Journal: Frontiers in Microbiology

Article Title: Mucosal Prevalence and Interactions with the Epithelium Indicate Commensalism of Sutterella spp.

doi: 10.3389/fmicb.2016.01706

Figure Lengend Snippet: Adherence of S. wadsworthensis to 8-day-old Caco-2 and HT-29 cells in the competition and displacement assays.

Article Snippet: Sutterella wadsworthensis (DSM 14016 = ATCC 51579, type strain), S. parvirubra (DSM 19354 = YIT 11816 T , type strain) and S. stercoricanis (DSM 17807 = CCUG 47620 T , type strain) were obtained from German Collection of Microorganisms and Cell Cultures (DSMZ) and grown on Gifu anaerobic medium (GAM; Nissui Pharmaceutical Co., Ltd., Japan) agar for 2 days at 37°C in an anaerobic incubator (Concept Plus anaerobic workstation, Ruskinn Technology Ltd., UK) containing 85% N 2 , 10% CO 2 , and 5% H 2 .

Techniques:

Journal: Frontiers in Microbiology

Article Title: Mucosal Prevalence and Interactions with the Epithelium Indicate Commensalism of Sutterella spp.

doi: 10.3389/fmicb.2016.01706

Figure Lengend Snippet: IL-8 production in HT-29 cells induced by bacterial cell suspensions and LPS preparations. (A) IL-8 response in HT-29 cells by S. wadsworthensis , S. parvirubra , S. stercoricanis , B. fragilis (positive control), and E. coli (negative control) with 1:10, 1:100, and 1:1,000 dilutions from OD 600 nm 0.25 adjusted cell suspensions. The 1:10 dilution is not shown for E. coli due to excessive toxicity to the HT-29 cells. (B) IL-8 production induced by 1:1,000 and 1:10,000 dilutions of LPS preparations from E. coli and Sutterella spp. LPS preparations were made from the OD 600 nm 0.25 normalized cell suspensions and the dilutions were thereof. Results from the representative experiment are shown as means and standard deviations of three technical replicates (parallel wells). The culture medium was used as a background control in both assays. An asterisk indicates a significant ( ∗ p < 0.05) IL-8 production above the background level.

Article Snippet: Sutterella wadsworthensis (DSM 14016 = ATCC 51579, type strain), S. parvirubra (DSM 19354 = YIT 11816 T , type strain) and S. stercoricanis (DSM 17807 = CCUG 47620 T , type strain) were obtained from German Collection of Microorganisms and Cell Cultures (DSMZ) and grown on Gifu anaerobic medium (GAM; Nissui Pharmaceutical Co., Ltd., Japan) agar for 2 days at 37°C in an anaerobic incubator (Concept Plus anaerobic workstation, Ruskinn Technology Ltd., UK) containing 85% N 2 , 10% CO 2 , and 5% H 2 .

Techniques: Positive Control, Negative Control, Control

Journal: Frontiers in Microbiology

Article Title: Mucosal Prevalence and Interactions with the Epithelium Indicate Commensalism of Sutterella spp.

doi: 10.3389/fmicb.2016.01706

Figure Lengend Snippet: Comparison of Kdo 2 -lipid A biosynthesis enzymes between E. coli K12 and Sutterella spp.

Article Snippet: Sutterella wadsworthensis (DSM 14016 = ATCC 51579, type strain), S. parvirubra (DSM 19354 = YIT 11816 T , type strain) and S. stercoricanis (DSM 17807 = CCUG 47620 T , type strain) were obtained from German Collection of Microorganisms and Cell Cultures (DSMZ) and grown on Gifu anaerobic medium (GAM; Nissui Pharmaceutical Co., Ltd., Japan) agar for 2 days at 37°C in an anaerobic incubator (Concept Plus anaerobic workstation, Ruskinn Technology Ltd., UK) containing 85% N 2 , 10% CO 2 , and 5% H 2 .

Techniques: Comparison