Journal: Oncogene

Article Title: Exosomal circSPIRE1 mediates glycosylation of E-cadherin to suppress metastasis of renal cell carcinoma.

doi: 10.1038/s41388-023-02678-7

Figure Lengend Snippet: Fig. 6 CircSPIRE1 expression was dependent on the QKI positive feedback loop. A QKI was associated with the introns flanking circSPIRE1. RIP assays were conducted with a QKI antibody or control IgG, and primer sets covering the introns flanking circSPIRE1 (top). RNA enrichment indicates the levels of the indicated fragments in the anti-QKI precipitates relative to those in the IgG precipitates (bottom). Mean ± S.D. are provided. Dot plots reflect data points from three independent experiments. B Schematic structures showing RNA-binding sequence in the introns flanking the circSPIRE1 and a summary of intron truncations. C RNA pull-down assay performed to analyze the interaction between QKI and introns flanking circSPIRE1. D CircSPIRE1 expression upon QKI knockdown or overexpression. E Positive correlation between QKI expression and circSPIRE1 expression in 142 clinical samples of RCC. F Schematic diagram of mechanism of circSPIRE1 during RCC metastasis in RCC cells.

Article Snippet: The following primary antibodies were used: CD34 (1:200 dilution, ab81289, Abcam), CK (1:200 dilution, ab191208, Abcam), ELAVL1 (1:250 dilution, ab200342, Abcam), GALNT3 (1:100 dilution, AP51768PU-N, Origene), QKI (1:200 dilution, A300-183A, BETHYL), E-cadherin (1:250 dilution, ab40772, Abcam), and anti-Giantin (1:100 dilution, ab37266, Abcam).

Techniques: Expressing, Control, RNA Binding Assay, Sequencing, Pull Down Assay, Knockdown, Over Expression

Journal: EMBO reports

Article Title: ADAR1 promotes robust hypoxia signaling via distinct regulation of multiple HIF-1α-inhibiting factors.

doi: 10.15252/embr.201847107

Figure Lengend Snippet: Figure 5. ADAR1 regulates LIMD1 mRNA subcellular distribution and consequently its protein translation.

Article Snippet: Rabbit primary antibodies were used for detecting HIF-2a (Cell Signaling, 7096), PHD1 (GeneTex, GTX123033), PHD2 (GeneTex, GTX132293), LIMD1 (BETHYL, A303-182A), and VHL (BD Transduction Laboratories, 556347).

Techniques: