33-mer peptide Search Results


27-mer peptides  (GenScript corporation)
90
GenScript corporation 27-mer peptides
27 Mer Peptides, supplied by GenScript corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/33-mer+peptide/33+mer+peptide/10__1128_slash_jvi__01373___13-39-56-87
Average 90 stars, based on 1 article reviews
27-mer peptides - by Bioz Stars, 2026-10
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native 33-mer peptide (lqlqpf (pqpqlpy) 3 pqpqpf  (GL Biochem)
90
GL Biochem native 33-mer peptide (lqlqpf (pqpqlpy) 3 pqpqpf
Native 33 Mer Peptide (Lqlqpf (Pqpqlpy) 3 Pqpqpf, supplied by GL Biochem, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/33-mer+peptide/native+33+mer+peptide/pmc05579586-19-2-11
Average 90 stars, based on 1 article reviews
native 33-mer peptide (lqlqpf (pqpqlpy) 3 pqpqpf - by Bioz Stars, 2026-10
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33-mer peptide  (Biomedal Inc)
90
Biomedal Inc 33-mer peptide
33 Mer Peptide, supplied by Biomedal Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/33-mer+peptide/33+mer+peptide/pm24963630-66-18-23
Average 90 stars, based on 1 article reviews
33-mer peptide - by Bioz Stars, 2026-10
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deamidated 33-mer (lqlqpf (pqp e lpy) 3 pqpqpf)  (MultiSynTech gmbh)
90
MultiSynTech gmbh deamidated 33-mer (lqlqpf (pqp e lpy) 3 pqpqpf)
Transamidation pattern of DQ2.5-glia-α1a, DQ2.5-glia-α2, and <t>33-mer</t> peptide after modification using mTG and three different acyl-acceptor molecules. (A) Effect of pH on 33-mer reaction induced by mTG in the presence of Lys. (B) Effect of pH on 33-mer reaction induced by mTG in the presence of HA. (C) Effect of GEE concentration on 33-mer transamidation induced by mTG at pH 6.0. Molar ratio between the 33-mer peptide and GEE is 1:0.84, 1:3.32, 1:13.16, 1:52.63, 1:210.53 as shown. (D) Transamidation pattern of 33-mer by mTG. As compared with native peptide, 33-mer cross-linked to Lys, GEE, and HA resulted in a shift of 129 × 3 Da, 86 × 3 Da and 16 × 3 Da, corresponding to the addition of three Lys, GEE, and HA groups, respectively. (E) Transamidation pattern of DQ2.5-glia-α1a by mTG. DQ2.5-glia-α1a presented 129 Da, 86 Da, and 16 Da shift responding to the addition of a single Lys, GEE, and HA group. (F) Transamidation pattern of DQ2.5-glia-α2 by mTG. DQ2.5-glia-α2 presented 129 Da, 86 Da, and 16 Da shift responding to the addition of a single Lys, GEE, and HA group. Monoisotopic mass are shown throughout.
Deamidated 33 Mer (Lqlqpf (Pqp E Lpy) 3 Pqpqpf), supplied by MultiSynTech gmbh, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/33-mer+peptide/deamidated+33+mer++lqlqpf++pqpelpy+3+pqpqpf++peptides/pmc05579586-18-22-42
Average 90 stars, based on 1 article reviews
deamidated 33-mer (lqlqpf (pqp e lpy) 3 pqpqpf) - by Bioz Stars, 2026-10
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gliadin-derived immunogenic 33-mer peptide  (21st Century Biochemicals)
90
21st Century Biochemicals gliadin-derived immunogenic 33-mer peptide
Transamidation pattern of DQ2.5-glia-α1a, DQ2.5-glia-α2, and <t>33-mer</t> peptide after modification using mTG and three different acyl-acceptor molecules. (A) Effect of pH on 33-mer reaction induced by mTG in the presence of Lys. (B) Effect of pH on 33-mer reaction induced by mTG in the presence of HA. (C) Effect of GEE concentration on 33-mer transamidation induced by mTG at pH 6.0. Molar ratio between the 33-mer peptide and GEE is 1:0.84, 1:3.32, 1:13.16, 1:52.63, 1:210.53 as shown. (D) Transamidation pattern of 33-mer by mTG. As compared with native peptide, 33-mer cross-linked to Lys, GEE, and HA resulted in a shift of 129 × 3 Da, 86 × 3 Da and 16 × 3 Da, corresponding to the addition of three Lys, GEE, and HA groups, respectively. (E) Transamidation pattern of DQ2.5-glia-α1a by mTG. DQ2.5-glia-α1a presented 129 Da, 86 Da, and 16 Da shift responding to the addition of a single Lys, GEE, and HA group. (F) Transamidation pattern of DQ2.5-glia-α2 by mTG. DQ2.5-glia-α2 presented 129 Da, 86 Da, and 16 Da shift responding to the addition of a single Lys, GEE, and HA group. Monoisotopic mass are shown throughout.
