229e strain Search Results


93
ATCC human coronavirus 229e
Analytical specificity evaluation results of the Kaira assay.
Human Coronavirus 229e, supplied by ATCC, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 93 stars, based on 1 article reviews
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96
ATCC strain 229e
FIG. 4. Inhibition of coronavirus replication by 4E2RCat. (A) Coronavirus replication is eIF4F dependent. Following infection and exposure to either vehicle (0.2% DMSO) or the indicated concentrations of hippuristanol, silvestrol, 4E1RCat, or 4E2RCat, infectious viral titers released from the cells (extracellular) were determined, and the averages from three independent experiments are presented. Error bars denote standard errors of the means. LOD, limit of detection (denoted by the red dashed line). (B) Effects of eIF4A and eIF4E-eIF4G inhibition on intracellular infectious virus production. (C) Effects of eIF4A and eIF4E-eIF4G inhibition on the production of <t>HCoV-229E</t> S protein. Following infection and exposure to vehicle or compounds for 24 h, infected cells were processed for immunofluorescence using a mouse IgG1 MAb, 5-11H.6, followed by AlexaFluor-488 anti-mouse goat antibody (green). Nuclei are stained with DAPI (blue). (D) Percent S protein-positive cells in HCoV-229E- infected cells.
Strain 229e, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 96 stars, based on 1 article reviews
strain 229e - by Bioz Stars, 2026-05
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90
ZeptoMetrix corporation culture fluid zeptometrix 0810229cf hi 1 04e 05 lod lod coronavirus
FIG. 4. Inhibition of coronavirus replication by 4E2RCat. (A) Coronavirus replication is eIF4F dependent. Following infection and exposure to either vehicle (0.2% DMSO) or the indicated concentrations of hippuristanol, silvestrol, 4E1RCat, or 4E2RCat, infectious viral titers released from the cells (extracellular) were determined, and the averages from three independent experiments are presented. Error bars denote standard errors of the means. LOD, limit of detection (denoted by the red dashed line). (B) Effects of eIF4A and eIF4E-eIF4G inhibition on intracellular infectious virus production. (C) Effects of eIF4A and eIF4E-eIF4G inhibition on the production of <t>HCoV-229E</t> S protein. Following infection and exposure to vehicle or compounds for 24 h, infected cells were processed for immunofluorescence using a mouse IgG1 MAb, 5-11H.6, followed by AlexaFluor-488 anti-mouse goat antibody (green). Nuclei are stained with DAPI (blue). (D) Percent S protein-positive cells in HCoV-229E- infected cells.
Culture Fluid Zeptometrix 0810229cf Hi 1 04e 05 Lod Lod Coronavirus, supplied by ZeptoMetrix corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Bilthoven Biologicals coronavirus 229e reference strains
FIG. 4. Inhibition of coronavirus replication by 4E2RCat. (A) Coronavirus replication is eIF4F dependent. Following infection and exposure to either vehicle (0.2% DMSO) or the indicated concentrations of hippuristanol, silvestrol, 4E1RCat, or 4E2RCat, infectious viral titers released from the cells (extracellular) were determined, and the averages from three independent experiments are presented. Error bars denote standard errors of the means. LOD, limit of detection (denoted by the red dashed line). (B) Effects of eIF4A and eIF4E-eIF4G inhibition on intracellular infectious virus production. (C) Effects of eIF4A and eIF4E-eIF4G inhibition on the production of <t>HCoV-229E</t> S protein. Following infection and exposure to vehicle or compounds for 24 h, infected cells were processed for immunofluorescence using a mouse IgG1 MAb, 5-11H.6, followed by AlexaFluor-488 anti-mouse goat antibody (green). Nuclei are stained with DAPI (blue). (D) Percent S protein-positive cells in HCoV-229E- infected cells.
Coronavirus 229e Reference Strains, supplied by Bilthoven Biologicals, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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90
National Centre for Cell Science ochrobactrum intermedium strain 229e
FIG. 4. Inhibition of coronavirus replication by 4E2RCat. (A) Coronavirus replication is eIF4F dependent. Following infection and exposure to either vehicle (0.2% DMSO) or the indicated concentrations of hippuristanol, silvestrol, 4E1RCat, or 4E2RCat, infectious viral titers released from the cells (extracellular) were determined, and the averages from three independent experiments are presented. Error bars denote standard errors of the means. LOD, limit of detection (denoted by the red dashed line). (B) Effects of eIF4A and eIF4E-eIF4G inhibition on intracellular infectious virus production. (C) Effects of eIF4A and eIF4E-eIF4G inhibition on the production of <t>HCoV-229E</t> S protein. Following infection and exposure to vehicle or compounds for 24 h, infected cells were processed for immunofluorescence using a mouse IgG1 MAb, 5-11H.6, followed by AlexaFluor-488 anti-mouse goat antibody (green). Nuclei are stained with DAPI (blue). (D) Percent S protein-positive cells in HCoV-229E- infected cells.
Ochrobactrum Intermedium Strain 229e, supplied by National Centre for Cell Science, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Mikrogen Diagnostik recombinant n-protein of the hcov strains hku1, oc43, nl63, and 229e
FIG. 4. Inhibition of coronavirus replication by 4E2RCat. (A) Coronavirus replication is eIF4F dependent. Following infection and exposure to either vehicle (0.2% DMSO) or the indicated concentrations of hippuristanol, silvestrol, 4E1RCat, or 4E2RCat, infectious viral titers released from the cells (extracellular) were determined, and the averages from three independent experiments are presented. Error bars denote standard errors of the means. LOD, limit of detection (denoted by the red dashed line). (B) Effects of eIF4A and eIF4E-eIF4G inhibition on intracellular infectious virus production. (C) Effects of eIF4A and eIF4E-eIF4G inhibition on the production of <t>HCoV-229E</t> S protein. Following infection and exposure to vehicle or compounds for 24 h, infected cells were processed for immunofluorescence using a mouse IgG1 MAb, 5-11H.6, followed by AlexaFluor-488 anti-mouse goat antibody (green). Nuclei are stained with DAPI (blue). (D) Percent S protein-positive cells in HCoV-229E- infected cells.
Recombinant N Protein Of The Hcov Strains Hku1, Oc43, Nl63, And 229e, supplied by Mikrogen Diagnostik, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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N/A
Please consult your institution’s regulations regarding the use of this product. For a detailed discussion on biological safety see the current edition of Biosafety in Microbiological and Biomedical Laboratories (BMBL), published by the CDC.
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Image Search Results


