Journal: Archivum Immunologiae et Therapiae Experimentalis

Article Title: Detection and Significance of Cytotoxic Cell Subsets in Biopsies of HCV-Infected Human Livers

doi: 10.1007/s00005-013-0258-6

Figure Lengend Snippet: Primary antibodies used

Article Snippet: NKG2D/CD314 , Mouse , Monoclonal , 1D11 , Novus Biologicals , NB100-65956.

Techniques:

Journal: BMC Cancer

Article Title: Suppression of Slit3 induces tumor proliferation and chemoresistance in hepatocellular carcinoma through activation of GSK3β/β-catenin pathway

doi: 10.1186/s12885-018-4326-5

Figure Lengend Snippet: Slit3 repression correlated with HCC tumor growth. a Tumor size of HCC patients cohort 1 ( n = 40) with high or low Slit3 expression. b Relative Slit3 expression inversely correlated with tumor size of HCC patients cohort 1 ( n = 40). c Tumor size of HCC patients cohort 2 ( n = 25) with high or low Slit3 expression. d Relative Slit3 expression inversely correlated with tumor size of HCC patients cohort 2 ( n = 25)

Article Snippet: Antibody against Slit3 was purchased from Novus Biologicals (Littleton, CO).

Techniques: Expressing

Journal: BMC Cancer

Article Title: Suppression of Slit3 induces tumor proliferation and chemoresistance in hepatocellular carcinoma through activation of GSK3β/β-catenin pathway

doi: 10.1186/s12885-018-4326-5

Figure Lengend Snippet: Slit3 negatively regulated HCC cell growth. a Expression of Slit3 in HCC cell-lines. b and c Stable Slit3 repressed clones (shSlit3) of LM3 and PLC cells displayed induced cell proliferation when compared with the control cells (shCTL) after 72 h. d Stable Slit3 overexpressed clones (Slit3) of Hep3B cells displayed impaired cell proliferation when compared with the control cells (pcDNA) after 72 h. The protein expression level was quantified by ImageJ software and normalized to the expression of actin. The experiment was repeated for three times and one representative blot was shown

Article Snippet: Antibody against Slit3 was purchased from Novus Biologicals (Littleton, CO).

Techniques: Expressing, Clone Assay, Control, Software

Journal: BMC Cancer

Article Title: Suppression of Slit3 induces tumor proliferation and chemoresistance in hepatocellular carcinoma through activation of GSK3β/β-catenin pathway

doi: 10.1186/s12885-018-4326-5

Figure Lengend Snippet: Molecular mechanism associated with stable Slit3 repression. Protein expression of certain proteins associated with cell cycle progression (cyclin D3), apoptosis (survivin) and GSK3β/β-catenin pathway (β-catenin and phosopho-GSK3β (ser9) in LM3 and PLC Slit3 shRNA transfectants (shSlit3) and shRNA control (shCTL). The protein expression level was quantified by ImageJ software and normalized to the expression of actin. The experiment was repeated for three times and one representative blot was shown

Article Snippet: Antibody against Slit3 was purchased from Novus Biologicals (Littleton, CO).

Techniques: Expressing, shRNA, Control, Software

Journal: BMC Cancer

Article Title: Suppression of Slit3 induces tumor proliferation and chemoresistance in hepatocellular carcinoma through activation of GSK3β/β-catenin pathway

doi: 10.1186/s12885-018-4326-5

Figure Lengend Snippet: Slit3 negatively regulated HCC cell growth in vivo. a Stable Slit3 repressed clones (LM3-shSlit3) displayed induced tumor growth when compared with the control cells (LM-shCTL) 6 weeks post-injection of cells subcutaneously into flank region of nude mice ( n = 5). b Stable Slit3 overexpressed clones (Hep3B-Slit3) displayed impaired tumor growth when compared with the control cells (Hep3B-pcDNA) 6 weeks post-injection of cells subcutaneously into flank region of nude mice ( n = 5). c Immunohistochemical staining of Slit3, CD31, p27, β-catenin and phosopho-GSK3β (ser9) in tumor formed by LM3-shCTL and LM3-shSlit3 stable cells. d Immunohistochemical staining of Slit3, CD31, p27, β-catenin and phosopho-GSK3β (ser9) in tumor formed by Hep3B-pcDNA and Hep3B-Slit3 stable cells. Representative results from two mice of each group were shown

Article Snippet: Antibody against Slit3 was purchased from Novus Biologicals (Littleton, CO).