Gliadin Derived Immunogenic 33 Mer Peptide, supplied by 21st Century Biochemicals, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/33-mer+peptide/33+mer+peptide/pmc05335528-218-1-21
Average 90 stars, based on 1 article reviews
gliadin-derived immunogenic 33-mer peptide - by Bioz Stars, 2026-10
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biotinylated 33-mer peptide  (Biomedal Inc)
90
Biomedal Inc biotinylated 33-mer peptide
Transamidation pattern of DQ2.5-glia-α1a, DQ2.5-glia-α2, and <t>33-mer</t> peptide after modification using mTG and three different acyl-acceptor molecules. (A) Effect of pH on 33-mer reaction induced by mTG in the presence of Lys. (B) Effect of pH on 33-mer reaction induced by mTG in the presence of HA. (C) Effect of GEE concentration on 33-mer transamidation induced by mTG at pH 6.0. Molar ratio between the 33-mer peptide and GEE is 1:0.84, 1:3.32, 1:13.16, 1:52.63, 1:210.53 as shown. (D) Transamidation pattern of 33-mer by mTG. As compared with native peptide, 33-mer cross-linked to Lys, GEE, and HA resulted in a shift of 129 × 3 Da, 86 × 3 Da and 16 × 3 Da, corresponding to the addition of three Lys, GEE, and HA groups, respectively. (E) Transamidation pattern of DQ2.5-glia-α1a by mTG. DQ2.5-glia-α1a presented 129 Da, 86 Da, and 16 Da shift responding to the addition of a single Lys, GEE, and HA group. (F) Transamidation pattern of DQ2.5-glia-α2 by mTG. DQ2.5-glia-α2 presented 129 Da, 86 Da, and 16 Da shift responding to the addition of a single Lys, GEE, and HA group. Monoisotopic mass are shown throughout.
Biotinylated 33 Mer Peptide, supplied by Biomedal Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/33-mer+peptide/biotinylated+33+mer+peptide/10__1016_slash_j__snb__2016__10__112-67-0-6
Average 90 stars, based on 1 article reviews
biotinylated 33-mer peptide - by Bioz Stars, 2026-10
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peptide lqlqpfpqpqlpypqpqlpypqpqplpypqpqpf (33-mer)  (GenScript corporation)
90
GenScript corporation peptide lqlqpfpqpqlpypqpqlpypqpqplpypqpqpf (33-mer)
Transamidation pattern of DQ2.5-glia-α1a, DQ2.5-glia-α2, and <t>33-mer</t> peptide after modification using mTG and three different acyl-acceptor molecules. (A) Effect of pH on 33-mer reaction induced by mTG in the presence of Lys. (B) Effect of pH on 33-mer reaction induced by mTG in the presence of HA. (C) Effect of GEE concentration on 33-mer transamidation induced by mTG at pH 6.0. Molar ratio between the 33-mer peptide and GEE is 1:0.84, 1:3.32, 1:13.16, 1:52.63, 1:210.53 as shown. (D) Transamidation pattern of 33-mer by mTG. As compared with native peptide, 33-mer cross-linked to Lys, GEE, and HA resulted in a shift of 129 × 3 Da, 86 × 3 Da and 16 × 3 Da, corresponding to the addition of three Lys, GEE, and HA groups, respectively. (E) Transamidation pattern of DQ2.5-glia-α1a by mTG. DQ2.5-glia-α1a presented 129 Da, 86 Da, and 16 Da shift responding to the addition of a single Lys, GEE, and HA group. (F) Transamidation pattern of DQ2.5-glia-α2 by mTG. DQ2.5-glia-α2 presented 129 Da, 86 Da, and 16 Da shift responding to the addition of a single Lys, GEE, and HA group. Monoisotopic mass are shown throughout.
Peptide Lqlqpfpqpqlpypqpqlpypqpqplpypqpqpf (33 Mer), supplied by GenScript corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/33-mer+peptide/peptide+lqlqpfpqpqlpypqpqlpypqpqplpypqpqpf++33+mer+/pmc05361186-202-1-32
Average 90 stars, based on 1 article reviews
peptide lqlqpfpqpqlpypqpqlpypqpqplpypqpqpf (33-mer) - by Bioz Stars, 2026-10
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33-mer gliadin peptide (lqlqpfpqpqlpypqpqlpypqpqlpypqpqpf)  (GL Biochem)
90
GL Biochem 33-mer gliadin peptide (lqlqpfpqpqlpypqpqlpypqpqlpypqpqpf)