Analytical specificity evaluation results of the Kaira assay.

Journal: PLOS ONE

Article Title: Evaluation of the Kaira COVID-19/Flu/RSV Detection Kit for detection of SARS-CoV-2, influenza A/B, and respiratory syncytial virus: A comparative study with the PowerChek SARS-CoV-2, influenza A&B, RSV Multiplex Real-time PCR Kit

doi: 10.1371/journal.pone.0278530

Figure Lengend Snippet: Analytical specificity evaluation results of the Kaira assay.

Article Snippet: Human coronavirus 229E , ATCC (VR-740D) , Negative.

Techniques: Virus

FIG. 4. Inhibition of coronavirus replication by 4E2RCat. (A) Coronavirus replication is eIF4F dependent. Following infection and exposure to either vehicle (0.2% DMSO) or the indicated concentrations of hippuristanol, silvestrol, 4E1RCat, or 4E2RCat, infectious viral titers released from the cells (extracellular) were determined, and the averages from three independent experiments are presented. Error bars denote standard errors of the means. LOD, limit of detection (denoted by the red dashed line). (B) Effects of eIF4A and eIF4E-eIF4G inhibition on intracellular infectious virus production. (C) Effects of eIF4A and eIF4E-eIF4G inhibition on the production of HCoV-229E S protein. Following infection and exposure to vehicle or compounds for 24 h, infected cells were processed for immunofluorescence using a mouse IgG1 MAb, 5-11H.6, followed by AlexaFluor-488 anti-mouse goat antibody (green). Nuclei are stained with DAPI (blue). (D) Percent S protein-positive cells in HCoV-229E- infected cells.