Techniques: In Vivo, Clone Assay, Control, Injection, Immunohistochemical staining, Staining

Journal: BMC Cancer

Article Title: Suppression of Slit3 induces tumor proliferation and chemoresistance in hepatocellular carcinoma through activation of GSK3β/β-catenin pathway

doi: 10.1186/s12885-018-4326-5

Figure Lengend Snippet: Stable repression of Slit3 expression enhanced chemoresistance in HCC cells. (Left panel) LM3-shSlit3 and shCTL cells and (right panel) PLC-shSlit3 and shCTL cells were treated with ( a ) 10 μM sorafenib, ( b ) 10 μM oxaliplatin and ( c ) 100 μM 5-FU for 72 h. The number of cells was determined by MTT assay and expressed as percentage of that under vehicle control treatment. When compared with shCTL cells, shSlit3 cells showed significantly stronger resistance to the treatment of all chemotherapeutic drugs. Each experiment was performed in triplicate and data was obtained from three independent experiments

Article Snippet: Antibody against Slit3 was purchased from Novus Biologicals (Littleton, CO).

Techniques: Expressing, MTT Assay, Control

Journal: BMC Cancer

Article Title: Suppression of Slit3 induces tumor proliferation and chemoresistance in hepatocellular carcinoma through activation of GSK3β/β-catenin pathway

doi: 10.1186/s12885-018-4326-5

Figure Lengend Snippet: Molecular mechanism associated with Slit3 repression-induced chemoresistance. Protein expression of β-catenin, cyclin D3 and survivin in LM3 and PLC Slit3 shRNA transfectants (shSlit3) and shRNA control (shCTL) following 72-h vehicle control (C), 10 μM sorafenib (SOR), 10 μM oxaliplatin (OXA) and 100 μM 5-FU (5FU) treatment. The protein expression level was quantified by ImageJ software and normalized to the expression of actin. The experiment was repeated for three times and one representative blot was shown

Article Snippet: Antibody against Slit3 was purchased from Novus Biologicals (Littleton, CO).

Techniques: Expressing, shRNA, Control, Software

Journal: BMC Cancer

Article Title: Suppression of Slit3 induces tumor proliferation and chemoresistance in hepatocellular carcinoma through activation of GSK3β/β-catenin pathway

doi: 10.1186/s12885-018-4326-5

Figure Lengend Snippet: Down-regulation of β-catenin diminished the effects of Slit3-repression. Stable PLC Slit3-repressed cells (PLC-shSlit3) and control clone (PLC-shCTL) were transiently transfected with siRNA control (siCTL) or β-catenin siRNA (si-β-catenin) to investigate the effect of β-catenin down-regulation on Slit3-regulated effects. a PLC-shSlit3 siCTL cells showed significantly higher proliferation rate than PLC-shCTL siCTL cells whereas such induction was significantly impaired in PLC-shSlit3 si-β-catenin cells. b to d The induced chemoresistance in PLC-shSlit3 siCTL cells when compared with PLC-shCTL siCTL cells upon treatment of sorafenib, oxaliplatin and 5-FU were impaired in PLC-shSlit3 si-β-catenin cells. e The inductions in protein expression of phospho-GSK3β, cyclin D3 and survivin in PLC-shSlit3 siCTL cells when compared with PLC-shCTL siCTL were all reduced in PLC-shSlit3 si-β-catenin cells

Article Snippet: Antibody against Slit3 was purchased from Novus Biologicals (Littleton, CO).

Techniques: Control, Transfection, Expressing

Journal: BMC Cancer

Article Title: Suppression of Slit3 induces tumor proliferation and chemoresistance in hepatocellular carcinoma through activation of GSK3β/β-catenin pathway

doi: 10.1186/s12885-018-4326-5

Figure Lengend Snippet: Clinicopathological correlation of Slit3 expression in HCC patients

Article Snippet: Antibody against Slit3 was purchased from Novus Biologicals (Littleton, CO).

Techniques: Expressing, Over Expression, Infection

Journal: Advanced Science

Article Title: Cilia‐Mediated Insulin/Akt and ST2/JNK Signaling Pathways Regulate the Recovery of Muscle Injury

doi: 10.1002/advs.202202632

Figure Lengend Snippet: IL13 expression in FAPs is upregulated in Tchp −/− mice. A) qRT‐PCR analysis of RNA isolated from whole TA muscle of WT and Tchp −/− mice ( n = 3 or 4) at 3 dpi. B) Immunofluorescence for IL13 (red), hematopoietic cells (CD45, green), and nuclei (Hoechst33342, blue) in TA muscle cross‐sections of WT and Tchp −/− mice at 3 dpi. Lower magnification images are shown in Figure S6A, Supporting Information. Scale bars, 10 µm. C) Quantification of the IL4‐, IL13‐, or IL33‐stained area per 40× view in (B) and Figure S6A,B (Supporting Information) ( n = 6 or 7 fields each). D) Quantification of the IL13‐stained area per 40× view excluding the CD45‐stained area in (B) ( n = 7 fields each). E) Immunofluorescence for IL4 (red), IL13 (red), and IL33 (red), PDGFR α + (green), and nuclei (Hoechst33342, blue) in PDGFR α + FAPs isolated from hindlimbs of WT and Tchp −/‐ mice. Scale bars: 20 µm. All data are the mean ± S.D from 3 to 5 mice. * p < 0.05, ** p < 0.01, N.S., not significant; two‐tailed unpaired Student's t ‐tests.

Article Snippet: Staining was performed using the following antibodies: acetylated tubulin (6‐11B‐1, Sigma–Aldrich; 1:200), Arl13b (17711‐1‐AP, Proteintech; 1:200), PTRF (18892‐1‐AP, Proteintech; 1:100), Caveolin‐1 (#3238, Cell Signaling Technology; 1:300), Collagen I (ab21286, Abcam; 1:100), Perilipin (D1D8, Cell Signaling Technology; 1:200), CD45 and CD45‐biotin (30‐F11, Biolegend; 1:100), CD68 (FA‐11, Bio‐Rad; 1:100), CD206 (MR5D3, Bio‐Rad; 1:100), Desmin (D93F5, Cell Signaling Technology; 1:200), Flotillin‐2 (B‐6, Santa Cruz Biotechnology; 1:20), FOXP3 (FJK‐16s, Invitrogen; 1:100), GM3 (GMR6, Tokyo Chemical Industry; 1:500), IL4 (BVD4‐1D11, Novus Biologicals; 1:100), IL13 (AF‐413, R&D Systems; 1:100), IL33 (AF3626, R&D Systems; 1:100), PAX7 (MAB1675, R&D Systems; 1:100), PDGFR α (AF1062, R&D Systems; 1:200; APA5, Biolegend; 1:100), p‐Histone H3 (Ser 10) (sc‐8656‐R, Santa Cruz Biotechnology; 1:100), Sca1 (E13‐161.7, Biolegend, 1:100), Vimentin (#280618, R&D Systems; 1:200), Ki67 (SolA15, Invitrogen; 1:200), and Laminin (L9393, Sigma Aldrich; 1:200).

Techniques: Expressing, Quantitative RT-PCR, Isolation, Immunofluorescence, Staining, Two Tailed Test