3d max 2010 Search Results


99
Oxford Instruments 3d reconstructions
Tg(mpz:mEGFP) larvae were incubated in embryo medium with vehicle, 10nM GC1, 10μM WAY200070, 20μM Rapamycin, or 1.5μM AG1478 in 1% DMSO during critical periods of oligodendrocyte lineage progression including initiation of myelination (2–4dpf, A-left panels) or active myelination (3–6dpf, A-right panels). Larvae were anesthetized with tricaine, mounted in low-melt agarose and live imaged <t>using</t> <t>confocal</t> microscopy (A). (B-C) Representative max projections of <t>3D</t> reconstructions using Imaris image processing software were used to quantify total changes in fluorescence intensity (relative fluorescence units [RFUs]) and compared to vehicle treated controls using equivalent regions of the lateral spinal cord. (D-E) qPCR analysis of mpz, mbp and 36K mRNAs following drug treatments in age-matched larvae was consistent with the quantified changes in EGFP intensity measurements (RFUs). (scale bar = 50μM; statistical analysis: 1-way Anova, p<0.05* <0.01** <0.001*** <0.0001****)
3d Reconstructions, supplied by Oxford Instruments, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 99 stars, based on 1 article reviews
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86
Autodesk Inc 3d max software 2010
Tg(mpz:mEGFP) larvae were incubated in embryo medium with vehicle, 10nM GC1, 10μM WAY200070, 20μM Rapamycin, or 1.5μM AG1478 in 1% DMSO during critical periods of oligodendrocyte lineage progression including initiation of myelination (2–4dpf, A-left panels) or active myelination (3–6dpf, A-right panels). Larvae were anesthetized with tricaine, mounted in low-melt agarose and live imaged <t>using</t> <t>confocal</t> microscopy (A). (B-C) Representative max projections of <t>3D</t> reconstructions using Imaris image processing software were used to quantify total changes in fluorescence intensity (relative fluorescence units [RFUs]) and compared to vehicle treated controls using equivalent regions of the lateral spinal cord. (D-E) qPCR analysis of mpz, mbp and 36K mRNAs following drug treatments in age-matched larvae was consistent with the quantified changes in EGFP intensity measurements (RFUs). (scale bar = 50μM; statistical analysis: 1-way Anova, p<0.05* <0.01** <0.001*** <0.0001****)
3d Max Software 2010, supplied by Autodesk Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/3d max software 2010/product/Autodesk Inc
Average 86 stars, based on 1 article reviews
3d max software 2010 - by Bioz Stars, 2026-05
86/100 stars
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86
Autodesk Inc max design 2010 software
Tg(mpz:mEGFP) larvae were incubated in embryo medium with vehicle, 10nM GC1, 10μM WAY200070, 20μM Rapamycin, or 1.5μM AG1478 in 1% DMSO during critical periods of oligodendrocyte lineage progression including initiation of myelination (2–4dpf, A-left panels) or active myelination (3–6dpf, A-right panels). Larvae were anesthetized with tricaine, mounted in low-melt agarose and live imaged <t>using</t> <t>confocal</t> microscopy (A). (B-C) Representative max projections of <t>3D</t> reconstructions using Imaris image processing software were used to quantify total changes in fluorescence intensity (relative fluorescence units [RFUs]) and compared to vehicle treated controls using equivalent regions of the lateral spinal cord. (D-E) qPCR analysis of mpz, mbp and 36K mRNAs following drug treatments in age-matched larvae was consistent with the quantified changes in EGFP intensity measurements (RFUs). (scale bar = 50μM; statistical analysis: 1-way Anova, p<0.05* <0.01** <0.001*** <0.0001****)
Max Design 2010 Software, supplied by Autodesk Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/max design 2010 software/product/Autodesk Inc
Average 86 stars, based on 1 article reviews
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86/100 stars
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96
Bruker Corporation topspin 4 1 4 bruker
Tg(mpz:mEGFP) larvae were incubated in embryo medium with vehicle, 10nM GC1, 10μM WAY200070, 20μM Rapamycin, or 1.5μM AG1478 in 1% DMSO during critical periods of oligodendrocyte lineage progression including initiation of myelination (2–4dpf, A-left panels) or active myelination (3–6dpf, A-right panels). Larvae were anesthetized with tricaine, mounted in low-melt agarose and live imaged <t>using</t> <t>confocal</t> microscopy (A). (B-C) Representative max projections of <t>3D</t> reconstructions using Imaris image processing software were used to quantify total changes in fluorescence intensity (relative fluorescence units [RFUs]) and compared to vehicle treated controls using equivalent regions of the lateral spinal cord. (D-E) qPCR analysis of mpz, mbp and 36K mRNAs following drug treatments in age-matched larvae was consistent with the quantified changes in EGFP intensity measurements (RFUs). (scale bar = 50μM; statistical analysis: 1-way Anova, p<0.05* <0.01** <0.001*** <0.0001****)
Topspin 4 1 4 Bruker, supplied by Bruker Corporation, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 96 stars, based on 1 article reviews
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90
DYNEX tech agility® system
Tg(mpz:mEGFP) larvae were incubated in embryo medium with vehicle, 10nM GC1, 10μM WAY200070, 20μM Rapamycin, or 1.5μM AG1478 in 1% DMSO during critical periods of oligodendrocyte lineage progression including initiation of myelination (2–4dpf, A-left panels) or active myelination (3–6dpf, A-right panels). Larvae were anesthetized with tricaine, mounted in low-melt agarose and live imaged <t>using</t> <t>confocal</t> microscopy (A). (B-C) Representative max projections of <t>3D</t> reconstructions using Imaris image processing software were used to quantify total changes in fluorescence intensity (relative fluorescence units [RFUs]) and compared to vehicle treated controls using equivalent regions of the lateral spinal cord. (D-E) qPCR analysis of mpz, mbp and 36K mRNAs following drug treatments in age-matched larvae was consistent with the quantified changes in EGFP intensity measurements (RFUs). (scale bar = 50μM; statistical analysis: 1-way Anova, p<0.05* <0.01** <0.001*** <0.0001****)
Agility® System, supplied by DYNEX tech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
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90
Quidel vitros® ecl system
Tg(mpz:mEGFP) larvae were incubated in embryo medium with vehicle, 10nM GC1, 10μM WAY200070, 20μM Rapamycin, or 1.5μM AG1478 in 1% DMSO during critical periods of oligodendrocyte lineage progression including initiation of myelination (2–4dpf, A-left panels) or active myelination (3–6dpf, A-right panels). Larvae were anesthetized with tricaine, mounted in low-melt agarose and live imaged <t>using</t> <t>confocal</t> microscopy (A). (B-C) Representative max projections of <t>3D</t> reconstructions using Imaris image processing software were used to quantify total changes in fluorescence intensity (relative fluorescence units [RFUs]) and compared to vehicle treated controls using equivalent regions of the lateral spinal cord. (D-E) qPCR analysis of mpz, mbp and 36K mRNAs following drug treatments in age-matched larvae was consistent with the quantified changes in EGFP intensity measurements (RFUs). (scale bar = 50μM; statistical analysis: 1-way Anova, p<0.05* <0.01** <0.001*** <0.0001****)
Vitros® Ecl System, supplied by Quidel, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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90
Bayer HealthCare Pharmaceuticals Inc enrofloxacin (baytril)
Tg(mpz:mEGFP) larvae were incubated in embryo medium with vehicle, 10nM GC1, 10μM WAY200070, 20μM Rapamycin, or 1.5μM AG1478 in 1% DMSO during critical periods of oligodendrocyte lineage progression including initiation of myelination (2–4dpf, A-left panels) or active myelination (3–6dpf, A-right panels). Larvae were anesthetized with tricaine, mounted in low-melt agarose and live imaged <t>using</t> <t>confocal</t> microscopy (A). (B-C) Representative max projections of <t>3D</t> reconstructions using Imaris image processing software were used to quantify total changes in fluorescence intensity (relative fluorescence units [RFUs]) and compared to vehicle treated controls using equivalent regions of the lateral spinal cord. (D-E) qPCR analysis of mpz, mbp and 36K mRNAs following drug treatments in age-matched larvae was consistent with the quantified changes in EGFP intensity measurements (RFUs). (scale bar = 50μM; statistical analysis: 1-way Anova, p<0.05* <0.01** <0.001*** <0.0001****)
Enrofloxacin (Baytril), supplied by Bayer HealthCare Pharmaceuticals Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/enrofloxacin (baytril)/product/Bayer HealthCare Pharmaceuticals Inc
Average 90 stars, based on 1 article reviews
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90
AstraZeneca ltd xylocaine liquid (lidocaine 1% + epinephrine 1:100,000 solution)
Tg(mpz:mEGFP) larvae were incubated in embryo medium with vehicle, 10nM GC1, 10μM WAY200070, 20μM Rapamycin, or 1.5μM AG1478 in 1% DMSO during critical periods of oligodendrocyte lineage progression including initiation of myelination (2–4dpf, A-left panels) or active myelination (3–6dpf, A-right panels). Larvae were anesthetized with tricaine, mounted in low-melt agarose and live imaged <t>using</t> <t>confocal</t> microscopy (A). (B-C) Representative max projections of <t>3D</t> reconstructions using Imaris image processing software were used to quantify total changes in fluorescence intensity (relative fluorescence units [RFUs]) and compared to vehicle treated controls using equivalent regions of the lateral spinal cord. (D-E) qPCR analysis of mpz, mbp and 36K mRNAs following drug treatments in age-matched larvae was consistent with the quantified changes in EGFP intensity measurements (RFUs). (scale bar = 50μM; statistical analysis: 1-way Anova, p<0.05* <0.01** <0.001*** <0.0001****)
Xylocaine Liquid (Lidocaine 1% + Epinephrine 1:100,000 Solution), supplied by AstraZeneca ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
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Image Search Results


