Journal: Biochemical and biophysical research communications

Article Title: EP3 activation facilitates bladder excitability via HCN channels on ICCs.

doi: 10.1016/j.bbrc.2017.01.131

Figure Lengend Snippet: Fig. 2. Effect of EP3 on the expression and function of HCN channels. (A, D) Western blot analysis compared the expression of HCN channels between mouse bladders treated by intravesical instillation with sulprostone and mouse bladders treated with NS. GAPDH was used as the control (n ¼ 8). (B, C) Whole-cell patch clamping was used to determine the effects of PGE2 and sulprostone on the HCN channel current (n ¼ 6). (E) Single-cell PCR showing that the cells used for patch clamping were positive for the ICC marker c-Kit. *p < 0.05, **p < 0.01.

Article Snippet: PGE2 (10 6 M; Sigma, USA) and sulprostone (10 5 M; Santa Cruz, USA) were separately added to the bath.

Techniques: Expressing, Western Blot, Control, Marker

Journal: Biochemical and biophysical research communications

Article Title: EP3 activation facilitates bladder excitability via HCN channels on ICCs.

doi: 10.1016/j.bbrc.2017.01.131

Figure Lengend Snippet: Fig. 3. Effect of EP3 on the intracellular calcium of ICCs. (A, B) Nine images were chosen to represent the change in concentration of intracellular calcium in the ICCs after the agonist of EP3 and antagonist of HCN channels were added. (C, D, E, F) Real-time relative fluorescence intensities (RFI, F/F0) were depicted as continuous plots. PGE2, sulprostone and ZD7288 were applied to test their effects on the concentration of intracellular calcium in ICCs (n ¼ 8). *p < 0.05, **p < 0.01.

Article Snippet: PGE2 (10 6 M; Sigma, USA) and sulprostone (10 5 M; Santa Cruz, USA) were separately added to the bath.

Techniques: Concentration Assay

Journal: Biochemical and biophysical research communications

Article Title: EP3 activation facilitates bladder excitability via HCN channels on ICCs.

doi: 10.1016/j.bbrc.2017.01.131

Figure Lengend Snippet: Fig. 4. Effect of EP3 on the contraction of bladder detrusor strips. (A) Bladder detrusor strip test showing the effects of PGE2, sulprostone and ZD7288 on the amplitude of wild- type mouse bladder detrusors (n ¼ 8). (B) Effects of PGE2 and sulprostone on the amplitude of the HCN1/ mice bladder detrusor (n ¼ 8). (C) Effects of ZD7288, PGE2 and sulprostone on the amplitude of the rat bladder detrusor (n ¼ 8). (D) Bladder detrusor strip test revealing the effects of L798106 (an antagonist of EP3) and PGE2 on the amplitude of rat bladder detrusor (n ¼ 8). (E, F) Graphs showing the different effects of PGE2 and sulprostone on wild-type mouse bladders and HCN1/ mice bladders (n ¼ 8). *p < 0.05, **p < 0.01.

Article Snippet: PGE2 (10 6 M; Sigma, USA) and sulprostone (10 5 M; Santa Cruz, USA) were separately added to the bath.

Techniques: Stripping Membranes

Journal: Molecular Medicine Reports

Article Title: Prostaglandin E2 promotes human CD34 + cells homing through EP2 and EP4 in vitro

doi: 10.3892/mmr.2017.6649

Figure Lengend Snippet: Expression of CXCR4 following treatment with EP2A and EP4A in CD34 + cells. (A) CXCR4 mRNA expression was evaluated in the six groups of CD34 + cells. (B) Western blotting of CXCR4 protein expression in the six groups of CD34 + cells. Data are from three independent experiments *P<0.05, **P<0.01. CXCR4, C-X-C chemokine receptor type 4; EP2A, prostaglandin E2 receptor EP2 subtype agonist; CD, cluster of differentiation; PGE2, prostaglandin E2; EP2AA, prostaglandin E2 receptor EP2 subtype antagonist.

