Journal: European Journal of Microbiology & Immunology

Article Title: Phytochemicals and micronutrients in suppressing infectivity caused by SARS-CoV-2 virions and seasonal coronavirus HCoV-229E in vivo

doi: 10.1556/1886.2023.00010

Figure Lengend Snippet: Effect of test composition on weight change, viral load and spike protein in lung tissue infected and re-infected with human coronavirus 229E (HCoV-229E) . A. Effect of four-days of oral administration of MixV in K18-hACE2 mice with no significant weight change after 3 dpi and 5 dpi and oral administration of MixV with mid-term re-infection. B. The viral load in lung tissue after 3 dpi with oral administration of MixV and 5 dpi with oral administration and mid-term re-infection, determined by RT-qPCR as described in Materials and Methods section. C. Spike protein detection by WB in representative lung tissue samples. D. Quantification of spike protein performed as described in Materials and Methods section. Data are presented as mean ± SD; infected untreated animal n = 8, infected treated animal n = 8, re-infected untreated animal n = 8, re-infected treated animal n = 8; # P ≤ 0.05, ∆ P ≤ 0.01, dpi - days post-infection

Article Snippet: HCoV-229E anti-spike antibody was acquired from R&D Systems (Minneapolis, MN, USA).

Techniques: Infection, Quantitative RT-PCR

Journal: eLife

Article Title: Murine alveolar macrophages rapidly accumulate intranasally administered SARS-CoV-2 Spike protein leading to neutrophil recruitment and damage

doi: 10.7554/eLife.86764

Figure Lengend Snippet:

Article Snippet: Recombinant DNA reagent , Human coronavirus (HCoV-229E) Spike Gene ORF cDNA clone expression plasmid (Codon Optimized) HCoV-229E , SinoBiological , VG40605-UT , .

Techniques: Blocking Assay, Imaging, Staining, Recombinant, Plasmid Preparation, Expressing, Luciferase, Cell Culture, Transfection