Transamidation pattern of DQ2.5-glia-α1a, DQ2.5-glia-α2, and <t>33-mer</t> peptide after modification using mTG and three different acyl-acceptor molecules. (A) Effect of pH on 33-mer reaction induced by mTG in the presence of Lys. (B) Effect of pH on 33-mer reaction induced by mTG in the presence of HA. (C) Effect of GEE concentration on 33-mer transamidation induced by mTG at pH 6.0. Molar ratio between the 33-mer peptide and GEE is 1:0.84, 1:3.32, 1:13.16, 1:52.63, 1:210.53 as shown. (D) Transamidation pattern of 33-mer by mTG. As compared with native peptide, 33-mer cross-linked to Lys, GEE, and HA resulted in a shift of 129 × 3 Da, 86 × 3 Da and 16 × 3 Da, corresponding to the addition of three Lys, GEE, and HA groups, respectively. (E) Transamidation pattern of DQ2.5-glia-α1a by mTG. DQ2.5-glia-α1a presented 129 Da, 86 Da, and 16 Da shift responding to the addition of a single Lys, GEE, and HA group. (F) Transamidation pattern of DQ2.5-glia-α2 by mTG. DQ2.5-glia-α2 presented 129 Da, 86 Da, and 16 Da shift responding to the addition of a single Lys, GEE, and HA group. Monoisotopic mass are shown throughout.
33 Mer Gliadin Peptide (Lqlqpfpqpqlpypqpqlpypqpqlpypqpqpf), supplied by GL Biochem, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/33-mer+peptide/33+mer+gliadin+peptide++lqlqpfpqpqlpypqpqlpypqpqlpypqpqpf+/pmc08392533-90-1-8
Average 90 stars, based on 1 article reviews
33-mer gliadin peptide (lqlqpfpqpqlpypqpqlpypqpqlpypqpqpf) - by Bioz Stars, 2026-10
90/100 stars
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deamidated celiac disease antigen 33-mer α2 peptide (lqlqpfpqpelpypqpelpypqpqlpypqpqpf)  (GenScript corporation)
90
GenScript corporation deamidated celiac disease antigen 33-mer α2 peptide (lqlqpfpqpelpypqpelpypqpqlpypqpqpf)
Transamidation pattern of DQ2.5-glia-α1a, DQ2.5-glia-α2, and <t>33-mer</t> peptide after modification using mTG and three different acyl-acceptor molecules. (A) Effect of pH on 33-mer reaction induced by mTG in the presence of Lys. (B) Effect of pH on 33-mer reaction induced by mTG in the presence of HA. (C) Effect of GEE concentration on 33-mer transamidation induced by mTG at pH 6.0. Molar ratio between the 33-mer peptide and GEE is 1:0.84, 1:3.32, 1:13.16, 1:52.63, 1:210.53 as shown. (D) Transamidation pattern of 33-mer by mTG. As compared with native peptide, 33-mer cross-linked to Lys, GEE, and HA resulted in a shift of 129 × 3 Da, 86 × 3 Da and 16 × 3 Da, corresponding to the addition of three Lys, GEE, and HA groups, respectively. (E) Transamidation pattern of DQ2.5-glia-α1a by mTG. DQ2.5-glia-α1a presented 129 Da, 86 Da, and 16 Da shift responding to the addition of a single Lys, GEE, and HA group. (F) Transamidation pattern of DQ2.5-glia-α2 by mTG. DQ2.5-glia-α2 presented 129 Da, 86 Da, and 16 Da shift responding to the addition of a single Lys, GEE, and HA group. Monoisotopic mass are shown throughout.
Deamidated Celiac Disease Antigen 33 Mer α2 Peptide (Lqlqpfpqpelpypqpelpypqpqlpypqpqpf), supplied by GenScript corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/33-mer+peptide/deamidated+celiac+disease+antigen+33+mer+%CE%B12+peptide++lqlqpfpqpelpypqpelpypqpqlpypqpqpf+/us11547662-392-25-37
Average 90 stars, based on 1 article reviews
deamidated celiac disease antigen 33-mer α2 peptide (lqlqpfpqpelpypqpelpypqpqlpypqpqpf) - by Bioz Stars, 2026-10
90/100 stars
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synthetic deamidated 33-mer peptide  (EZBiolab Inc)
90
EZBiolab Inc synthetic deamidated 33-mer peptide
Transamidation pattern of DQ2.5-glia-α1a, DQ2.5-glia-α2, and <t>33-mer</t> peptide after modification using mTG and three different acyl-acceptor molecules. (A) Effect of pH on 33-mer reaction induced by mTG in the presence of Lys. (B) Effect of pH on 33-mer reaction induced by mTG in the presence of HA. (C) Effect of GEE concentration on 33-mer transamidation induced by mTG at pH 6.0. Molar ratio between the 33-mer peptide and GEE is 1:0.84, 1:3.32, 1:13.16, 1:52.63, 1:210.53 as shown. (D) Transamidation pattern of 33-mer by mTG. As compared with native peptide, 33-mer cross-linked to Lys, GEE, and HA resulted in a shift of 129 × 3 Da, 86 × 3 Da and 16 × 3 Da, corresponding to the addition of three Lys, GEE, and HA groups, respectively. (E) Transamidation pattern of DQ2.5-glia-α1a by mTG. DQ2.5-glia-α1a presented 129 Da, 86 Da, and 16 Da shift responding to the addition of a single Lys, GEE, and HA group. (F) Transamidation pattern of DQ2.5-glia-α2 by mTG. DQ2.5-glia-α2 presented 129 Da, 86 Da, and 16 Da shift responding to the addition of a single Lys, GEE, and HA group. Monoisotopic mass are shown throughout.