Tg(mpz:mEGFP) larvae were incubated in embryo medium with vehicle, 10nM GC1, 10μM WAY200070, 20μM Rapamycin, or 1.5μM AG1478 in 1% DMSO during critical periods of oligodendrocyte lineage progression including initiation of myelination (2–4dpf, A-left panels) or active myelination (3–6dpf, A-right panels). Larvae were anesthetized with tricaine, mounted in low-melt agarose and live imaged using confocal microscopy (A). (B-C) Representative max projections of 3D reconstructions using Imaris image processing software were used to quantify total changes in fluorescence intensity (relative fluorescence units [RFUs]) and compared to vehicle treated controls using equivalent regions of the lateral spinal cord. (D-E) qPCR analysis of mpz, mbp and 36K mRNAs following drug treatments in age-matched larvae was consistent with the quantified changes in EGFP intensity measurements (RFUs). (scale bar = 50μM; statistical analysis: 1-way Anova, p<0.05* <0.01** <0.001*** <0.0001****)

Journal: Glia

Article Title: A novel myelin protein zero transgenic zebrafish designed for rapid readout of in vivo myelination.

doi: 10.1002/glia.23559

Figure Lengend Snippet: Tg(mpz:mEGFP) larvae were incubated in embryo medium with vehicle, 10nM GC1, 10μM WAY200070, 20μM Rapamycin, or 1.5μM AG1478 in 1% DMSO during critical periods of oligodendrocyte lineage progression including initiation of myelination (2–4dpf, A-left panels) or active myelination (3–6dpf, A-right panels). Larvae were anesthetized with tricaine, mounted in low-melt agarose and live imaged using confocal microscopy (A). (B-C) Representative max projections of 3D reconstructions using Imaris image processing software were used to quantify total changes in fluorescence intensity (relative fluorescence units [RFUs]) and compared to vehicle treated controls using equivalent regions of the lateral spinal cord. (D-E) qPCR analysis of mpz, mbp and 36K mRNAs following drug treatments in age-matched larvae was consistent with the quantified changes in EGFP intensity measurements (RFUs). (scale bar = 50μM; statistical analysis: 1-way Anova, p<0.05* <0.01** <0.001*** <0.0001****)