Article Snippet: The EP2A ONO-AE1-259, EP4A ONO-AE1-329 and EP4AA ONO-AE3-208 were provided by Japanese ONO Pharmaceutical Co., Ltd. (Osaka, Japan).

Techniques: Expressing, Western Blot

Journal: Molecular Medicine Reports

Article Title: Prostaglandin E2 promotes human CD34 + cells homing through EP2 and EP4 in vitro

doi: 10.3892/mmr.2017.6649

Figure Lengend Snippet: Expression of SDF-1a following treatment with EP2A and EP4A treatment in BMMSCs. (A) SDF-1a mRNA expression was evaluated in the six groups of human BMMSCs. (B) SDF-1a protein expression was evaluated in the six groups of human BMMSCs. Data are from three independent experiments. *P<0.05, **P<0.01, ***P<0.001. SDF-1a, stromal cell-derived factor 1α; EP2A, prostaglandin E2 receptor EP2 subtype agonist; BMMSC, bone marrow mesenchymal stem cell; PGE2, prostaglandin E2; EP2AA, prostaglandin E2 receptor EP2 subtype antagonist.

Article Snippet: The EP2A ONO-AE1-259, EP4A ONO-AE1-329 and EP4AA ONO-AE3-208 were provided by Japanese ONO Pharmaceutical Co., Ltd. (Osaka, Japan).

Techniques: Expressing, Derivative Assay

Journal: Molecular Medicine Reports

Article Title: Prostaglandin E2 promotes human CD34 + cells homing through EP2 and EP4 in vitro

doi: 10.3892/mmr.2017.6649

Figure Lengend Snippet: Migratory rate of the six groups of human cluster of differentiation 34 + cells towards (A) SDF-1a and (B) BMMSCs. Data are from three independent experiments. *P<0.05, **P<0.01. SDF-1a, stromal cell-derived factor 1α; EP2A, prostaglandin E2 receptor EP2 subtype agonist; BMMSC, bone marrow mesenchymal stem cell; PGE2, prostaglandin E2; EP2AA, prostaglandin E2 receptor EP2 subtype antagonist.

Article Snippet: The EP2A ONO-AE1-259, EP4A ONO-AE1-329 and EP4AA ONO-AE3-208 were provided by Japanese ONO Pharmaceutical Co., Ltd. (Osaka, Japan).

Techniques: Derivative Assay

Journal: BMC Pharmacology

Article Title: Cloning and expression of the rabbit prostaglandin EP2 receptor

doi: 10.1186/1471-2210-2-14

Figure Lengend Snippet: Competition binding profile of the EP 2 receptor expressed in COS-1 cells. Membranes were incubated with 1.5–2 nM [ 3 H]PGE 2 and varying concentrations of competitors. The data shown are from a single experiment performed in triplicate and are representative of three to four experiments. Panel A: ■ PGE 2 ; ▼ PGF 2a ; ▲ PGD 2 ; ◆ iloprost. Panel B: □ butaprost free acid; ?? AH13205; ▽ misoprostol; ◇ sulprostone.

Article Snippet: Sulprostone, misoprostol, iloprost and AH13205 were kindly provided by Dr. Harold Kluender of Miles Laboratories, P.J.

Techniques: Binding Assay, Incubation

Journal: BJA: British Journal of Anaesthesia

Article Title: Thromboelastometry-guided treatment algorithm in postpartum haemorrhage: a randomised, controlled pilot trial

doi: 10.1016/j.bja.2022.10.031

Figure Lengend Snippet: Use of procoagulant and uterotonic medications and other methods in treatment of postpartum haemorrhage. sd , standard deviation. ∗ P-value < 0.05.

Article Snippet: Sulprostone is a synthetic prostaglandin E2 derivative and a prostaglandin E3 agonist, a second-line therapy used to control uterine atony, which was diagnosed in 29.6% in the ROTEM group and 44.4% in the control group ( P =0.260).

