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Image Search Results
Journal: Journal of Lipid Research
Article Title: A validated HPLC-MS/MS method for the simultaneous determination of ecdysteroid hormones in subminimal amounts of biological material
doi: 10.1016/j.jlr.2024.100640
Figure Lengend Snippet: Graphical diagram of the sample preparation workflow for ecdysteroid analysis. The detailed conditions are shown for the preparation of 1 μl of insect hemolymph. The sample preparation protocol includes three main steps: (a) extraction from biological material; (b) derivatization of the main ecdysteroid 20-hydroxyecdysone (20E) with hydroxylamine; and PT-SPE purification with final MRM HPLC-MS/MS analysis on a triple quadrupole MS. The experimental conditions and MRM transitions for the 20E-oxime derivative are shown. PT-SPE, pipette tip solid-phase extraction; MRM, multiple reaction monitoring.
Article Snippet:
Techniques: Sample Prep, Extraction, Purification, Tandem Mass Spectroscopy, Targeted Proteomics, Transferring
Journal: Journal of Lipid Research
Article Title: A validated HPLC-MS/MS method for the simultaneous determination of ecdysteroid hormones in subminimal amounts of biological material
doi: 10.1016/j.jlr.2024.100640
Figure Lengend Snippet: MRM transitions of all quantified ecdysteroids oxime derivatives, including precursor ion, product ions with collision energy, and retention time
Article Snippet:
Techniques:
Journal: Journal of Lipid Research
Article Title: A validated HPLC-MS/MS method for the simultaneous determination of ecdysteroid hormones in subminimal amounts of biological material
doi: 10.1016/j.jlr.2024.100640
Figure Lengend Snippet: Validation parameters (for n = 5) measured and calculated for method validation according to FDA guidelines
Article Snippet:
Techniques: Biomarker Discovery, Concentration Assay
Journal: Journal of Lipid Research
Article Title: A validated HPLC-MS/MS method for the simultaneous determination of ecdysteroid hormones in subminimal amounts of biological material
doi: 10.1016/j.jlr.2024.100640
Figure Lengend Snippet: MRM LC-MS/MS chromatograms for all ecdysteroid oximes studied. The chromatograms shown were used for quantification, and the relevant transitions, including the collision energy [eV], are also indicated. A: Standard mixture of ecdysteroids with two internal standards, ajugasterone C (AjugC), and taxisterone (Tax). B: 20-hydroxyecdysone (20E) and makisterone A (MaA), analytes from the hemolymph of Drosophila melanogaster . C: 20E, ecdysone (E), and 2-deoxyecdyosone (2dE) found in the larva of Ips typographus (bark beetle) at the concentrations described. D: 20E, E, and 2dE in the mosquito Culex quinquefasciatus at the concentrations indicated. E: Chromatograms obtained for 20E and concentrations measured in human urine and plasma after oral ingestion of maral root (ecdysteroid-rich plant). MRM, multiple reaction monitoring; 2dE, 2-deoxyecdysone; E, ecdysone.
Article Snippet:
Techniques: Liquid Chromatography with Mass Spectroscopy, Clinical Proteomics, Targeted Proteomics
Journal: Journal of Lipid Research
Article Title: A validated HPLC-MS/MS method for the simultaneous determination of ecdysteroid hormones in subminimal amounts of biological material
doi: 10.1016/j.jlr.2024.100640
Figure Lengend Snippet: Ecdysteroid contents in different insect species and types of samples measured during the study
Article Snippet:
Techniques: Two-Dimensional Gel Electrophoresis
Journal: Journal of Lipid Research
Article Title: A validated HPLC-MS/MS method for the simultaneous determination of ecdysteroid hormones in subminimal amounts of biological material
doi: 10.1016/j.jlr.2024.100640
Figure Lengend Snippet: Concentration values of 20E in human plasma, urine, and capillary blood from a finger after 14-day oral ingestion of a dietary supplement from maral root
Article Snippet:
Techniques: Concentration Assay, Clinical Proteomics
Journal: Journal of Lipid Research
Article Title: A validated HPLC-MS/MS method for the simultaneous determination of ecdysteroid hormones in subminimal amounts of biological material
doi: 10.1016/j.jlr.2024.100640
Figure Lengend Snippet: Overview of the analytical methods reported for the quantification of ecdysteroids in animals
Article Snippet:
Techniques: Extraction, Enzyme-linked Immunosorbent Assay, Injection, Targeted Proteomics, Clinical Proteomics