Article Snippet: Larvae were anesthetized with tricaine, mounted in low-melt agarose and live imaged using confocal microscopy (A). (B-C) Representative max projections of 3D reconstructions using Imaris image processing software were used to quantify total changes in fluorescence intensity (relative fluorescence units [RFUs]) and compared to vehicle treated controls using equivalent regions of the lateral spinal cord. (D-E) qPCR analysis of mpz, mbp and 36K mRNAs following drug treatments in age-matched larvae was consistent with the quantified changes in EGFP intensity measurements (RFUs). (scale bar = 50μM; statistical analysis: 1-way Anova, p<0.05* <0.01** <0.001*** <0.0001****) qPCR analysis for common myelin mRNAs showed relatively comparable responses to the drugs ( ). mpz , mbp and 36K transcripts, which are the most well characterized myelin-specific transcripts in zebrafish ( Q. Bai, M. Sun, D. B. Stolz, & E. A. Burton, 2011b ; C. E. Buckley et al., 2010 ; Morris et al., 2004 ) were quantified, and the changes in EGFP intensity generally mirrored changes in mpz mRNA expression as assayed by qPCR (Relative changes [RQ] for GC1: 2.29 fold increase, p<0.0166; Way200070: 1.75 fold increase, p<0.0079; rapamycin: 0.40 fold vs DMSO, p<0.0182 and Ag1478: 0.77 fold decrease, p<0.402; ).

Techniques: Incubation, Confocal Microscopy, Software, Fluorescence

Average number of dorsal oligodendrocyte lineage cells (olig2+/sox10+) was quantified from 3D reconstructions of live images of the lateral spinal cord of Tg(olig2:EGFP; sox10:T-RFP) larvae (A) or myelinating oligodendrocytes (olig2+/mbp+) from Tg(olig2:EGFP; mbp1a:T-RFP) larvae (B) following 10nM GC1 or 20μM rapamycin treatment (2–4dpf). (C-E) Representative images of individual scatter labeled oligodendrocytes and quantitative analysis of internode length (F), number of internodes (G) and total internode length generated per cell (H) following 10nM GC1 or 20μM rapamycin treatment (2–4dpf). (scale bar =25μM, statistical analysis: 1-way Anova, p<0.05* <0.01** <0.001*** <0.0001****)

Journal: Glia

Article Title: A novel myelin protein zero transgenic zebrafish designed for rapid readout of in vivo myelination.

doi: 10.1002/glia.23559

Figure Lengend Snippet: Average number of dorsal oligodendrocyte lineage cells (olig2+/sox10+) was quantified from 3D reconstructions of live images of the lateral spinal cord of Tg(olig2:EGFP; sox10:T-RFP) larvae (A) or myelinating oligodendrocytes (olig2+/mbp+) from Tg(olig2:EGFP; mbp1a:T-RFP) larvae (B) following 10nM GC1 or 20μM rapamycin treatment (2–4dpf). (C-E) Representative images of individual scatter labeled oligodendrocytes and quantitative analysis of internode length (F), number of internodes (G) and total internode length generated per cell (H) following 10nM GC1 or 20μM rapamycin treatment (2–4dpf). (scale bar =25μM, statistical analysis: 1-way Anova, p<0.05* <0.01** <0.001*** <0.0001****)

Article Snippet: Larvae were anesthetized with tricaine, mounted in low-melt agarose and live imaged using confocal microscopy (A). (B-C) Representative max projections of 3D reconstructions using Imaris image processing software were used to quantify total changes in fluorescence intensity (relative fluorescence units [RFUs]) and compared to vehicle treated controls using equivalent regions of the lateral spinal cord. (D-E) qPCR analysis of mpz, mbp and 36K mRNAs following drug treatments in age-matched larvae was consistent with the quantified changes in EGFP intensity measurements (RFUs). (scale bar = 50μM; statistical analysis: 1-way Anova, p<0.05* <0.01** <0.001*** <0.0001****) qPCR analysis for common myelin mRNAs showed relatively comparable responses to the drugs ( ). mpz , mbp and 36K transcripts, which are the most well characterized myelin-specific transcripts in zebrafish ( Q. Bai, M. Sun, D. B. Stolz, & E. A. Burton, 2011b ; C. E. Buckley et al., 2010 ; Morris et al., 2004 ) were quantified, and the changes in EGFP intensity generally mirrored changes in mpz mRNA expression as assayed by qPCR (Relative changes [RQ] for GC1: 2.29 fold increase, p<0.0166; Way200070: 1.75 fold increase, p<0.0079; rapamycin: 0.40 fold vs DMSO, p<0.0182 and Ag1478: 0.77 fold decrease, p<0.402; ).

Techniques: Labeling, Generated