Journal: Journal of Virology

Article Title: Blocking eIF4E-eIF4G Interaction as a Strategy To Impair Coronavirus Replication

doi: 10.1128/jvi.00078-11

Figure Lengend Snippet: FIG. 4. Inhibition of coronavirus replication by 4E2RCat. (A) Coronavirus replication is eIF4F dependent. Following infection and exposure to either vehicle (0.2% DMSO) or the indicated concentrations of hippuristanol, silvestrol, 4E1RCat, or 4E2RCat, infectious viral titers released from the cells (extracellular) were determined, and the averages from three independent experiments are presented. Error bars denote standard errors of the means. LOD, limit of detection (denoted by the red dashed line). (B) Effects of eIF4A and eIF4E-eIF4G inhibition on intracellular infectious virus production. (C) Effects of eIF4A and eIF4E-eIF4G inhibition on the production of HCoV-229E S protein. Following infection and exposure to vehicle or compounds for 24 h, infected cells were processed for immunofluorescence using a mouse IgG1 MAb, 5-11H.6, followed by AlexaFluor-488 anti-mouse goat antibody (green). Nuclei are stained with DAPI (blue). (D) Percent S protein-positive cells in HCoV-229E- infected cells.

Article Snippet: Strain 229E of human coronavirus (HCoV-229E) originally was obtained from the American Type Culture Collection (ATCC) and was grown in the human L132 cell line (ATCC CCL5), which was expanded in alpha minimum essential medium ( -MEM) supplemented with 10% (vol/vol) fetal bovine serum (FBS).

Techniques: Inhibition, Infection, Virus, Staining

FIG. 5. Effects of 4E2RCat on coronavirus infection. (A) 4E2RCat inhibits coronavirus replication in a dose- and time-dependent manner. Following the exposure of HCoV-229E-infected L132 cells to the indicated concentrations of 4E2RCat for the indicated periods of time, infectious viral titers released from the cells (extracellular) or from intracellular infectious virus were determined, and the averages from three independent experiments are presented. Error bars denote SEM. The limit of detection is denoted by the red dashed line. (B) Dose-dependent inhibition of HCoV-229E S protein production by 4E2RCat. Following infection and exposure to vehicle or the indicated concentrations of 4E2RCat for 24 h, infected cells were processed for immunofluorescence using mouse IgG1 MAb 5-11H.6 followed by AlexaFluor-488 anti-mouse goat antibody (green). Nuclei are stained with DAPI (blue). (C) Metabolic labeling of HeLa cells infected with type 1 poliovirus (Mahoney) followed by treatment with vehicle (1% DMSO) or 50 M 4E2RCat for 4 h.

Journal: Journal of Virology

Article Title: Blocking eIF4E-eIF4G Interaction as a Strategy To Impair Coronavirus Replication

doi: 10.1128/jvi.00078-11

Figure Lengend Snippet: FIG. 5. Effects of 4E2RCat on coronavirus infection. (A) 4E2RCat inhibits coronavirus replication in a dose- and time-dependent manner. Following the exposure of HCoV-229E-infected L132 cells to the indicated concentrations of 4E2RCat for the indicated periods of time, infectious viral titers released from the cells (extracellular) or from intracellular infectious virus were determined, and the averages from three independent experiments are presented. Error bars denote SEM. The limit of detection is denoted by the red dashed line. (B) Dose-dependent inhibition of HCoV-229E S protein production by 4E2RCat. Following infection and exposure to vehicle or the indicated concentrations of 4E2RCat for 24 h, infected cells were processed for immunofluorescence using mouse IgG1 MAb 5-11H.6 followed by AlexaFluor-488 anti-mouse goat antibody (green). Nuclei are stained with DAPI (blue). (C) Metabolic labeling of HeLa cells infected with type 1 poliovirus (Mahoney) followed by treatment with vehicle (1% DMSO) or 50 M 4E2RCat for 4 h.

Article Snippet: Strain 229E of human coronavirus (HCoV-229E) originally was obtained from the American Type Culture Collection (ATCC) and was grown in the human L132 cell line (ATCC CCL5), which was expanded in alpha minimum essential medium ( -MEM) supplemented with 10% (vol/vol) fetal bovine serum (FBS).

Techniques: Infection, Virus, Inhibition, Staining, Labeling