Synthetic Deamidated 33 Mer Peptide, supplied by EZBiolab Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/33-mer+peptide/synthetic+deamidated+33+mer+peptide/pm17309775-59-3-19
Average 90 stars, based on 1 article reviews
synthetic deamidated 33-mer peptide - by Bioz Stars, 2026-10
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synthetic 33-mer peptides (mw = 3911.5 g/mol, purity 95.1%)  (GenScript corporation)
90
GenScript corporation synthetic 33-mer peptides (mw = 3911.5 g/mol, purity 95.1%)
Transamidation pattern of DQ2.5-glia-α1a, DQ2.5-glia-α2, and <t>33-mer</t> peptide after modification using mTG and three different acyl-acceptor molecules. (A) Effect of pH on 33-mer reaction induced by mTG in the presence of Lys. (B) Effect of pH on 33-mer reaction induced by mTG in the presence of HA. (C) Effect of GEE concentration on 33-mer transamidation induced by mTG at pH 6.0. Molar ratio between the 33-mer peptide and GEE is 1:0.84, 1:3.32, 1:13.16, 1:52.63, 1:210.53 as shown. (D) Transamidation pattern of 33-mer by mTG. As compared with native peptide, 33-mer cross-linked to Lys, GEE, and HA resulted in a shift of 129 × 3 Da, 86 × 3 Da and 16 × 3 Da, corresponding to the addition of three Lys, GEE, and HA groups, respectively. (E) Transamidation pattern of DQ2.5-glia-α1a by mTG. DQ2.5-glia-α1a presented 129 Da, 86 Da, and 16 Da shift responding to the addition of a single Lys, GEE, and HA group. (F) Transamidation pattern of DQ2.5-glia-α2 by mTG. DQ2.5-glia-α2 presented 129 Da, 86 Da, and 16 Da shift responding to the addition of a single Lys, GEE, and HA group. Monoisotopic mass are shown throughout.
Synthetic 33 Mer Peptides (Mw = 3911.5 G/Mol, Purity 95.1%), supplied by GenScript corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/33-mer+peptide/synthetic+33+mer+peptides++mw+++3911+5+g+mol++purity+95+1++/pm23870922-64-1-28
Average 90 stars, based on 1 article reviews
synthetic 33-mer peptides (mw = 3911.5 g/mol, purity 95.1%) - by Bioz Stars, 2026-10
90/100 stars
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33-mer peptide  (Wieser GmbH)
90
Wieser GmbH 33-mer peptide
Transamidation pattern of DQ2.5-glia-α1a, DQ2.5-glia-α2, and <t>33-mer</t> peptide after modification using mTG and three different acyl-acceptor molecules. (A) Effect of pH on 33-mer reaction induced by mTG in the presence of Lys. (B) Effect of pH on 33-mer reaction induced by mTG in the presence of HA. (C) Effect of GEE concentration on 33-mer transamidation induced by mTG at pH 6.0. Molar ratio between the 33-mer peptide and GEE is 1:0.84, 1:3.32, 1:13.16, 1:52.63, 1:210.53 as shown. (D) Transamidation pattern of 33-mer by mTG. As compared with native peptide, 33-mer cross-linked to Lys, GEE, and HA resulted in a shift of 129 × 3 Da, 86 × 3 Da and 16 × 3 Da, corresponding to the addition of three Lys, GEE, and HA groups, respectively. (E) Transamidation pattern of DQ2.5-glia-α1a by mTG. DQ2.5-glia-α1a presented 129 Da, 86 Da, and 16 Da shift responding to the addition of a single Lys, GEE, and HA group. (F) Transamidation pattern of DQ2.5-glia-α2 by mTG. DQ2.5-glia-α2 presented 129 Da, 86 Da, and 16 Da shift responding to the addition of a single Lys, GEE, and HA group. Monoisotopic mass are shown throughout.
33 Mer Peptide, supplied by Wieser GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/33-mer+peptide/33+mer+peptide/pm31000227-131-5-18
Average 90 stars, based on 1 article reviews
33-mer peptide - by Bioz Stars, 2026-10
90/100 stars
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Image Search Results


