24169 1 ap Search Results


atf6  (Proteintech)
96
Proteintech atf6
Extraction from CT decreases UVA-induced ER stress in HaCaT cells. HaCaT cells were subjected to UVA (5 J/cm 2 ) radiation and then treated with the indicated concentrations of extraction from CT. (A) Western blot analysis was performed to determine the protein levels of phosphorylated IRE1 (pIRE1), <t>ATF6,</t> phosphorylated PERK (p-PERK), phosphorylated eIF2a (p-eIF2a) and CHOP in different treatments. (B) Immunofluorescence assay was performed to detect CHOP expression. (C) Representative images showing the ER morphology in control, UVA-treated and CT-treated cells with UVA exposure. Cells were stained with ER tracker in green and counterstained with DAPI for nuclear visualization. N = 4. * p ≤ 0.05; ** p ≤ 0.01, *** p ≤ 0.001.
Atf6, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/24169+1+ap/ATF6+Antibody/pmc12741067-101-25-26
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atf6 - by Bioz Stars, 2026-09
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Extraction from CT decreases UVA-induced ER stress in HaCaT cells. HaCaT cells were subjected to UVA (5 J/cm 2 ) radiation and then treated with the indicated concentrations of extraction from CT. (A) Western blot analysis was performed to determine the protein levels of phosphorylated IRE1 (pIRE1), ATF6, phosphorylated PERK (p-PERK), phosphorylated eIF2a (p-eIF2a) and CHOP in different treatments. (B) Immunofluorescence assay was performed to detect CHOP expression. (C) Representative images showing the ER morphology in control, UVA-treated and CT-treated cells with UVA exposure. Cells were stained with ER tracker in green and counterstained with DAPI for nuclear visualization. N = 4. * p ≤ 0.05; ** p ≤ 0.01, *** p ≤ 0.001.

Journal: Frontiers in Pharmacology

Article Title: Coreopsis tinctoria Nutt. attenuates ultraviolet A photodamage by suppressing endoplasmic reticulum stress-induced apoptosis via Nrf2 crosstalk

doi: 10.3389/fphar.2025.1686234

Figure Lengend Snippet: Extraction from CT decreases UVA-induced ER stress in HaCaT cells. HaCaT cells were subjected to UVA (5 J/cm 2 ) radiation and then treated with the indicated concentrations of extraction from CT. (A) Western blot analysis was performed to determine the protein levels of phosphorylated IRE1 (pIRE1), ATF6, phosphorylated PERK (p-PERK), phosphorylated eIF2a (p-eIF2a) and CHOP in different treatments. (B) Immunofluorescence assay was performed to detect CHOP expression. (C) Representative images showing the ER morphology in control, UVA-treated and CT-treated cells with UVA exposure. Cells were stained with ER tracker in green and counterstained with DAPI for nuclear visualization. N = 4. * p ≤ 0.05; ** p ≤ 0.01, *** p ≤ 0.001.

Article Snippet: The membranes were blocked with 5% non-fat milk and incubated with the following primary antibodies: IRE1 (phospho S724) (Abcam, ab124945); CHOP (Proteintech,15204-1-AP); NRF2 (Proteintech, 16396-1-AP); ATF6 (Proteintech, 24169-1-AP); PERK (phospho Thr982) (Proteintech, 82534-1-RR); eIF2α(phospho Ser51) (Proteintech, 68023-1-Ig); GADD34 (Proteintech, 10449-1-AP); Caspase9 (Proteintech, 10380-1-AP); Cleaved caspase3 (Proteintech, 25128-1-AP); JNK (Proteintech, 51153-1-AP); JNK (phospho Tyr185) (Proteintech, 80024-1-RR); Bax (Proteintech, 50599-2-Ig); BCL2 (Proteintech, 60178-1-Ig); HO-1 (Proteintech, 10701-1-AP); β-Actin (Proteintech, 20536-1-AP); Horseradish peroxidase (HRP)-conjugated goat anti-rabbit and anti-mouse secondary antibodies were applied (Jackson, 111-035-003 and 115-035-003).

Techniques: Extraction, Western Blot, Immunofluorescence, Expressing, Control, Staining

CT alleviates UVA-induced ER stress and apoptosis via Nrf2 in HaCaT cells. (A) HaCaT cells were subjected to UVA radiation and treated with 50 or 100 μg/mL CT and/or siNrf2. Western blot assays were performed to detect the levels of ATF6, phosphorylated elF2a (p-elF2a), CHOP, GADD34, Cleaved caspase9, Cleaved caspase3, JNK, phosphorylated JNK (p-JNK), Bax, BCL2, cytoplasmic and nuclear NRF2 and HO-1. (B) Flow cytometry analysis was performed to determine cell apoptosis. Quantification of the percentage of apoptotic cells in different treatments. (C) Heatmap generated the data from RNAseq. Genes are all related to NRF2 downstream anti-oxidant or detoxification target genes. The figure was generated by SRplot . N = 4. * p ≤ 0.05; ** p ≤ 0.01, *** p ≤ 0.001, **** p ≤ 0.0001.

Journal: Frontiers in Pharmacology

Article Title: Coreopsis tinctoria Nutt. attenuates ultraviolet A photodamage by suppressing endoplasmic reticulum stress-induced apoptosis via Nrf2 crosstalk

doi: 10.3389/fphar.2025.1686234

Figure Lengend Snippet: CT alleviates UVA-induced ER stress and apoptosis via Nrf2 in HaCaT cells. (A) HaCaT cells were subjected to UVA radiation and treated with 50 or 100 μg/mL CT and/or siNrf2. Western blot assays were performed to detect the levels of ATF6, phosphorylated elF2a (p-elF2a), CHOP, GADD34, Cleaved caspase9, Cleaved caspase3, JNK, phosphorylated JNK (p-JNK), Bax, BCL2, cytoplasmic and nuclear NRF2 and HO-1. (B) Flow cytometry analysis was performed to determine cell apoptosis. Quantification of the percentage of apoptotic cells in different treatments. (C) Heatmap generated the data from RNAseq. Genes are all related to NRF2 downstream anti-oxidant or detoxification target genes. The figure was generated by SRplot . N = 4. * p ≤ 0.05; ** p ≤ 0.01, *** p ≤ 0.001, **** p ≤ 0.0001.

Article Snippet: The membranes were blocked with 5% non-fat milk and incubated with the following primary antibodies: IRE1 (phospho S724) (Abcam, ab124945); CHOP (Proteintech,15204-1-AP); NRF2 (Proteintech, 16396-1-AP); ATF6 (Proteintech, 24169-1-AP); PERK (phospho Thr982) (Proteintech, 82534-1-RR); eIF2α(phospho Ser51) (Proteintech, 68023-1-Ig); GADD34 (Proteintech, 10449-1-AP); Caspase9 (Proteintech, 10380-1-AP); Cleaved caspase3 (Proteintech, 25128-1-AP); JNK (Proteintech, 51153-1-AP); JNK (phospho Tyr185) (Proteintech, 80024-1-RR); Bax (Proteintech, 50599-2-Ig); BCL2 (Proteintech, 60178-1-Ig); HO-1 (Proteintech, 10701-1-AP); β-Actin (Proteintech, 20536-1-AP); Horseradish peroxidase (HRP)-conjugated goat anti-rabbit and anti-mouse secondary antibodies were applied (Jackson, 111-035-003 and 115-035-003).

Techniques: Western Blot, Flow Cytometry, Generated