3147001b Search Results


147 sm conjugated anti human cd20  (fluidigm)
94
fluidigm 147 sm conjugated anti human cd20
Surface marker antibody cocktail
147 Sm Conjugated Anti Human Cd20, supplied by fluidigm, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/3147001b/Anti-Human+CD20+(2H7)-147Sm/pmc08976126-5-0-9
Average 94 stars, based on 1 article reviews
147 sm conjugated anti human cd20 - by Bioz Stars, 2026-10
94/100 stars
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Surface marker antibody cocktail

Journal: STAR Protocols

Article Title: CyTOF mass cytometry analysis of human memory CD4 + T cells and memory B cells

doi: 10.1016/j.xpro.2022.101269

Figure Lengend Snippet: Surface marker antibody cocktail

Article Snippet: 147 Sm-conjugated anti-human CD20 (clone 2H7) (1:50 dilution) , Fluidigm , Cat#3147001B.

Techniques: Marker

CyTOF data analysis (A) PBMCs from four healthy donors were stained with a panel of metal-labeled antibodies and analyzed by CyTOF. After data pre-processing, manual gating was used to identify classical MBCs (CD3 - CD19 + CD20 + CD10 - CD27 + CD21 + ), atypical MBCs (CD3 - CD19 + CD20 + CD10 - CD27 - CD21 - ) and activated MBCs (CD3 - CD19 + CD20 + CD10 - CD27 + CD21 - ). tSNE analysis was then performed in Cytobank ( <xref ref-type=Kotecha et al., 2010 ) before self-organizing maps (SOMs) were generated using hierarchical consensus clustering on the tSNE axes. (B) tSNE and FlowSOM analysis of classical MBCs. (C) tSNE and FlowSOM analysis of atypical MBCs. (D) tSNE and FlowSOM analysis of activated MBCs. (E) A similar approach was used to analyze the memory CD4 + T cell compartment. Manual gating was used to identify T H1 memory CD4 + T cells (CD19 - CD3 + CD4 + CD45RA - CCR6 - CXCR3 + ), T H2 memory CD4 + T cells (CD19 - CD3 + CD4 + CD45RA - CCR6 - CXCR3 - ), T H17 memory CD4 + T cells (CD19 - CD3 + CD4 + CD45RA - CCR6 + CXCR3 - ) and circulating memory T FH cells (CD19 - CD3 + CD4 + CD45RA - CXCR5 + ) before tSNE analysis and FlowSOM clustering was performed for each memory CD4 + T cell sub-set. (F) tSNE and FlowSOM analysis of T H1 memory CD4 + T cells. (G) tSNE and FlowSOM analysis of T H2 memory CD4 + T cells. (H) tSNE and FlowSOM analysis of T H17 memory CD4 + T cells. (I) tSNE and FlowSOM analysis of circulating memory T FH cells. The tSNE plots in the upper left panel display cell density and represent the pooled data from four healthy donors, while the upper right panel shows a projection of the FlowSOM clusters on the tSNE plot. Heatmaps shows the mean marker expression for each FlowSOM cluster. " width="100%" height="100%">

Journal: STAR Protocols

Article Title: CyTOF mass cytometry analysis of human memory CD4 + T cells and memory B cells

doi: 10.1016/j.xpro.2022.101269

Figure Lengend Snippet: CyTOF data analysis (A) PBMCs from four healthy donors were stained with a panel of metal-labeled antibodies and analyzed by CyTOF. After data pre-processing, manual gating was used to identify classical MBCs (CD3 - CD19 + CD20 + CD10 - CD27 + CD21 + ), atypical MBCs (CD3 - CD19 + CD20 + CD10 - CD27 - CD21 - ) and activated MBCs (CD3 - CD19 + CD20 + CD10 - CD27 + CD21 - ). tSNE analysis was then performed in Cytobank ( Kotecha et al., 2010 ) before self-organizing maps (SOMs) were generated using hierarchical consensus clustering on the tSNE axes. (B) tSNE and FlowSOM analysis of classical MBCs. (C) tSNE and FlowSOM analysis of atypical MBCs. (D) tSNE and FlowSOM analysis of activated MBCs. (E) A similar approach was used to analyze the memory CD4 + T cell compartment. Manual gating was used to identify T H1 memory CD4 + T cells (CD19 - CD3 + CD4 + CD45RA - CCR6 - CXCR3 + ), T H2 memory CD4 + T cells (CD19 - CD3 + CD4 + CD45RA - CCR6 - CXCR3 - ), T H17 memory CD4 + T cells (CD19 - CD3 + CD4 + CD45RA - CCR6 + CXCR3 - ) and circulating memory T FH cells (CD19 - CD3 + CD4 + CD45RA - CXCR5 + ) before tSNE analysis and FlowSOM clustering was performed for each memory CD4 + T cell sub-set. (F) tSNE and FlowSOM analysis of T H1 memory CD4 + T cells. (G) tSNE and FlowSOM analysis of T H2 memory CD4 + T cells. (H) tSNE and FlowSOM analysis of T H17 memory CD4 + T cells. (I) tSNE and FlowSOM analysis of circulating memory T FH cells. The tSNE plots in the upper left panel display cell density and represent the pooled data from four healthy donors, while the upper right panel shows a projection of the FlowSOM clusters on the tSNE plot. Heatmaps shows the mean marker expression for each FlowSOM cluster.

Article Snippet: 147 Sm-conjugated anti-human CD20 (clone 2H7) (1:50 dilution) , Fluidigm , Cat#3147001B.

Techniques: Staining, Labeling, Generated, Marker, Expressing

Journal: STAR Protocols

Article Title: CyTOF mass cytometry analysis of human memory CD4 + T cells and memory B cells

doi: 10.1016/j.xpro.2022.101269

Figure Lengend Snippet:

Article Snippet: 147 Sm-conjugated anti-human CD20 (clone 2H7) (1:50 dilution) , Fluidigm , Cat#3147001B.

Techniques: Recombinant, Saline, Staining, Software, Cytometry