Journal: Open Medicine

Article Title: WTAP affects intracranial aneurysm progression by regulating m6A methylation modification

doi: 10.1515/med-2023-0818

Figure Lengend Snippet: Roles of WTAP overexpression in the growth of the TNF-α-induced rBMVECs. (a) Cell viability of the rBMVECs, with different treatments, following 24, 48, and 72 h of culture. (b) Apoptosis of the rBMVECs, with different treatments, determined using flow cytometry. (c) Cleaved-caspase3 protein expression detected using western blotting. (d) WTAP protein expression detected using western blotting. * P < 0.05, as compared to the samples from the control cells; # P < 0.05, as compared to those from the TNF-α cells.

Article Snippet: Following 2 h of incubation, the primary antibodies, including the anti-WTAP antibody (1:5,000, Proteintech Group, Inc.), anti-cleaved caspase3 (1:1,000, Proteintech Group, Inc.), and anti-GAPDH antibody (1:1,000, Proteintech Group, Inc.), were added to the membranes.

Techniques: Over Expression, Flow Cytometry, Expressing, Western Blot, Control