Transamidation pattern of DQ2.5-glia-α1a, DQ2.5-glia-α2, and 33-mer peptide after modification using mTG and three different acyl-acceptor molecules. (A) Effect of pH on 33-mer reaction induced by mTG in the presence of Lys. (B) Effect of pH on 33-mer reaction induced by mTG in the presence of HA. (C) Effect of GEE concentration on 33-mer transamidation induced by mTG at pH 6.0. Molar ratio between the 33-mer peptide and GEE is 1:0.84, 1:3.32, 1:13.16, 1:52.63, 1:210.53 as shown. (D) Transamidation pattern of 33-mer by mTG. As compared with native peptide, 33-mer cross-linked to Lys, GEE, and HA resulted in a shift of 129 × 3 Da, 86 × 3 Da and 16 × 3 Da, corresponding to the addition of three Lys, GEE, and HA groups, respectively. (E) Transamidation pattern of DQ2.5-glia-α1a by mTG. DQ2.5-glia-α1a presented 129 Da, 86 Da, and 16 Da shift responding to the addition of a single Lys, GEE, and HA group. (F) Transamidation pattern of DQ2.5-glia-α2 by mTG. DQ2.5-glia-α2 presented 129 Da, 86 Da, and 16 Da shift responding to the addition of a single Lys, GEE, and HA group. Monoisotopic mass are shown throughout.

