Autodesk Inc
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Autodesk Inc
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Bruker Corporation
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DYNEX tech
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Quidel
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Bayer HealthCare Pharmaceuticals Inc
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Image Search Results
Journal: Glia
Article Title: A novel myelin protein zero transgenic zebrafish designed for rapid readout of in vivo myelination.
doi: 10.1002/glia.23559
Figure Lengend Snippet: Tg(mpz:mEGFP) larvae were incubated in embryo medium with vehicle, 10nM GC1, 10μM WAY200070, 20μM Rapamycin, or 1.5μM AG1478 in 1% DMSO during critical periods of oligodendrocyte lineage progression including initiation of myelination (2–4dpf, A-left panels) or active myelination (3–6dpf, A-right panels). Larvae were anesthetized with tricaine, mounted in low-melt agarose and live imaged using confocal microscopy (A). (B-C) Representative max projections of 3D reconstructions using Imaris image processing software were used to quantify total changes in fluorescence intensity (relative fluorescence units [RFUs]) and compared to vehicle treated controls using equivalent regions of the lateral spinal cord. (D-E) qPCR analysis of mpz, mbp and 36K mRNAs following drug treatments in age-matched larvae was consistent with the quantified changes in EGFP intensity measurements (RFUs). (scale bar = 50μM; statistical analysis: 1-way Anova, p<0.05* <0.01** <0.001*** <0.0001****)
Article Snippet: Larvae were anesthetized with tricaine, mounted in low-melt agarose and live imaged using confocal microscopy (A). (B-C) Representative max projections of
Techniques: Incubation, Confocal Microscopy, Software, Fluorescence
Journal: Glia
Article Title: A novel myelin protein zero transgenic zebrafish designed for rapid readout of in vivo myelination.
doi: 10.1002/glia.23559
Figure Lengend Snippet: Average number of dorsal oligodendrocyte lineage cells (olig2+/sox10+) was quantified from 3D reconstructions of live images of the lateral spinal cord of Tg(olig2:EGFP; sox10:T-RFP) larvae (A) or myelinating oligodendrocytes (olig2+/mbp+) from Tg(olig2:EGFP; mbp1a:T-RFP) larvae (B) following 10nM GC1 or 20μM rapamycin treatment (2–4dpf). (C-E) Representative images of individual scatter labeled oligodendrocytes and quantitative analysis of internode length (F), number of internodes (G) and total internode length generated per cell (H) following 10nM GC1 or 20μM rapamycin treatment (2–4dpf). (scale bar =25μM, statistical analysis: 1-way Anova, p<0.05* <0.01** <0.001*** <0.0001****)
Article Snippet: Larvae were anesthetized with tricaine, mounted in low-melt agarose and live imaged using confocal microscopy (A). (B-C) Representative max projections of
Techniques: Labeling, Generated