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Addgene inc 321 tag codons
Bacterial strains and plasmids used in this study.
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Bacterial strains and plasmids used in this study.

Journal: Frontiers in Microbiology

Article Title: Comparative Analyses of the Transcriptome and Proteome of Escherichia coli C321.△A and Further Improving Its Noncanonical Amino Acids Containing Protein Expression Ability by Integration of T7 RNA Polymerase

doi: 10.3389/fmicb.2021.744284

Figure Lengend Snippet: Bacterial strains and plasmids used in this study.

Article Snippet: E. coli C321.ΔA , E. coli MG1655Δ ( ybhB-bioAB )::[λcI857 N( croea59 ):: tetR-bla ] Δ prfA Δ mutS :: zeoR ; all 321 TAG codons changed to TAA , Addgene (ID: 48998).

Techniques: Plasmid Preparation, CRISPR, Sequencing, Mutagenesis