Journal: Journal of Agricultural and Food Chemistry

Article Title: Abrogation of Immunogenic Properties of Gliadin Peptides through Transamidation by Microbial Transglutaminase Is Acyl-Acceptor Dependent

doi: 10.1021/acs.jafc.7b02557

Figure Lengend Snippet: Transamidation pattern of DQ2.5-glia-α1a, DQ2.5-glia-α2, and 33-mer peptide after modification using mTG and three different acyl-acceptor molecules. (A) Effect of pH on 33-mer reaction induced by mTG in the presence of Lys. (B) Effect of pH on 33-mer reaction induced by mTG in the presence of HA. (C) Effect of GEE concentration on 33-mer transamidation induced by mTG at pH 6.0. Molar ratio between the 33-mer peptide and GEE is 1:0.84, 1:3.32, 1:13.16, 1:52.63, 1:210.53 as shown. (D) Transamidation pattern of 33-mer by mTG. As compared with native peptide, 33-mer cross-linked to Lys, GEE, and HA resulted in a shift of 129 × 3 Da, 86 × 3 Da and 16 × 3 Da, corresponding to the addition of three Lys, GEE, and HA groups, respectively. (E) Transamidation pattern of DQ2.5-glia-α1a by mTG. DQ2.5-glia-α1a presented 129 Da, 86 Da, and 16 Da shift responding to the addition of a single Lys, GEE, and HA group. (F) Transamidation pattern of DQ2.5-glia-α2 by mTG. DQ2.5-glia-α2 presented 129 Da, 86 Da, and 16 Da shift responding to the addition of a single Lys, GEE, and HA group. Monoisotopic mass are shown throughout.

Article Snippet: DQ2.5-glia-α1a (native form LQPFPQPQLPYPQ and deamidated form LQPFPQP E LPYAA), DQ2.5-glia-α2 (native form PFPQPQLPYPQPQ and deamidated form AAPQP E LPYPQPQ), and deamidated 33-mer (LQLQPF (PQP E LPY) 3 PQPQPF) peptides were synthesized by standard Fmoc chemistry on a multiple peptide synthesizer (Syroll, MultiSynTech GmbH, Witten, Germany).

Techniques: Modification, Concentration Assay

Reactivity pattern of glia-α1and glia-α2-specific-T-cell clones to DQ2.5-glia-α1a, DQ2.5-glia-α2, 33-mer, gliadin, and their mTG-treated forms. (A) Reactivity pattern of glia-α1-specific T-cell clone S2 and L5107 to DQ2.5-glia-α1a and its modified products (5 concentrations tested in each group). (B) Reactivity pattern of glia-α2-specific T-cell clones S16 and D1 to DQ2.5-glia-α2 and its modified products (5 concentrations tested with S16 and only the highest concentration with D1). (C) Reactivity pattern of glia-α1-specific T-cell clones S2, and L5107 to 33-mer and its modified products (5 concentrations tested in each group). (D) Reactivity pattern of glia-α2-specific T-cell clones S16 and D1 to 33-mer and its modified products (5 concentrations tested with S16 and only the highest concentration with D1). (E) Reactivity pattern of glia-α1-specific T-cell clones L6 and L10 to DQ2.5-glia-α1a, 33-mer and their modified products (only the highest concentration tested). (F) Reactivity pattern of glia-α2-specific T-cell clones 101136 and L3-13 to DQ2.5-glia-α2, 33-mer and their modified products (only the highest concentration tested). (G) Reactivity pattern of glia-α1-specific T-cell clones to gliadin protein and its modified products (only the highest concentration tested). (H) Reactivity pattern of glia-α2-specific T-cell clones to gliadin protein and its modified products (only the highest concentration tested). PC, deamidated peptides as positive control; NC, native peptides as negative control. Relative response: CPM of T cell clone caused by peptide/that of caused by deamidated peptide (positive control).

Journal: Journal of Agricultural and Food Chemistry

Article Title: Abrogation of Immunogenic Properties of Gliadin Peptides through Transamidation by Microbial Transglutaminase Is Acyl-Acceptor Dependent

