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Fig. 8. Comparison of IP10 secretion by circulating immune cells in vaccinated sheep based on disease outcome. Gudair vaccinated sheep exposed to MAP had blood collected at 4, 6 and 12 months post exposure for whole blood stimulation assays. Supernatants from the assays were then examined with an IP10 <t>ELISA</t> to assess the IP10 recall response. Sheep were retrospectively grouped based on infection status determined by culture of gut tissue 12 months after exposure. Data shown is predicted means and standard errors. * Denotes significant differences between groups (p < 0.05) determined using a restricted maximum likelihood linear mixed model.
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Fig. 8. Comparison of IP10 secretion by circulating immune cells in vaccinated sheep based on disease outcome. Gudair vaccinated sheep exposed to MAP had blood collected at 4, 6 and 12 months post exposure for whole blood stimulation assays. Supernatants from the assays were then examined with an IP10 <t>ELISA</t> to assess the IP10 recall response. Sheep were retrospectively grouped based on infection status determined by culture of gut tissue 12 months after exposure. Data shown is predicted means and standard errors. * Denotes significant differences between groups (p < 0.05) determined using a restricted maximum likelihood linear mixed model.
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Fig. 8. Comparison of IP10 secretion by circulating immune cells in vaccinated sheep based on disease outcome. Gudair vaccinated sheep exposed to MAP had blood collected at 4, 6 and 12 months post exposure for whole blood stimulation assays. Supernatants from the assays were then examined with an IP10 <t>ELISA</t> to assess the IP10 recall response. Sheep were retrospectively grouped based on infection status determined by culture of gut tissue 12 months after exposure. Data shown is predicted means and standard errors. * Denotes significant differences between groups (p < 0.05) determined using a restricted maximum likelihood linear mixed model.
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Fig. 8. Comparison of IP10 secretion by circulating immune cells in vaccinated sheep based on disease outcome. Gudair vaccinated sheep exposed to MAP had blood collected at 4, 6 and 12 months post exposure for whole blood stimulation assays. Supernatants from the assays were then examined with an IP10 <t>ELISA</t> to assess the IP10 recall response. Sheep were retrospectively grouped based on infection status determined by culture of gut tissue 12 months after exposure. Data shown is predicted means and standard errors. * Denotes significant differences between groups (p < 0.05) determined using a restricted maximum likelihood linear mixed model.
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Fig. 8. Comparison of IP10 secretion by circulating immune cells in vaccinated sheep based on disease outcome. Gudair vaccinated sheep exposed to MAP had blood collected at 4, 6 and 12 months post exposure for whole blood stimulation assays. Supernatants from the assays were then examined with an IP10 <t>ELISA</t> to assess the IP10 recall response. Sheep were retrospectively grouped based on infection status determined by culture of gut tissue 12 months after exposure. Data shown is predicted means and standard errors. * Denotes significant differences between groups (p < 0.05) determined using a restricted maximum likelihood linear mixed model.
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Fig. 8. Comparison of IP10 secretion by circulating immune cells in vaccinated sheep based on disease outcome. Gudair vaccinated sheep exposed to MAP had blood collected at 4, 6 and 12 months post exposure for whole blood stimulation assays. Supernatants from the assays were then examined with an IP10 <t>ELISA</t> to assess the IP10 recall response. Sheep were retrospectively grouped based on infection status determined by culture of gut tissue 12 months after exposure. Data shown is predicted means and standard errors. * Denotes significant differences between groups (p < 0.05) determined using a restricted maximum likelihood linear mixed model.
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Fig. 8. Comparison of IP10 secretion by circulating immune cells in vaccinated sheep based on disease outcome. Gudair vaccinated sheep exposed to MAP had blood collected at 4, 6 and 12 months post exposure for whole blood stimulation assays. Supernatants from the assays were then examined with an IP10 <t>ELISA</t> to assess the IP10 recall response. Sheep were retrospectively grouped based on infection status determined by culture of gut tissue 12 months after exposure. Data shown is predicted means and standard errors. * Denotes significant differences between groups (p < 0.05) determined using a restricted maximum likelihood linear mixed model.
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Fig. 8. Comparison of IP10 secretion by circulating immune cells in vaccinated sheep based on disease outcome. Gudair vaccinated sheep exposed to MAP had blood collected at 4, 6 and 12 months post exposure for whole blood stimulation assays. Supernatants from the assays were then examined with an IP10 <t>ELISA</t> to assess the IP10 recall response. Sheep were retrospectively grouped based on infection status determined by culture of gut tissue 12 months after exposure. Data shown is predicted means and standard errors. * Denotes significant differences between groups (p < 0.05) determined using a restricted maximum likelihood linear mixed model.
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Fig. 8. Comparison of IP10 secretion by circulating immune cells in vaccinated sheep based on disease outcome. Gudair vaccinated sheep exposed to MAP had blood collected at 4, 6 and 12 months post exposure for whole blood stimulation assays. Supernatants from the assays were then examined with an IP10 ELISA to assess the IP10 recall response. Sheep were retrospectively grouped based on infection status determined by culture of gut tissue 12 months after exposure. Data shown is predicted means and standard errors. * Denotes significant differences between groups (p < 0.05) determined using a restricted maximum likelihood linear mixed model.

Journal: Vaccine

Article Title: IP10 is a predictor of successful vaccine protection against paratuberculosis infection in sheep.

doi: 10.1016/j.vaccine.2022.11.018

Figure Lengend Snippet: Fig. 8. Comparison of IP10 secretion by circulating immune cells in vaccinated sheep based on disease outcome. Gudair vaccinated sheep exposed to MAP had blood collected at 4, 6 and 12 months post exposure for whole blood stimulation assays. Supernatants from the assays were then examined with an IP10 ELISA to assess the IP10 recall response. Sheep were retrospectively grouped based on infection status determined by culture of gut tissue 12 months after exposure. Data shown is predicted means and standard errors. * Denotes significant differences between groups (p < 0.05) determined using a restricted maximum likelihood linear mixed model.

Article Snippet: The IP-10 levels in stimulated whole blood samples were measured using the optimised protocol for the ovine IP10 do-ityourself ELISA (Kingfisher Biotech).

Techniques: Comparison, Enzyme-linked Immunosorbent Assay, Infection