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Image Search Results
Journal: Nucleic Acids Research
Article Title: High-throughput alternative splicing quantification by primer extension and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry
doi: 10.1093/nar/gni098
Figure Lengend Snippet: Alternative splicing isoform analysis via primer extension. All classifications of splicing can be analyzed including exon insertion ( a ), exon deletion ( b ), 3′ splicing acceptor ( c ), 5′ splicing donor ( d ) and mutually exclusive exons ( e ). Exon lengths are not to scale. Primer extension assays of respective genes were assayed on a MALDI-TOF MS. In the mass spectra, extension primer refers to unextended primers, while the subsequent peaks indicate the primer with an extension of the base(s) specified.
Article Snippet: ASV analysis was performed using the MassARRAY system (Sequenom) by dispensing ≈10 nl of final product onto a
Techniques: