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Image Search Results
Journal: ACS infectious diseases
Article Title: Detection of "Hidden" Antimicrobial Drug Resistance.
doi: 10.1021/acsinfecdis.9b00132
Figure Lengend Snippet: Figure 3 Growth of E coli strains and other clinically isolated infectious bacteria: (Upper- panel A-L): growth curve tracked by EZMTT method; (Lower-panel a-l): growth curve tracked by turbidity method; doubling times at the log phase measured by the EZMTT and by the turbidity methods were A) 0.80±0.11h and 0.79±0.10 h for DH5α, respectively; B) 1.4±0.4 h and 1.6±0.3 h for MG1655 (wild type E. coli), respectively; C) 0.42±0.06 h and 0.42±0.08 h for EAEC ( adhesive E. coli), respectively; D) 0.40±0.11 h and 0.40±0.07 h for EIEC (invasive E. coli); E) 0.43±0.09 h and 0.42±0.09 h for EPEC (pathogenic E. coli), F) 0.41±0.05 h and 0.40±0.07 h for ETEC (toxigenic E. coli), respectively; G) 0.72±0.23 h and 0.74±0.06 h for Salmonella, respectively; H) 0.80±0.10 h and 0.71±0.08 h for Salmonella paratyphi, respectively; I) 1.33±0.14 h and 0.82±0.20 h for Shigella flexneri, respectively; J) 1.17±0.02 h and 1.30±0.27 h for Shigella sonnei, respectively; K) 0.50±0.07 h and 0.49±0.06 h for Staphylococcus aureus, respectively; L) 0.42±0.08 h and 0.47±0.18 h for Bacillus cereus, respectively.
Article Snippet: Mueller-Hinton Broth was purchased from OXOID(Hampshire,U.K;Chemicals from Sigma (USA), EZMTT purchased from JNF Bioscience (China; USA), CCK-8 or WST-8 from Beyotime Biotechnology (China), Enterococcus casseliflavus Collins et al. ATCC700327,
Techniques: Isolation, Bacteria, Adhesive
Journal: ACS infectious diseases
Article Title: Detection of "Hidden" Antimicrobial Drug Resistance.
doi: 10.1021/acsinfecdis.9b00132
Figure Lengend Snippet: Figure 5. AST of antibiotics in clinically isolated A) E. coli (B) Staphylococcus aureus and (C) Bacillus cereus strains (2 10 CFU/ml) using the turbidity and the EZMTT methods; (AMP, KAN, GEN) DIP curves collected by the turbidity and the EZMTT methods; (5 hrs, 16 hrs) z-factors measured using the absorbance of OD750 nm in the turbidity assay and the absorbance of OD450 nm in the EZMTT assay at both the 5 hrs and the 16 hrs time points, respectively (z factor > 0.5 indicates excellent reproducibility; calculation z =1-[(3SD of high control)+(3SD of low control)]/[(mean of high control)-(mean of low control)]); (IC50) IC50 curves collected at the 24 hrs time point by the turbidity (black curve), the EZMTT (red curve) and the MTT methods (blue curve), showing essentially the same AST results, but the EZMTT assay showed much better assay precision.
Article Snippet: Mueller-Hinton Broth was purchased from OXOID(Hampshire,U.K;Chemicals from Sigma (USA), EZMTT purchased from JNF Bioscience (China; USA), CCK-8 or WST-8 from Beyotime Biotechnology (China), Enterococcus casseliflavus Collins et al. ATCC700327,
Techniques: Isolation, Control
Journal: ACS infectious diseases
Article Title: Detection of "Hidden" Antimicrobial Drug Resistance.
doi: 10.1021/acsinfecdis.9b00132
Figure Lengend Snippet: Figure 6. DIP curves collected by the turbidity (OD600nm) and the EZMTT (OD450nm) methods, showing the growth inhibitions of the wild type E. coli (MG1655) and the clinically isolated E. coli strains (EAEC, EIEC, EPEC, ETEC, A) by 12 antibiotic medicine (Fig S1A, S1B). Results are representative of at least 3 experiments in duplicates.
Article Snippet: Mueller-Hinton Broth was purchased from OXOID(Hampshire,U.K;Chemicals from Sigma (USA), EZMTT purchased from JNF Bioscience (China; USA), CCK-8 or WST-8 from Beyotime Biotechnology (China), Enterococcus casseliflavus Collins et al. ATCC700327,
Techniques: Isolation