Techniques: Medications, Standard Deviation, Significance Assay, Control

Journal: Arteriosclerosis, thrombosis, and vascular biology

Article Title: A Protective Role of Microsomal Prostaglandin E Synthase-1 Derived PGE 2 and the Endothelial EP4 Receptor in Vascular Responses to Injury

doi: 10.1161/ATVBAHA.118.310713

Figure Lengend Snippet: Mouse aortic endothelial cells (MAECs) were used for the study of cell proliferation in vitro. Cells from DKO (A) and wildtype (WT) mice (B) were stimulated with agonists for PGE2 receptors [AE1-329 (AE1), an EP4 agonist; Sulprostone (Sul), an EP1/3 agonist; Butaprost (Buta), an EP2 agonist], and relative proliferation is shown. WT ECs were treated with AE1 of different concentrations, with or without IL-1β (10 ng/mL), and proliferation was determined (C). GW627368X (GW, an EP4 antagonist) suppressed proliferation of IL-1β-stimulated MAECs (D). Effects of AE1 or GW on the proliferation of Ptges −/− ECs, stimulated with IL-1β (10 ng/mL) or not, were shown (E). Under IL-1β (10 ng/mL) stimulation, GW (an EP4 antagonist) but not L-798106 (an EP3 antagonist), PF-04418948 (an EP2 antagonist) or ONO-8130 (an EP1 antagonist), all at a concentration of 1 μmol/L, inhibited endothelial cell proliferation (F). The pro-proliferative effect of AE1 (G) and the anti-proliferative effect of GW (H) was prevented by SQ (SQ22536, an adenylate cyclase inhibitor; 200 μmol/L) or H 89 (H 89 2HCl, a PKA inhibitor; 10 μmol/L), but not by ESI-09 (an EPAC inhibitor; 10 μmol/L). Db-cAMP (I; a cell permeable cAMP analogue, 30 μmol/L) promoted endothelial proliferation, and this was also blunted by H89 2HCl (a PKA inhibitor). Endothelial proliferation was stimulated by iloprost (an IP agonist, 1 μmol/L) and inhibited by Cay10441 (an IP antagonist, 10 μmol/L) (J). The adhesion of leukocytes to ECs was inhibited by the EP4 agonist, AE1-329, and was promoted by GW, an EP4 antagonist (K). Db-cAMP treatment reduced leukocyte adhesion to ECs (L). All results are from at least three independent data sets. *P<0.05. **P<0.01; One-way ANOVA was used for data comparisons with Bonferroni’s (A, G-K), Dunnett’s (B) or Turkey’s (C-F) post-test. Student’s unpaired t-test was used in L.

Article Snippet: The reagents and their concentrations used in the proliferation studies were as follows: AE1-329 (0.1-1 μmol/L; Gifted by ONO Pharmaceutical Co., Ltd., Osaka, Japan), Butaprost (1 μmol/L; 13740; Cayman Chemical, MI, USA), Sulprostone (1 μmol/L; 14765; Cayman), GW627368X (0.1-1 μmol/L; HY-16963; MedChemExpress, NJ, USA), L-798106 (1 μmol/L; 11129; Cayman), iloprost (1 μmol/L; 18215; Cayman), Cay10441 (10 μmol/L; 10005186; Cayman), PF-04418948 (1 μmol/L; S7211; Selleck), ONO-8130 (1 μmol/L; 19118; Cayman), SQ22536 (200 μmol/L; S8283; Selleck), H89 2HCl (10 μmol/L; S1582; Selleck), ESI-09 (10 μmol/L; 19130; Cayman), db-cAMP (3-100 μmol/L; D0260; Sigma, Darmstadt, Germany) and misoprostol (10 μmol/L; 410004; National Institutes for Food and Drug Control, Beijing, China).