doi: 10.1021/acs.jafc.7b02557

Figure Lengend Snippet: Reactivity pattern of glia-α1and glia-α2-specific-T-cell clones to DQ2.5-glia-α1a, DQ2.5-glia-α2, 33-mer, gliadin, and their mTG-treated forms. (A) Reactivity pattern of glia-α1-specific T-cell clone S2 and L5107 to DQ2.5-glia-α1a and its modified products (5 concentrations tested in each group). (B) Reactivity pattern of glia-α2-specific T-cell clones S16 and D1 to DQ2.5-glia-α2 and its modified products (5 concentrations tested with S16 and only the highest concentration with D1). (C) Reactivity pattern of glia-α1-specific T-cell clones S2, and L5107 to 33-mer and its modified products (5 concentrations tested in each group). (D) Reactivity pattern of glia-α2-specific T-cell clones S16 and D1 to 33-mer and its modified products (5 concentrations tested with S16 and only the highest concentration with D1). (E) Reactivity pattern of glia-α1-specific T-cell clones L6 and L10 to DQ2.5-glia-α1a, 33-mer and their modified products (only the highest concentration tested). (F) Reactivity pattern of glia-α2-specific T-cell clones 101136 and L3-13 to DQ2.5-glia-α2, 33-mer and their modified products (only the highest concentration tested). (G) Reactivity pattern of glia-α1-specific T-cell clones to gliadin protein and its modified products (only the highest concentration tested). (H) Reactivity pattern of glia-α2-specific T-cell clones to gliadin protein and its modified products (only the highest concentration tested). PC, deamidated peptides as positive control; NC, native peptides as negative control. Relative response: CPM of T cell clone caused by peptide/that of caused by deamidated peptide (positive control).

Article Snippet: DQ2.5-glia-α1a (native form LQPFPQPQLPYPQ and deamidated form LQPFPQP E LPYAA), DQ2.5-glia-α2 (native form PFPQPQLPYPQPQ and deamidated form AAPQP E LPYPQPQ), and deamidated 33-mer (LQLQPF (PQP E LPY) 3 PQPQPF) peptides were synthesized by standard Fmoc chemistry on a multiple peptide synthesizer (Syroll, MultiSynTech GmbH, Witten, Germany).

Techniques: Clone Assay, Modification, Concentration Assay, Positive Control, Negative Control

Analysis of DQ2.5-glia-α1a and DQ2.5-glia-α2 after mTG and tTG treatment by MALDI-TOF. (A) Mass spectrum of DQ2.5-glia-α1a (upper panel), DQ2.5-glia-α1a after mTG transamidation with Lys (mTG-Lys, middle panel), and after tTG treatment of Lys transamidated DQ2.5-glia-α1a (tTG-mTG-Lys, lower panel). (B) Mass spectrum of DQ2.5-glia-α2 (upper panel), DQ2.5-glia-α2 after mTG transamidation with Lys (mTG-Lys, middle panel), and after tTG treatment of Lys transamidated DQ2.5-glia-α2 (tTG-mTG-Lys, lower panel). (C) Mass spectrum of 33-mer (upper panel), 33-mer after mTG transamidation with Lys (mTG-Lys, middle panel), and after tTG treatment of Lys transamidated 33-mer (tTG-mTG-Lys, lower panel). The deamidation of Q → E would result in a 1 Da shift. Monoisotopic mass are shown throughout. Arrows indicate the shift in Da after modification.

Journal: Journal of Agricultural and Food Chemistry

Article Title: Abrogation of Immunogenic Properties of Gliadin Peptides through Transamidation by Microbial Transglutaminase Is Acyl-Acceptor Dependent

doi: 10.1021/acs.jafc.7b02557

Figure Lengend Snippet: Analysis of DQ2.5-glia-α1a and DQ2.5-glia-α2 after mTG and tTG treatment by MALDI-TOF. (A) Mass spectrum of DQ2.5-glia-α1a (upper panel), DQ2.5-glia-α1a after mTG transamidation with Lys (mTG-Lys, middle panel), and after tTG treatment of Lys transamidated DQ2.5-glia-α1a (tTG-mTG-Lys, lower panel). (B) Mass spectrum of DQ2.5-glia-α2 (upper panel), DQ2.5-glia-α2 after mTG transamidation with Lys (mTG-Lys, middle panel), and after tTG treatment of Lys transamidated DQ2.5-glia-α2 (tTG-mTG-Lys, lower panel). (C) Mass spectrum of 33-mer (upper panel), 33-mer after mTG transamidation with Lys (mTG-Lys, middle panel), and after tTG treatment of Lys transamidated 33-mer (tTG-mTG-Lys, lower panel). The deamidation of Q → E would result in a 1 Da shift. Monoisotopic mass are shown throughout. Arrows indicate the shift in Da after modification.

