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Cell Signaling Technology Inc synaptotagmin
RVG‐nDMC increases TH expression and DA synthesis in PD mice. A–C) Representative blots and quantification showing TH and DAT expression levels in the SN of Control, MPTP, and MPTP + RVG‐nDMC groups. n = 6 per group. D) Ultrastructural analysis of synaptic vesicles in the SN of the three groups. Scale bars, 500 nm. Black arrows show the morphological synaptic vesicles. E) Quantification of synaptic vesicles in the SN of the three groups. n = 10 per group. F) Typical HPLC chromatograms of DA, DOPAC, 5‐HT, and 5‐HIAA. G–J) The levels of DA, DOPAC, 5‐HT, and 5‐HIAA were assessed by HPLC‐MS/MS analysis. n = 4 per group. K–M) Representative blots and quantification showing Synapsin, Syntaxin, <t>Synaptotagmin,</t> and PSD‐95 expression levels in the SN of the three groups. n = 6 per group. Results are expressed as the mean ± SEM. ** p < 0.01 vs Control. ## p < 0.01, # p < 0.05 vs MPTP group. Statistical significance was determined by one‐way ANOVA and Tukey tests for post‐hoc comparisons.
Synaptotagmin, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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RVG‐nDMC increases TH expression and DA synthesis in PD mice. A–C) Representative blots and quantification showing TH and DAT expression levels in the SN of Control, MPTP, and MPTP + RVG‐nDMC groups. n = 6 per group. D) Ultrastructural analysis of synaptic vesicles in the SN of the three groups. Scale bars, 500 nm. Black arrows show the morphological synaptic vesicles. E) Quantification of synaptic vesicles in the SN of the three groups. n = 10 per group. F) Typical HPLC chromatograms of DA, DOPAC, 5‐HT, and 5‐HIAA. G–J) The levels of DA, DOPAC, 5‐HT, and 5‐HIAA were assessed by HPLC‐MS/MS analysis. n = 4 per group. K–M) Representative blots and quantification showing Synapsin, Syntaxin, Synaptotagmin, and PSD‐95 expression levels in the SN of the three groups. n = 6 per group. Results are expressed as the mean ± SEM. ** p < 0.01 vs Control. ## p < 0.01, # p < 0.05 vs MPTP group. Statistical significance was determined by one‐way ANOVA and Tukey tests for post‐hoc comparisons.

Journal: Advanced Science

Article Title: Trojan Horse Delivery of 4,4′‐Dimethoxychalcone for Parkinsonian Neuroprotection

doi: 10.1002/advs.202004555

Figure Lengend Snippet: RVG‐nDMC increases TH expression and DA synthesis in PD mice. A–C) Representative blots and quantification showing TH and DAT expression levels in the SN of Control, MPTP, and MPTP + RVG‐nDMC groups. n = 6 per group. D) Ultrastructural analysis of synaptic vesicles in the SN of the three groups. Scale bars, 500 nm. Black arrows show the morphological synaptic vesicles. E) Quantification of synaptic vesicles in the SN of the three groups. n = 10 per group. F) Typical HPLC chromatograms of DA, DOPAC, 5‐HT, and 5‐HIAA. G–J) The levels of DA, DOPAC, 5‐HT, and 5‐HIAA were assessed by HPLC‐MS/MS analysis. n = 4 per group. K–M) Representative blots and quantification showing Synapsin, Syntaxin, Synaptotagmin, and PSD‐95 expression levels in the SN of the three groups. n = 6 per group. Results are expressed as the mean ± SEM. ** p < 0.01 vs Control. ## p < 0.01, # p < 0.05 vs MPTP group. Statistical significance was determined by one‐way ANOVA and Tukey tests for post‐hoc comparisons.

Article Snippet: Anti‐synapsin І (#5297), Iba1(#17198), synaptotagmin (#14558), syntaxin (#18572), PSD‐95 (#3450), and Bax (#14796) antibodies were purchased from Cell Signaling Technology (Danvers, MA, USA).

Techniques: Expressing, Control, Tandem Mass Spectroscopy