Journal: Ecotoxicology and environmental safety

Article Title: NAC antagonizes arsenic-induced neurotoxicity through TMEM179 by inhibiting oxidative stress in Oli-neu cells.

doi: 10.1016/j.ecoenv.2021.112554

Figure Lengend Snippet: Fig. 5. TMEM179 is expressed in OPCs. (a) Tmem179 gene expression in different cell types in adult mouse brain tissues. The data were obtained from the MCA database. (b) Immunofluorescence staining of NG2 and TMEM179 in the cerebral cortices of mice. Scale bar, 25 µm. (c) The subcellular localization of Tmem179 was assessed with a Tmem179 antibody and MitoTracker staining. Scale bar, 20 µm. (d, e) Measurement of Tmem179 expression after arsenic and NAC treatment for 24 h. Means ± SEMs, n = 3. *p < 0.05 and **p < 0.01, compared with the control group; #p < 0.05, compared with the arsenic-treated group, One-way ANOVA followed by Tukey’s multiple comparison test.

Article Snippet: The membranes were blocked for 1 h at room temperature with QuickBlockTM Blocking Buffer (Beyotime, China) and incubated with primary antibodies against TMEM179 (Proteintech, 24799-1-AP, 1:1000), cleaved CASPASE-3 (Cell Signaling Technology, 9664S, 1:1000), BCL-2 (Cell Signaling Technology, 3498T, 1:1000), BAX (Cell Signaling Technology, 2772 T, 1:1000), cleaved PARP (Cell Signaling Technology, 9544S, 1:1000), and ACTB (Sigma-Aldrich, A5441, 1:5000) at 4 ◦C overnight.

Techniques: Gene Expression, Immunofluorescence, Staining, Expressing, Control, Comparison

Journal: Ecotoxicology and environmental safety

Article Title: NAC antagonizes arsenic-induced neurotoxicity through TMEM179 by inhibiting oxidative stress in Oli-neu cells.

doi: 10.1016/j.ecoenv.2021.112554

Figure Lengend Snippet: Fig. 6. Tmem179 is a key factor mediating the protective effects of NAC. (a) Cell viability, (b) total ROS levels, and (c) mtROS levels measured after Tmem179 overexpression and silencing. (d, e) ATP levels and the mitochondrial membrane potential (ΔΨm) measured after Tmem179 overexpression and silencing. (f, g) Cell apoptosis evaluated after Tmem179 overexpression and silencing. Means ± SEMs, n = 3. ***p < 0.001, compared with the control group; #p < 0.05, ##p < 0.01, and ###p < 0.001, compared with the arsenic-treated group; $p < 0.05, $$p < 0.01, and $$$p < 0.001, compared with arsenic and NAC-treated group, One-way ANOVA followed by Tukey’s multiple comparison test.

Article Snippet: The membranes were blocked for 1 h at room temperature with QuickBlockTM Blocking Buffer (Beyotime, China) and incubated with primary antibodies against TMEM179 (Proteintech, 24799-1-AP, 1:1000), cleaved CASPASE-3 (Cell Signaling Technology, 9664S, 1:1000), BCL-2 (Cell Signaling Technology, 3498T, 1:1000), BAX (Cell Signaling Technology, 2772 T, 1:1000), cleaved PARP (Cell Signaling Technology, 9544S, 1:1000), and ACTB (Sigma-Aldrich, A5441, 1:5000) at 4 ◦C overnight.

Techniques: Over Expression, Membrane, Control, Comparison

Journal: Ecotoxicology and environmental safety

Article Title: NAC antagonizes arsenic-induced neurotoxicity through TMEM179 by inhibiting oxidative stress in Oli-neu cells.

doi: 10.1016/j.ecoenv.2021.112554

Figure Lengend Snippet: Fig. 7. PKC-β is a downstream factor of TMEM179. (a-g) Expression of apoptosis-related proteins (cleaved-PARP, BCL-2, BAX, cleaved-CASPASE-3) after TMEM179 overexpression and silencing. Means ± SEMs, n = 3. *p < 0.05, ** p < 0.01, and ***p < 0.001, compared with the control group; #p < 0.05, ##p < 0.01, and ###p < 0.001, compared with the arsenic-treated group; $p < 0.05, $$p < 0.01, and $$$p < 0.001, compared with the arsenic and NAC-treated group, One-way ANOVA followed by Tukey’s multiple comparison test.

Article Snippet: The membranes were blocked for 1 h at room temperature with QuickBlockTM Blocking Buffer (Beyotime, China) and incubated with primary antibodies against TMEM179 (Proteintech, 24799-1-AP, 1:1000), cleaved CASPASE-3 (Cell Signaling Technology, 9664S, 1:1000), BCL-2 (Cell Signaling Technology, 3498T, 1:1000), BAX (Cell Signaling Technology, 2772 T, 1:1000), cleaved PARP (Cell Signaling Technology, 9544S, 1:1000), and ACTB (Sigma-Aldrich, A5441, 1:5000) at 4 ◦C overnight.

Techniques: Expressing, Over Expression, Control, Comparison

Journal: Biosensors

Article Title: Pathway-like Activation of 3D Neuronal Constructs with an Optical Interface.

doi: 10.3390/bios15030179

Figure Lengend Snippet: Figure 1. PDMS device for culture of 3D cortical constructs with guided neurites in microchannels. (a) Schematics showing geometrical features of the PDMS device. (i) A longitudinal cross section containing one microchannel. The height of microchannel prevented neuron soma from entering but permitted neurite extension. (ii) Cross sections of two different devices used in this study. (iii) The size of the well for cell seeding. (b) Schematic showing the cell seeding protocol: (i) two PDMS layers (bottom one is the main layer and the top one serves as the mask layer) are stacked one day before cell seeding; (ii) high-density solution of dissociated neurons is placed into the well of the double-layer PDMS device; (iii) mask layer is peeled off after 24 h, removing excess cells; (iv) neural processes growing through channels during maturation. (c) Phase contrast and fluorescent images showing jRGECO1a expression in neural cells and Channelrhodopsin2 (ChR2) expression in axons and dendrites. (d) Axons enter and fill the channels: (i) phase contrast images (top) and fluorescent images of ChR2 expression (bottom) in devices with narrow (left) and wide (right) microchannels; (ii,iii) show 20X and 10X magnification images of wide channels with axons; (iv,v) show 20X and 10X magnification images of narrow channels with axons. All scale bars are 200 µm.

Article Snippet: On day-in vitro (DIV) 1, the culture medium was replaced with medium containing adeno-associated virus (AAV) particles, expressing a genetically encoded intercellular Biosensors 2025, 15, 179 5 of 14 calcium indicator jRGECO1a (a gift from Douglas and Kim GENIE, Addgene plasmid # 100854, Watertown, MA, USA) under the Synapsin promoter for optical recording.

Techniques: Construct, Expressing