Article Snippet: DQ2.5-glia-α1a (native form LQPFPQPQLPYPQ and deamidated form LQPFPQP E LPYAA), DQ2.5-glia-α2 (native form PFPQPQLPYPQPQ and deamidated form AAPQP E LPYPQPQ), and deamidated 33-mer (LQLQPF (PQP E LPY) 3 PQPQPF) peptides were synthesized by standard Fmoc chemistry on a multiple peptide synthesizer (Syroll, MultiSynTech GmbH, Witten, Germany).

Techniques: Modification

tTG can revert mTG-mediated transamidation. (A) Reactivity pattern of glia-α1-specific T-cell clone L5107 to DQ2.5-glia-α1a (left panel), the 33-mer (right panel) before (NC) and after mTG transamidation with Lys (mTG-Lys) and after tTG treatment of mTG-Lys (tTG-mTG-Lys). (B) Reactivity pattern of glia-α1-specific T-cell clones S2 and L6 to DQ2.5-glia-α1a, 33-mer, and their modified products. (C) Reactivity pattern of glia-α2-specific T-cell clone S16 to DQ2.5-glia-α2 (left panel) and the 33-mer (right panel) before (NC) and after mTG transamidation with Lys (mTG-Lys) and after tTG treatment of mTG-Lys (tTG-mTG-Lys). (D) Reactivity pattern of glia-α2-specific T-cell clones D1 and 101136 to DQ2.5-glia-α2, 33-mer, and their modified products. (E) Reactivity pattern of glia-α1-specific T-cell clones to gliadin protein before (NC) and after mTG transamidation with Lys (mTG-Lys) and after tTG treatment of mTG-Lys (tTG-mTG-Lys). Three different concentrations of lysine were used, 10, 40, and 160 mM as indicated. (F) Reactivity pattern of glia-α2-specific T-cell clones to gliadin protein and its modified products.

Journal: Journal of Agricultural and Food Chemistry

Article Title: Abrogation of Immunogenic Properties of Gliadin Peptides through Transamidation by Microbial Transglutaminase Is Acyl-Acceptor Dependent

doi: 10.1021/acs.jafc.7b02557

Figure Lengend Snippet: tTG can revert mTG-mediated transamidation. (A) Reactivity pattern of glia-α1-specific T-cell clone L5107 to DQ2.5-glia-α1a (left panel), the 33-mer (right panel) before (NC) and after mTG transamidation with Lys (mTG-Lys) and after tTG treatment of mTG-Lys (tTG-mTG-Lys). (B) Reactivity pattern of glia-α1-specific T-cell clones S2 and L6 to DQ2.5-glia-α1a, 33-mer, and their modified products. (C) Reactivity pattern of glia-α2-specific T-cell clone S16 to DQ2.5-glia-α2 (left panel) and the 33-mer (right panel) before (NC) and after mTG transamidation with Lys (mTG-Lys) and after tTG treatment of mTG-Lys (tTG-mTG-Lys). (D) Reactivity pattern of glia-α2-specific T-cell clones D1 and 101136 to DQ2.5-glia-α2, 33-mer, and their modified products. (E) Reactivity pattern of glia-α1-specific T-cell clones to gliadin protein before (NC) and after mTG transamidation with Lys (mTG-Lys) and after tTG treatment of mTG-Lys (tTG-mTG-Lys). Three different concentrations of lysine were used, 10, 40, and 160 mM as indicated. (F) Reactivity pattern of glia-α2-specific T-cell clones to gliadin protein and its modified products.

Article Snippet: DQ2.5-glia-α1a (native form LQPFPQPQLPYPQ and deamidated form LQPFPQP E LPYAA), DQ2.5-glia-α2 (native form PFPQPQLPYPQPQ and deamidated form AAPQP E LPYPQPQ), and deamidated 33-mer (LQLQPF (PQP E LPY) 3 PQPQPF) peptides were synthesized by standard Fmoc chemistry on a multiple peptide synthesizer (Syroll, MultiSynTech GmbH, Witten, Germany).

Techniques: Clone Assay, Modification