Techniques: In Vitro, Concentration Assay

Journal: Arteriosclerosis, thrombosis, and vascular biology

Article Title: A Protective Role of Microsomal Prostaglandin E Synthase-1 Derived PGE 2 and the Endothelial EP4 Receptor in Vascular Responses to Injury

doi: 10.1161/ATVBAHA.118.310713

Figure Lengend Snippet: Human microvascular endothelial cells were treated with 1 μmol/L of agonists (A) or antagonist (B) of the receptors of PGE2, and relative proliferation was determined. Agonists: AE1-329 (EP4), sulprostone (EP1/3) and butaprost (EP2). Antagonist: GW627368X (EP4), L-798106 (EP3), PF-04418948 (EP2) and ONO-8130 (EP1). Both results were from three independent data sets. *P<0.05, **P<0.01; One-way ANOVA with Dunnett’s post-test was used for data comparisons.

Article Snippet: The reagents and their concentrations used in the proliferation studies were as follows: AE1-329 (0.1-1 μmol/L; Gifted by ONO Pharmaceutical Co., Ltd., Osaka, Japan), Butaprost (1 μmol/L; 13740; Cayman Chemical, MI, USA), Sulprostone (1 μmol/L; 14765; Cayman), GW627368X (0.1-1 μmol/L; HY-16963; MedChemExpress, NJ, USA), L-798106 (1 μmol/L; 11129; Cayman), iloprost (1 μmol/L; 18215; Cayman), Cay10441 (10 μmol/L; 10005186; Cayman), PF-04418948 (1 μmol/L; S7211; Selleck), ONO-8130 (1 μmol/L; 19118; Cayman), SQ22536 (200 μmol/L; S8283; Selleck), H89 2HCl (10 μmol/L; S1582; Selleck), ESI-09 (10 μmol/L; 19130; Cayman), db-cAMP (3-100 μmol/L; D0260; Sigma, Darmstadt, Germany) and misoprostol (10 μmol/L; 410004; National Institutes for Food and Drug Control, Beijing, China).

Techniques:

Journal: The Cochrane Database of Systematic Reviews

Article Title: Uterotonic agents for preventing postpartum haemorrhage: a network meta‐analysis

doi: 10.1002/14651858.CD011689.pub3

Figure Lengend Snippet: Poeschmann 1991

Article Snippet: Funding source , Unclear risk , Sulprostone was supplied by Schering without charge but no other funding sources are reported..

Techniques: Sequencing, Selection

Journal: The Cochrane Database of Systematic Reviews

Article Title: Uterotonic agents for preventing postpartum haemorrhage: a network meta‐analysis

doi: 10.1002/14651858.CD011689.pub3

Figure Lengend Snippet: Van Selm 1995

Article Snippet: Funding source , Unclear risk , Sulprostone was supplied by Schering without charge but no other funding sources are reported..

Techniques: Coagulation, Sequencing, Selection, Staining

Journal: The Cochrane Database of Systematic Reviews

Article Title: Uterotonic agents for preventing postpartum haemorrhage: a network meta‐analysis

doi: 10.1002/14651858.CD011689.pub3

Figure Lengend Snippet: Norchi 1988

Article Snippet: Funding source , Unclear risk , Sulprostone was supplied by Schering without charge but no other funding sources are reported..

Techniques:

Journal: Molecules (Basel, Switzerland)

Article Title: Anti-Periodontitis Effects of Dendropanax morbiferus H.Lév Leaf Extract on Ligature-Induced Periodontitis in Rats.

doi: 10.3390/molecules28020849

Figure Lengend Snippet: Figure 3. Effects of DMWE on LPS-induced NO (□) and PGE2 (■) production in RAW 264.7 cells. LPS, liposaccharide; DMWE, hot-water extracts of DML. Different superscript letters (a, b, c, d) indicate statistical significance in nitric oxide (NO) production (p < 0.05). Different superscript letters (p, q, r, s) indicate statistical significance in prostaglandin E2 (PGE2) production (p < 0.05).

Article Snippet: Molecules 2023, 28, x FOR PEER REVIEW 12 of 16 linked immunosorbent assay (ELISA) PGE2 kit (R&D Systems Inc., Minneapolis, MN, USA).

Techniques: