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Image Search Results
Journal: Molecular neurobiology
Article Title: Citalopram reduces aggregation of ATXN3 in a YAC transgenic mouse model of Machado-Joseph disease
doi: 10.1007/s12035-018-1331-2
Figure Lengend Snippet: Assessment of macroautophagy markers shows increased levels of p62 in the spinal cord (B), and of p62 and beclin-1 in the cerebellum (C) of citalopram-treated mice compared to controls. Citalopram treatment did not affect levels of LC3 in any of the three regions (A-C). (A, B, and C) Left panels show immunoblots of indicated proteins in soluble fractions of brainstem, spinal cord and cerebellum. Right panels display quantification of band intensity, with values normalized to Gapdh. Bars represent the average percentage of protein relative to vehicle-treated mice (± SEM). Statistical significance is indicated as *P < 0.05 and **P < 0.01. Mice 1, 2, and 20 were excluded from statistical analysis. Diamond (◆) represents p62 bands.
Article Snippet: Total protein lysates from soluble fractions (100 μg from brainstem, and cerebellum) were resolved on 10% SDS-PAGE gels, and corresponding PVDF membranes were incubated overnight at 4 °C with primary antibodies: mouse anti-HSP70 (1:500; SPA810, Enzo Life Sciences), rabbit anti-HSP90α (1:1000; ab2928, Abcam), mouse anti-HSP90β (1:1000; ADI-SPA842, Enzo Life Sciences), rabbit anti-HSP40 (1:1000; #4868, Cell Signaling),
Techniques: Western Blot
Journal: Cancer cell
Article Title: KRAS-IRF2 axis drives immune suppression and immune therapy resistance in colorectal cancer
doi: 10.1016/j.ccell.2019.02.008
Figure Lengend Snippet: KEY RESOURCES TABLE
Article Snippet:
Techniques: Control, Labeling, Recombinant, Enzyme-linked Immunosorbent Assay, Isolation, Cell Isolation, Bicinchoninic Acid Protein Assay, cDNA Synthesis, Microarray, shRNA, Plasmid Preparation, Software, Blocking Assay, Transfection, Staining