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parental human fibrosarcoma 2ftgh cells  (Dr Raymond Laboratories Inc)
90
Dr Raymond Laboratories Inc parental human fibrosarcoma 2ftgh cells
ZIKV infection induced OAS2 expression partially dependent on Type I IFN. <t>2FTGH</t> or U5A cells were infected ZIKV at MOI of 0, 5, 10 and 20. Total RNA were harvested at 48 h post ZIKV infection. The mRNA levels of selected genes were examined by RT-qPCR. ( A ) ZIKV can successfully infect and replicate in 2FTGH and U5A cells. ( B ) ZIKV infection increased OAS2 mRNA levels in 2FTGH cells. ( C ) ZIKV infection increased OAS2 mRNA levels in U5A cells. ( D ) ZIKV infection increased IFNβ mRNA in 2FTGH cells. ( E ) ZIKV infection increased IFNβ expression in U5A cells. ( F ) IFNβ treatment induced OAS2 expression in 2FTGH cells but not in U5A cells. 100 IU/mL IFNβ was added into 2FTGH and U5A cells, and cellular total RNAs were harvested at 48 h post ZIKV infection. The mRNA level of OAS2 was examined by RT-qPCR. ( G ) ZIKV infection increased RIG-I mRNA level in 2FTGH cells. ( H ) ZIKV infection increased RIG-I mRNA level in U5A cells. Data were normalized to GAPDH and presented as mean ± S.D. ns > 0.05; * p < 0.05; ** p < 0.01; *** p < 0.001 versus the control treatment.
Parental Human Fibrosarcoma 2ftgh Cells, supplied by Dr Raymond Laboratories Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/parental human fibrosarcoma 2ftgh cells/product/Dr Raymond Laboratories Inc
Average 90 stars, based on 1 article reviews
parental human fibrosarcoma 2ftgh cells - by Bioz Stars, 2026-05
90/100 stars
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The 2fTGH Cell Line is part of a panel of IFNy pathway mutant fibrosarcoma cell lines isolated by chemical mutagenesis of IFNy insensitive reporter cells derived from HT1080 cells. Knockout genes have been identified and
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ZIKV infection induced OAS2 expression partially dependent on Type I IFN. 2FTGH or U5A cells were infected ZIKV at MOI of 0, 5, 10 and 20. Total RNA were harvested at 48 h post ZIKV infection. The mRNA levels of selected genes were examined by RT-qPCR. ( A ) ZIKV can successfully infect and replicate in 2FTGH and U5A cells. ( B ) ZIKV infection increased OAS2 mRNA levels in 2FTGH cells. ( C ) ZIKV infection increased OAS2 mRNA levels in U5A cells. ( D ) ZIKV infection increased IFNβ mRNA in 2FTGH cells. ( E ) ZIKV infection increased IFNβ expression in U5A cells. ( F ) IFNβ treatment induced OAS2 expression in 2FTGH cells but not in U5A cells. 100 IU/mL IFNβ was added into 2FTGH and U5A cells, and cellular total RNAs were harvested at 48 h post ZIKV infection. The mRNA level of OAS2 was examined by RT-qPCR. ( G ) ZIKV infection increased RIG-I mRNA level in 2FTGH cells. ( H ) ZIKV infection increased RIG-I mRNA level in U5A cells. Data were normalized to GAPDH and presented as mean ± S.D. ns > 0.05; * p < 0.05; ** p < 0.01; *** p < 0.001 versus the control treatment.

Journal: Viruses

Article Title: 2′, 5′-Oligoadenylate Synthetase 2 (OAS2) Inhibits Zika Virus Replication through Activation of Type Ι IFN Signaling Pathway

doi: 10.3390/v12040418

Figure Lengend Snippet: ZIKV infection induced OAS2 expression partially dependent on Type I IFN. 2FTGH or U5A cells were infected ZIKV at MOI of 0, 5, 10 and 20. Total RNA were harvested at 48 h post ZIKV infection. The mRNA levels of selected genes were examined by RT-qPCR. ( A ) ZIKV can successfully infect and replicate in 2FTGH and U5A cells. ( B ) ZIKV infection increased OAS2 mRNA levels in 2FTGH cells. ( C ) ZIKV infection increased OAS2 mRNA levels in U5A cells. ( D ) ZIKV infection increased IFNβ mRNA in 2FTGH cells. ( E ) ZIKV infection increased IFNβ expression in U5A cells. ( F ) IFNβ treatment induced OAS2 expression in 2FTGH cells but not in U5A cells. 100 IU/mL IFNβ was added into 2FTGH and U5A cells, and cellular total RNAs were harvested at 48 h post ZIKV infection. The mRNA level of OAS2 was examined by RT-qPCR. ( G ) ZIKV infection increased RIG-I mRNA level in 2FTGH cells. ( H ) ZIKV infection increased RIG-I mRNA level in U5A cells. Data were normalized to GAPDH and presented as mean ± S.D. ns > 0.05; * p < 0.05; ** p < 0.01; *** p < 0.001 versus the control treatment.

Article Snippet: Hepatoma Huh7, Human IFNAR-deficient U5A cells and the parental human fibrosarcoma 2FTGH cells were kindly provided by Dr. Raymond Chang (Massachusetts General Hospital, USA).

Techniques: Infection, Expressing, Quantitative RT-PCR, Control